- 著者
- Yu Saito Yuto Koseki Bunpei Hatano Kazuaki Sato Susumu Okubo Hitoshi Ohta Tateaki Ogata
- 出版者
- 科学・技術研究会
- 雑誌
- 科学・技術研究 (ISSN:21864942)
- 巻号頁・発行日
- vol.7, no.1, pp.61-68, 2018 (Released:2018-07-05)
The spin-trapping ESR (ST-ESR) method, which observes unstable radicals as stable spin adducts using electron spin resonance (ESR), is an effective experimental method for evaluating chemical reactions involving radical reactions. For instance, reactive oxygen species (ROS) such as hydroxyl radicals and so on is trapped well by spin-trapping reagent like as DMPO. In this research, we considered the analytical conditions of generation and detection of singlet oxygen. All conditions were decided based on the viewpoints of high usability for the singlet oxygen scavenging/quenching evaluating method. In addition, all experimental conditions were examined with a solvent-independent measurement method. In this study, a nonaqueous solution is N,N-dimethylformamide (DMF), and an aqueous solution is phosphate buffer solution (PBS, 100 mM pH 7.4). In each solvent CDCl3 or D2O was added at a 10 vol.% ratio respectively. Because heavy solvent was previously reported as an extending lifetime of singlet oxygen. The experimental conditions of singlet oxygen were examined by two different generation/detection methods. Photosensitization method using organic dye and thermal decomposition method by naphthalene derivative endoperoxide were studied as generation method, and 4,4’-bis (1-p-carboxyphenyl-3-methyl-5-hydroxyl) pyrazole (DRD156) and 2,2,5,5-tetramethyl-3-pyrroline-carboxamide (TPC) were studied as detection reagent, respectively. As a result, DRD156 and thermolysis method was good combination for singlet oxygen generation and detection in aqueous solution (around neutral condition) and nonaqueous solution.
- 著者
- Kazuaki SATO Kazutaka KANAI Maiko OZAKI Takaaki KAGAWA Mizuki KITA Yohei YAMASHITA Noriaki NAGAI Kazuki TAJIMA
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.18-0723, (Released:2019-02-25)
- 被引用文献数
- 3
We investigated the effects of tyrosol (Tyr) on anterior chamber paracentesis (ACP)–induced anterior uveitis in beagle dogs, as determined by protein and prostaglandin E2 (PGE2) concentrations in the aqueous humor (AH). Tyr at a dose of 100 or 200 mg/kg or 2.2 mg/kg of carprofen as a positive control was administered orally twice daily from 2.5 days before paracentesis. The initial ACP was performed in one eye of individual dogs and 0.5 ml AH was aspirated. The secondary AH was collected 60 min later. Pretreatment with 200 mg/kg of Tyr and carprofen significantly decreased aqueous protein and PGE2 concentrations compared to the control group. Overall, these findings suggested that Tyr was useful for the management of canine anterior uveitis.