著者

Toyotaka SATO Shin-ichi YOKOTA Torahiko OKUBO Kanako ISHIHARA Hiroshi UENO Yasukazu MURAMATSU Nobuhiro FUJII Yutaka TAMURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0186, (Released:2012-11-14)

被引用文献数

8 30

---

Fluoroquinolone resistance is mainly caused by mutations in quinolone resistance-determining regions of DNA gyrase and topoisomerase IV in Escherichia coli. The AcrAB-TolC efflux pump contributes to resistance against fluoroquinolone and other antimicrobials. In this study, we investigated a high-level mechanism of fluoroquinolone resistance in E. coli that was isolated from human clinical samples and canine fecal samples. E. coli strains with high levels of fluoroquinolone resistance have been found to be frequently resistant to cephalosporins. Strains with high-level fluoroquinolone resistance exhibited lower intracellular enrofloxacin (ENR) concentrations, higher expression of AcrA, and a greater reduction in the fluoroquinolone minimum inhibitory concentration for treatment with an efflux pump inhibitor. The frequency of strains with enhanced ENR resistance selection and the survival rate of E. coli in the presence of ENR in vitro were correlated well with AcrA protein expression levels in the parental strains. These results suggest that AcrAB-TolC efflux pump over-expression is related to high-level fluoroquinolone resistance and the selection of strains with enhanced fluoroquinolone resistance.

著者

Hiroshi UENO Osamu YAMATO Takeshi SUGIURA Moeko KOHYAMA Akira YABUKI Kenjiro MIYOSHI Kazuya MATSUDA Tsuyoshi UCHIDE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.15-0281, (Released:2015-08-03)

被引用文献数

1 3

---

A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors, visual disorder and seizure after 4 months of age. Finally, the cat died of neurological deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis. Histologically, swollen neurons characterized by accumulation of pale, slightly granular cytoplasmic materials were observed throughout the central nervous system. Dysmyelination or demyelination and gemistocytic astrocytosis were observed in the white matter. Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons. However, genetic analysis did not identify the c.1448G>C mutation, which is the single known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a new variant of the feline disease.

著者

Kei KONDOH Ayman ATIBA Kiyoshi NAGASE Shizuko OGAWA Takashi MIWA Teruya KATSUMATA Hiroshi UENO Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0279, (Released:2015-04-04)

被引用文献数

1

---

In the present study, we compare a new carbon dioxide (CO2) absorbent, Yabashi lime® with a conventional CO2 absorbent, Sodasorb® as a control CO2 absorbent for Compound A (CA) and Carbon monoxide (CO) productions. Four dogs were anesthetized with sevoflurane. Each dog was anesthetized with four preparations, Yabashi lime® with high or low-flow rate of oxygen and control CO2 absorbent with high or low-flow rate. CA and CO concentrations in the anesthetic circuit, canister temperature, and carbooxyhemoglobin (COHb) concentration in the blood were measured. Yabashi lime® did not produce CA. Control CO2 absorbent generated CA and its concentration was significantly higher in low-flow rate than a high-flow rate. CO was generated only in low-flow rate groups, but there was no significance between Yabashi lime® groups and control CO2 absorbent groups. However, the CO concentration in the circuit could not be detected (≤5 ppm) and no change was found in COHb level. Canister temperature was significantly higher in low-flow rate groups than high-flow rate groups. Furthermore, in low-flow rate groups, the lower layer of canister temperature in control CO2 absorbent group was significantly higher than Yabashi lime® group. CA and CO productions are thought to be related to the composition of CO2 absorbent, flow rate, and canister temperature. Though CO concentration is equal, it might be safer to use Yabashi lime® with sevoflurane anesthesia in dogs than conventional CO2 absorbent at the point of CA production.

著者

Kimiko Sasaki Kaori Hamano Hiroshi Ueno

出版者

Japan Science Society of Biological Macromolecules

雑誌

Journal of Biological Macromolecules (ISSN:13472194)

巻号頁・発行日

vol.14, no.1, pp.43-57, 2014 (Released:2018-09-30)

参考文献数

19

被引用文献数

1 2

---

Food extracts from spices and teas were examined for the taste altering ability on sweet, umami and bitter tastes by using the taste sensation test. The idea arose based upon the findings that 1) some food extracts from spices and teas act as salt taste enhancers and 2) the enhancing effect is directly proportional to glutamate decarboxylase 67 (GAD67) activity in vitro. Our results indicated that those extracts significantly activated or inhibited GAD67 enzyme activity were able to alter sweet, umami, and bitter tastes. However, there were no relationships between the taste potency of each of the examined extracts and the GAD67 relative activity ratio. While it is unlikely that the extracts have directly activated GAD67 activity and enhanced sweet, umami or bitter tastes, the results do not exclude the idea that GABA may participate in the taste cell-to-cell communications.

著者

Hiroshi UENO Tomohito ITOH Toyohiko NASUNO Wataru KONNO Atsushi KONDO Ikuo KONISHI Hisao INUKAI Daiki KOKUBO Mitsuhiro ISAKA Md Shafiqul ISLAM Osamu YAMATO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.23-0091, (Released:2023-07-27)

---

The mutant allele frequency of the Pyruvate kinase (PK) gene has been investigated mostly in pure breed cats. We investigated the PK mutant gene in stray and animal hoarding mongrel cats in Hokkaido, Japan. We also investigated the kinship of individuals carrying the mutant gene. Genotyping was conducted using the previously reported real-time PCR method. Fourteen microsatellite markers were used to identify the parents and offspring of cats carrying the PK mutant gene, and some kinship such as parent-offspring and siblings was observed. Some stray and animal hoarding cats carried the PK mutation gene and that consanguinity was confirmed among these cats indicated that the PK mutation gene was spread by unregulated interbreeding.

