著者

Haruka Sato Natsumi Kawato Furong Li Kanto Ito Ami Shinoda Taichi Hasunuma Jiayi Liu Yasuyuki Kawaharada Chikako Nishitani Masato Wada Manabu Watanabe Sadao Komori

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.QH-041, (Released:2023-05-31)

---

Shoot regeneration experiments from growth-point-derived callus were conducted to improve apple genome editing techniques. The plant material studied was in vitro maintained shoots of ‘Fuji’. In the first step, a procedure for shoot formation from apple callus was established. After using the two earlier reported cases’ experimental procedures and media compositions, we investigated the effect of media variations in callus induction, callus multiplication, and shoot induction from the meristem. The procedure that yielded a higher shoot regeneration rate with meristem-derived callus involved shoot multiplication with 1001 medium followed by the Caboni’s callus induction medium, then without callus propagation on liquid medium, and then either Caboni’s or Saito-Suzuki’s shoot induction medium. In this experiment, axillary buds may remain as the shoot apexes were excised at 1 mm. In the next experiment, shoot apexes were excised at 0.5 mm to completely eliminate the axillary bud. Then treatment in the dark was added to the procedure to further improve shoot regeneration rates for the meristem-derived callus. Shoot multiplication medium and shoot induction medium procedures were conducted under dark conditions. This yielded the following optimal procedure: excising meristems from chlorosis shoots after 2–3 months of dark treatment in shoot multiplication medium on 1001; placing the excised meristems in Carboni’s callus induction medium in the dark for 20 days; then transferring the formed callus Saito-Suzuki’s shoot induction medium with incubation in the dark for at least 2 weeks. The shoot regeneration rates of calli treated in the dark for 6 weeks with shoot induction medium reached 68%. Relative to previous reports, this value is considered high for shoot regeneration from calli in apple cultivars.

著者

Xin LU Xiang WANG Lin PANG Jiayi LIU Qinghai YANG Xingchen SONG

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE Transactions on Fundamentals of Electronics, Communications and Computer Sciences (ISSN:09168508)

巻号頁・発行日

vol.E104.A, no.11, pp.1629-1643, 2021-11-01 (Released:2021-11-01)

参考文献数

38

被引用文献数

2

---

Network Slicing (NS) is recognized as a key technology for the 5G network in providing tailored network services towards various types of verticals over a shared physical infrastructure. It offers the flexibility of on-demand provisioning of diverse services based on tenants' requirements in a dynamic environment. In this work, we focus on two important issues related to 5G Core slices: the deployment and the reconfiguration of 5G Core NSs. Firstly, for slice deployment, balancing the workloads of the underlying network is beneficial in mitigating resource fragmentation for accommodating the future unknown network slice requests. In this vein, we formulate a load-balancing oriented 5G Core NS deployment problem through an Integer Linear Program (ILP) formulation. Further, for slice reconfiguration, we propose a reactive strategy to accommodate a rejected NS request by reorganizing the already-deployed NSs. Typically, the NS deployment algorithm is reutilized with slacked physical resources to find out the congested part of the network, due to which the NS is rejected. Then, these congested physical nodes and links are reconfigured by migrating virtual network functions and virtual links, to re-balance the utilization of the whole physical network. To evaluate the performance of deployment and reconfiguration algorithms we proposed, extensive simulations have been conducted. The results show that our deployment algorithm performs better in resource balancing, hence achieves higher acceptance ratio by comparing to existing works. Moreover, our reconfiguration algorithm improves resource utilization by accommodating more NSs in a dynamic environment.