著者

Yunosuke YUCHI Ryohei SUZUKI Takahiro TESHIMA Hirotaka MATSUMOTO Hidekazu KOYAMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.21-0195, (Released:2021-11-04)

被引用文献数

8

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Pulmonary hypertension (PH) is a common comorbidity in dogs with myxomatous mitral valve disease (MMVD), and can induce various changes in the right heart, such as right ventricular (RV) hypertrophy, dilatation, and dysfunction. We hypothesized that RV function, not only systolic function but also diastolic function, could be worsened with PH progression. We aimed to compare RV systolic and diastolic function in dogs with MMVD. Twenty healthy dogs and sixty-eight dogs with MMVD were enrolled. Dogs with MMVD were classified into the probability of PH. Two-dimensional and Doppler echocardiographic indices for right heart and two-dimensional speckle tracking echocardiography indices were measured. The morphological indicators of the right heart were significantly higher only in the high probability of PH group. The RV strain, early-diastolic and systolic strain rates were significantly lower in the high probability of PH group than those in the low and intermediate probability of PH groups. Multivariate analysis showed that increased RV internal dimension normalized by body weight and RV myocardial performance index were significantly associated with the presence of right-sided congestive heart failure. Speckle tracking echocardiography-derived RV systolic and diastolic function were activated in the low and intermediate probability of PH groups. However, dogs with high probability of PH showed RV myocardial dysfunction and dilatation. Increased RV myocardial performance index and end-diastolic RV internal dimension normalized by body weight were significantly associated with the presence of right-sided congestive heart failure in dogs with MMVD.

著者

Yohei MOCHIZUKI Ryohei SUZUKI Yuyo YASUMURA Takahiro SAITO Takahiro TESHIMA Hirotaka MATSUMOTO Hidekazu KOYAMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0718, (Released:2019-04-02)

被引用文献数

3

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Beta-blockers are used to treat cats with hypertrophic obstructive cardiomyopathy (HOCM). However, there are various hemodynamic responses to beta-blockers. This retrospective study aimed to explore the relationship between the response to carvedilol and the presence of geometric abnormalities. Medical records were reviewed for 16 cats diagnosed with HOCM. Cats were divided into two groups based on the velocity of the left-ventricular outflow-tract after carvedilol treatment (responder: eight cats, non-responder: eight cats). Baseline intergroup comparison revealed that anterior mitral valve leaflet length and diastolic left-ventricular posterior-wall thickness were significantly greater in the non-responder group. Longer anterior mitral valve leaflet and thicker left-ventricular posterior-wall may cause poor response to carvedilol. Thus, these properties may predict a lack of response to carvedilol therapy.

著者

Ayaka NIINA Ryoko KIBE Ryohei SUZUKI Yunosuke YUCHI Takahiro TESHIMA Hirotaka MATSUMOTO Yasushi KATAOKA Hidekazu KOYAMA

出版者

BMFH Press

雑誌

Bioscience of Microbiota, Food and Health (ISSN:21863342)

巻号頁・発行日

vol.40, no.2, pp.98-104, 2021 (Released:2021-04-17)

参考文献数

47

被引用文献数

20

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In human medicine, fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridioides difficile infection. It has also been tested as a treatment for multiple gastrointestinal diseases, including inflammatory bowel disease (IBD). However, only a few studies have focused on the changes in the microbiome following FMT for canine IBD. Here, we performed FMT in nine dogs with IBD using the fecal matter of healthy dogs and investigated the subsequent changes in the fecal microbiome and clinical signs. In three dogs, the fecal microbiome was examined by 16S rRNA sequencing. Fusobacteria were observed at a low proportion in dogs with IBD. However, the post-FMT microbiome became diverse and showed a significant increase in Fusobacteria proportion. Fusobacterium was detected in the nine dogs by quantitative polymerase chain reaction. The proportion of Fusobacterium in the post-FMT fecal microbiome was significantly increased (p<0.05). The changes in clinical signs (e.g., vomiting, diarrhea, and weight loss) were evaluated according to the canine inflammatory bowel disease activity index. The score of this index significantly decreased in all dogs (p<0.05) with improvements in clinical signs. These improvements were related to the changes in the proportion of microbes, particularly the increase in Fusobacterium. The dogs with IBD showed a lower proportion of Fusobacterium than healthy dogs. This suggests that a low proportion of Fusobacterium is a characteristic feature of canine IBD and that Fusobacterium is involved in this disease. The results of this study may help elucidate the pathogenesis of this disease and its association with Fusobacterium.