著者

Hiroshi Ueno

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

vol.86, no.11, pp.1745-1747, 2022-10-25 (Released:2022-10-25)

参考文献数

12

著者

Raymond N. Burton-Smith Jun Tsunoda Chihong Song Hiroshi Ueno Takeshi Murata Ryota Iino Kazuyoshi Murata

雑誌

第60回日本生物物理学会年会

巻号頁・発行日

2022-09-02

著者

Hinako ITO Hiroe KIKUZAKI Hiroshi UENO

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.65, no.3, pp.264-271, 2019-06-30 (Released:2019-06-30)

参考文献数

11

被引用文献数

18

---

Vegetables are rich sources of nutrients such as fiber, minerals, vitamins, and antioxidants. Vegetables also contain various free-form amino acids, which improves their nutritional and palatable value. Cooking alters the content of free amino acids in vegetables, which affects their nutritional values. In this study, free amino acid levels were evaluated after cooking vegetables by different methods, boiling, roasting in an oven, and using a microwave. Results showed that many vegetables analyzed contain aspartate and glutamine abundantly. On the other hand, hydroxyproline, cysteine, ornithine and citrulline are the free amino acids existing at low or undetectable levels in all vegetables tested. The total free amino acid content in vegetables tended to decrease after boiling, and almost the same amount of free amino acids was obtained in the cooking liquid. Roasting of vegetables in an oven resulted in an increase in the content of specific amino acids, including γ-aminobutyric acid (GABA). Thus, it is important to choose the right cooking methods to prevent the loss of free amino acids. The results of the present study emphasize the changes in the contents of free amino acids during cooking with methods that are typically used on a daily basis. Our study on the dynamics of free amino acids caused by various cooking methods provides ample information for future nutritional studies.

著者

Ayman ATIBA Hiroshi UENO Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.5, pp.583-589, 2011 (Released:2011-05-31)

参考文献数

32

被引用文献数

11 55

---

Delayed wound healing is one of the complications of diabetes mellitus. The present study was performed to investigate the effect of Aloe vera oral administration on open wounds in type 2 diabetic rats. Full thickness open wounds (1.5 × 1.5 cm) were created under general anesthesia on the backs of the rats. These rats were divided into two group, a control group (Group C) and an Aloe vera oral administration group (Group A). Each wound area was measured on days 1, 2, 4 and 8 postwounding. The stages of wound granulation tissues were evaluated histopathologically. The expression of transforming growth factor (TGF)-β1 and vascular endothelial growth factor (VEGF) were determined by immunohistochemically. The wounds were significantly contracted in Group A on days 2, 4 and 8 postwounding. Histological results revealed that the inflammatory cell infiltration, angiogenesis, extracellular matrix deposition and epithelialization were promoted in Group A, respectively. The immunohistochemical results revealed that both TGF-β1 and VEGF protein-positive cells increased in Group A on day 4 postwounding. We concluded that Aloe vera oral administration accelerated wound healing in type 2 diabetic rats.

著者

Hiroshi UENO Kenjiro MIYOSHI Sho FUKUI Yumi KONDO Kazuya MATSUDA Tsuyoshi UCHIDE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0159, (Released:2014-01-13)

被引用文献数

2 14

---

An 8-year-old neutered female Cavalier King Charles Spaniel was evaluated for progressing right forelimb lameness. Upon magnetic resonance imaging, the right-sided radius nerves and the caudal brachial plexus were swelled. The histological and molecular biological diagnosis of partial the C8 spinal nerve was T-cell lymphoma. Co-administration of lomustine and irradiation was started. However, this therapy was ineffective. At necropsy, neoplastic tissues were seen extending into the subarachnoid space of the spinal cord, the liver, pancreas and kidneys as gross findings. A large mass was also identified occupying the caudal thorax. Histologic findings included infiltration in these organs and mass by neoplastic lymphocytes. To date, involvement of peripheral nerves (neurolymphomatosis) is rarely reported in veterinary species.

著者

Toyotaka SATO Shin-ichi YOKOTA Torahiko OKUBO Kanako ISHIHARA Hiroshi UENO Yasukazu MURAMATSU Nobuhiro FUJII Yutaka TAMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.75, no.4, pp.407-414, 2013 (Released:2013-05-02)

参考文献数

38

被引用文献数

17 30

---

Fluoroquinolone resistance is mainly caused by mutations in quinolone resistance-determining regions of DNA gyrase and topoisomerase IV in Escherichia coli. The AcrAB-TolC efflux pump contributes to resistance against fluoroquinolone and other antimicrobials. In this study, we investigated a high-level mechanism of fluoroquinolone resistance in E. coli that was isolated from human clinical samples and canine fecal samples. E. coli strains with high levels of fluoroquinolone resistance have been found to be frequently resistant to cephalosporins. Strains with high-level fluoroquinolone resistance exhibited lower intracellular enrofloxacin (ENR) concentrations, higher expression of AcrA, and a greater reduction in the fluoroquinolone minimum inhibitory concentration for treatment with an efflux pump inhibitor. The frequency of strains with enhanced ENR resistance selection and the survival rate of E. coli in the presence of ENR in vitro were correlated well with AcrA protein expression levels in the parental strains. These results suggest that AcrAB-TolC efflux pump over-expression is related to high-level fluoroquinolone resistance and the selection of strains with enhanced fluoroquinolone resistance.