- 著者
- Junna Oba Hiroaki Taniguchi Masae Sato Reika Takamatsu Satoru Morikawa Taneaki Nakagawa Hiromasa Takaishi Hideyuki Saya Koichi Matsuo Hiroshi Nishihara
- 出版者
- The Keio Journal of Medicine
- 雑誌
- The Keio Journal of Medicine (ISSN:00229717)
- 巻号頁・発行日
- vol.70, no.2, pp.35-43, 2021 (Released:2021-06-25)
- 参考文献数
- 52
- 被引用文献数
- 2 21
The year 2020 will be remembered for the coronavirus disease 2019 (COVID-19) pandemic, which continues to affect the whole world. Early and accurate identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is fundamental to combat the disease. Among the current diagnostic tests, real-time reverse transcriptase–polymerase chain reaction (RT-qPCR) is the most reliable and frequently used method. Herein, we discuss the interpretation of RT-qPCR results relative to viral infectivity. Although nasopharyngeal swab samples are often used for RT-qPCR testing, they require collection by trained medical staff. Saliva samples are emerging as an inexpensive and efficient alternative for large-scale screening. Pooled-sample testing of saliva has been applied for mass screening of SARS-CoV-2 infection. Current policies recommend isolating people with borderline cycle threshold (Ct) values (35<Ct <40), despite these Ct values indicating minimal infection risk. We propose the new concept of a “social cut-off” Ct value and risk stratification based on the correlation of Ct with infectivity. We also describe the experience of RT-qPCR screening of saliva samples at our institution. It is important to implement a scientific approach to minimize viral transmission while allowing economic and social activities to continue.
- 著者
- Junna Oba Hiroaki Taniguchi Masae Sato Reika Takamatsu Satoru Morikawa Taneaki Nakagawa Hiromasa Takaishi Hideyuki Saya Koichi Matsuo Hiroshi Nishihara
- 出版者
- The Keio Journal of Medicine
- 雑誌
- The Keio Journal of Medicine (ISSN:00229717)
- 巻号頁・発行日
- pp.2021-0003-OA, (Released:2021-03-19)
- 参考文献数
- 52
- 被引用文献数
- 2 21
The year 2020 will be remembered for the coronavirus disease 2019 (COVID-19) pandemic, which continues to affect the whole world. Early and accurate identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is fundamental to combat the disease. Among the current diagnostic tests, real-time reverse transcriptase–polymerase chain reaction (RT-qPCR) is the most reliable and frequently used method. Herein, we discuss the interpretation of RT-qPCR results relative to viral infectivity. Although nasopharyngeal swab samples are often used for RT-qPCR testing, they require collection by trained medical staff. Saliva samples are emerging as an inexpensive and efficient alternative for large-scale screening. Pooled-sample testing of saliva has been applied for mass screening of SARS-CoV-2 infection. Current policies recommend isolating people with borderline cycle threshold (Ct) values (35<Ct <40), despite these Ct values indicating minimal infection risk. We propose the new concept of a “social cut-off” Ct value and risk stratification based on the correlation of Ct with infectivity. We also describe the experience of RT-qPCR screening of saliva samples at our institution. It is important to implement a scientific approach to minimize viral transmission while allowing economic and social activities to continue.
- 著者
- Masa-aki Kishida Satoshi Muratsugu Ryota Sakamoto Tetsuro Kusamoto Hiroshi Nishihara
- 出版者
- (社)日本化学会
- 雑誌
- Chemistry Letters (ISSN:03667022)
- 巻号頁・発行日
- pp.121264, (Released:2013-03-19)
- 被引用文献数
- 7
We prepared dimethyldihydropyrene (DHP) derivative 2 which possesses two ferrocenyl groups at the 4 and 9 positions through ethynylene linkers. 2 showed intense electronic communication between the two ferrocenyl groups, which is promoted by the highly developed π-orbital of the diethynylDHP moiety.
- 著者
- Shoko Suzuki Ryota Sakamoto Hiroshi Nishihara
- 出版者
- (社)日本化学会
- 雑誌
- Chemistry Letters (ISSN:03667022)
- 巻号頁・発行日
- vol.42, no.1, pp.17-18, 2013-01-05 (Released:2012-12-22)
- 参考文献数
- 23
- 被引用文献数
- 3
[Ru(tpy)(picSO)]BF4 (tpy: 2,2′:6′,2′′-terpyridine, picSO: 6-[(methylsulfinyl)methyl]picolinate) undergoes profound linkage isomerization behavior between stable S-bound and metastable O-bound isomers. S-[RuII(tpy)(picSO)]BF4 experiences photoisomerization to O-[RuII(tpy)(picSO)]BF4 upon irradiation with 436 nm light, whereas the back reaction is triggered with 546 nm light. Electrochemical linkage isomerization also takes place.
- 著者
- Satoru Tsukada Ryota Sakamoto Hiroshi Nishihara
- 出版者
- The Chemical Society of Japan
- 雑誌
- Chemistry Letters (ISSN:03667022)
- 巻号頁・発行日
- vol.41, no.4, pp.357-359, 2012-04-05 (Released:2012-03-17)
- 参考文献数
- 43
- 被引用文献数
- 4
[{Cp*Rh(S2C6H4)}2W(CO)2] (Cp*: η5-C5Me5) (1) and [{Cp*Rh(S2C6H4)}(S2C6H4)W(CO)2] (2) were synthesized by the reaction of rhodadithiolene [Cp*Rh(S2C6H4)] with [W(CO)3(py)3], and their crystal structures and physical properties were investigated. The formation mechanism of 2 from 1 was pursued by 1H NMR spectroscopy. 1 reacted with an excess amount of BF3·Et2O to furnish 2, indicating that a Lewis acid assisted the demetalation reaction.
- 著者
- Ryota Sakamoto Koya Prabhakara Rao Hiroshi Nishihara
- 出版者
- The Chemical Society of Japan
- 雑誌
- Chemistry Letters (ISSN:03667022)
- 巻号頁・発行日
- vol.40, no.12, pp.1316-1326, 2011-12-05 (Released:2011-11-26)
- 参考文献数
- 94
- 被引用文献数
- 22
In this account we describe ethynylene-conjugated arylethynylanthraquinone (ArAq) and bis(arylethynyl)anthraquinone (Ar2Aq) which possess ferrocene, triarylamine, platinadithiolene complex, p-methoxybenzene, p-methylbenzene, m-methylbenzene, and benzene as electron-donating arenes (Ar), and anthraquinone (Aq) as an electron acceptor. This series of compounds features donor–acceptor (D–A) interactions: (1) Expression of intramolecular charge-transfer (ICT) transitions; (2) flexible single crystal networks constructed by intermolecular D–A π–π stacking, which undergo guest-induced reversible crystal-to-crystal transformations. In addition, ArAq and Ar2Aq experience proton-induced cyclization so as to produce pyrylium and dipyrylium salts. These salts feature further expanded π-conjugation and stronger D–A interaction: (1) More red-shifted ICT transitions and narrower HOMO–LUMO gaps; (2) bright fluorescence; (3) valence tautomerism (VT); (4) unique reactivities.