著者

Eli Kaminuma Yukino Baba Masahiro Mochizuki Hirotaka Matsumoto Haruka Ozaki Toshitsugu Okayama Takuya Kato Shinya Oki Takatomo Fujisawa Yasukazu Nakamura Masanori Arita Osamu Ogasawara Hisashi Kashima Toshihisa Takagi

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

pp.19-00034, (Released:2020-03-26)

参考文献数

37

被引用文献数

3

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Recently, the prospect of applying machine learning tools for automating the process of annotation analysis of large-scale sequences from next-generation sequencers has raised the interest of researchers. However, finding research collaborators with knowledge of machine learning techniques is difficult for many experimental life scientists. One solution to this problem is to utilise the power of crowdsourcing. In this report, we describe how we investigated the potential of crowdsourced modelling for a life science task by conducting a machine learning competition, the DNA Data Bank of Japan (DDBJ) Data Analysis Challenge. In the challenge, participants predicted chromatin feature annotations from DNA sequences with competing models. The challenge engaged 38 participants, with a cumulative total of 360 model submissions. The performance of the top model resulted in an area under the curve (AUC) score of 0.95. Over the course of the competition, the overall performance of the submitted models improved by an AUC score of 0.30 from the first submitted model. Furthermore, the 1st- and 2nd-ranking models utilised external data such as genomic location and gene annotation information with specific domain knowledge. The effect of incorporating this domain knowledge led to improvements of approximately 5%–9%, as measured by the AUC scores. This report suggests that machine learning competitions will lead to the development of highly accurate machine learning models for use by experimental scientists unfamiliar with the complexities of data science.

著者

Eli Kaminuma Yukino Baba Masahiro Mochizuki Hirotaka Matsumoto Haruka Ozaki Toshitsugu Okayama Takuya Kato Shinya Oki Satoshi Ogasawara Hisashi Kashima Toshihisa Takagi

出版者

The Molecular Biology Society of Japan

雑誌

The 39th Annual Meeting of the Molecular Biology Society of Japan

巻号頁・発行日

2016-11-08

著者

Hiroki YOSHIMATSU Hirotaka MATSUMOTO Masanari MINAMOTO Ryohei SUZUKI Yohei MOCHIZUKI Takahiro TESHIMA Hidekazu KOYAMA

出版者

日本獣医循環器学会

雑誌

動物の循環器 (ISSN:09106537)

巻号頁・発行日

vol.49, no.1, pp.11-16, 2016-06-15 (Released:2016-07-07)

参考文献数

12

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Fatty acids are a major source of energy in the normal myocardium and are taken up passively from the bloodstream. However, with heart failure, the use of fatty acids decreases in the myocardium. Therefore, serum fatty acid concentrations in dogs with mitral insufficiency (MI) might differ from those in normal dogs. The present study was designed to determine the serum fatty acid compositions in dogs with different severities of MI according to the classification proposed by the International Small Animal Cardiac Heart Council (ISACHC) and to elucidate the relationships between the determined compositions and echocardiographic parameters. In total, 30 dogs with MI were divided into 3 groups (I, II, and III) according to the ISACHC classification of MI severity. The healthy group consisted of 12 dogs matched with the MI groups for age and body weight. The serum concentrations of 13 fatty acids were measured by gas chromatography. The levels of linoleic acid (LA), docosatetraenoic acid (DTA), and arachidonic acid (AA) in group I were significantly lower than those in the healthy group (p<0.05). The levels of eicosapentaenoic acid (EPA) and AA in group II were significantly lower than those in the healthy group (p<0.05). The level of EPA in group III was significantly lower than that in the healthy group (p<0.05). In addition, the ratio of EPA to AA levels (EPA/AA ratio) in group III was significantly lower than that in the healthy group (p<0.05). With regard to the relationships between fatty acid concentrations in all MI groups (I through III) and echocardiographic parameters, the levels of AA and DTA showed a significant positive correlation with the ratio of left ventricular end-diastolic internal diameter to aortic diameter (LVIDd/Ao ratio) (AA, r=0.396 and p=0.048; DTA, r=0.426 and p=0.027). In addition, the docosapentaenoic acid (DPA) level correlated negatively with fractional shortening (r=-0.437 and p=0.023). Furthermore, the EPA/AA ratio correlated negatively with the ratio of left atrial to aortic diameters (r=-0.383 and p=0.048). The fatty acid concentrations and ratios in the dogs with 3 types of MI according to the ISACHC classification differed from those in healthy dogs. Some of these concentrations and ratios correlated with echocardiographic parameters.

著者

Ryuji FUKUSHIMA Rieko YOSHIYUKI Noboru MACHIDA Hirotaka MATSUMOTO Soomin KIM Lina HAMABE Hsu HUAI-CHE Toshiharu FUKAYAMA Shuji SUZUKI Derya AYTEMIZ Ryo TANAKA Yoshihisa YAMANE

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0319, (Released:2013-04-05)

被引用文献数

1 5

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A four-month-old female Labrador retriever was brought to the Tokyo University of Agriculture and Technology Animal Medical Center for examination of its main symptoms of cough, tachypnea and exercise intolerance. Upon examination, the dog was found to have cyanosis and inadequate growth. Echocardiography revealed tetralogy of Fallot. Cardiac catheterization confirmed that the main pulmonary artery was completely occluded and that blood flowed from the aorta to the pulmonary artery. Accordingly, the animal was diagnosed with extreme tetralogy of Fallot.