著者

Kazuho Yoshida Takahiro Yamashita Kengo Sasaki Keiichi Inoue Yoshinori Shichida Hideki Kandori

出版者

The Biophysical Society of Japan

雑誌

Biophysics and Physicobiology (ISSN:21894779)

巻号頁・発行日

vol.14, pp.183-190, 2017 (Released:2017-12-19)

参考文献数

44

被引用文献数

4

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We previously showed that the chimeric proteins of microbial rhodopsins, such as light-driven proton pump bacteriorhodopsin (BR) and Gloeobacter rhodopsin (GR) that contain cytoplasmic loops of bovine rhodopsin, are able to activate Gt protein upon light absorption. These facts suggest similar protein structural changes in both the light-driven proton pump and animal rhodopsin. Here we report two trials to engineer chimeric rhodopsins, one for the inserted loop, and another for the microbial rhodopsin template. For the former, we successfully activated Gs protein by light through the incorporation of the cytoplasmic loop of β2-adrenergic receptor (β2AR). For the latter, we did not observe any G-protein activation for the light-driven sodium pump from Indibacter alkaliphilus (IndiR2) or a light-driven chloride pump halorhodopsin from Natronomonas pharaonis (NpHR), whereas the light-driven proton pump GR showed light-dependent G-protein activation. This fact suggests that a helix opening motion is common to G protein coupled receptor (GPCR) and GR, but not to IndiR2 and NpHR. Light-induced difference FTIR spectroscopy revealed similar structural changes between WT and the third loop chimera for each light-driven pump. A helical structural perturbation, which was largest for GR, was further enhanced in the chimera. We conclude that similar structural dynamics that occur on the cytoplasmic side of GPCR are needed to design chimeric microbial rhodopsins.

著者

吉田 一穂 Kazuho Yoshida

雑誌

英米評論 = ENGLISH REVIEW (ISSN:09170200)

巻号頁・発行日

no.17, pp.127-142, 2002-12-20

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Many literatures, pictures, photographs of the Victorian age show that the contemporaries were interested in childhood, and the novels of Charles Dickens also show that he was interested in it. Dickens’s interest in childhood, was related to his experience in his own childhood ; John Dickens, Charles's father, was a cheerful person but he had no sense of economy. He was imprisoned in the Marshalsea prison, and Charles had to work at Warren’s Blacking warehouse, which gave him an agony and despair. Dickens's childhood experience in the Warren's Blacking warehouse made him feel that his own childhood had come to an abrupt end, and that he had been prematurely exposed to adult responsibilities and independence. Dickens had suffered from the trauma and expressed his view of childhood in his novels. Dickens represented the ill treatment of workhouse to children in Oliver Twist. The children suffered from the hunger. The poor relief and the New Poor Law of 1834 were the highly topical subjects when Dickens took them up in Oliver Twist, and his related sense of outrage at the misery of pauper children brought up in baby farms and adults living in workhouses remained strong right through to the end of his life. Oliver who says, “Please, sir, I want some more”, is treated like a criminal. Oliver barely escapes being apprenticed to a chimney sweeper. Dickens showed that the children of chimney sweeper were ill treated and connects such children to “the image of child coming home to heaven”, as William Blake (1757-1827) did in “The Chimney Sweeper”. Dickens expressed his feeling toward the children as victims of his age by ‘the image of children coming home to heaven’, relating Oliver's destiny to a workhouse, a chimney sweeper, an undertaker, and a criminal.

著者

吉田 一穂 Kazuho Yoshida 桃山学院大学兼任講師

出版者

桃山学院大学総合研究所

雑誌

英米評論 (ISSN:09170200)

巻号頁・発行日

no.18, pp.41-65, 2003-12

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In 1857, Charles Dickens (1812-70) revisited the Marshalsea prison to look back upon the past and make a necessary atmosphere in Little Dorrit (1857). The Marshalsea prison was the place which Dickens could not forget in his lifetime. Dickens returned to his father's experience of debt again while he was drawing the portrait of the Father of the Marshalsea, William Dorrit, as 'a very amiable and very helpless middle-aged gentleman.' John Dickens, Charles's father, was a cheerful person but he had no sense of economy. He was imprisoned in the Marshalsea prison, and Charles had to work at Warren's Blacking warehouse, which gave him an agony and despair. Dickens seems to change the relationship between his father and him into the relationship between William Dorrit and Amy in Little Dorrit. William Dorrit who is called 'the Father of the Marshalsea prison, is proud of the title although he is a prisoner for debt. Amy as a 'Little mother' of his father and the chief support of the family, shows consideration for her father ; she is a protector of her father and his respectability. Indelibly marked by the more than twenty years to which the Circumlocution Office has condemned William Dorrit behind those walls, it is forever impossible for him, even when he is released, to lose those psychological scars. In Book 2, Chapter 19, 'The Storming of the Castle in the Air', William returns to the identity in the Marshalsea prison. William Dorrit who lived for many years, there is a victim of social system. Arthur who becomes a prisoner of the Marshalsea prison in Book 2, Chapter 27, is also a victim of social system. Arthur who has invested in the business of Merdle, goes bankrupt after he killed himself. Arthur is a victim of Calvinism which drives people to the condition of confinement, and is a prisoner of the wicked religion of Mrs. Clennam. Dickens showed how Arthur could be released from the cultural ideology of Calvinism which made him an indecisive man and how he could get freedom. In Book 2, Chapter 29, Amy visits Arthur who went bankrupt and became the prison of the Marshalsea prison. Amy gives him motherly love. What has to be noticed is that Amy says to Mrs. Clennam, 'Be guided only by the healer afflicted and forlorn, the patient Master who shed tears of compassion for our infirmities', before the house of Mrs. Clennam collapses. The words of Amy show the forgiveness of sin as a theme of Little Dorrit. Moreover, the representation of nature emphasizes the relief by Jesus Christ just before the house of Mrs. Clennam collapses : "From a radiant centre over the whole length and breadth of the tranquil firmament, great shoots of light streamed among the early stars, like signs of the blessed later covenant of peace and hope that changed the crown of thorns into glory." Amy delivers Arthur from the ideology of Calvinism which Mrs. Clennam brought him. In Little Dorrit, Dickens attacked the Christianity of Mrs. Clennam which deprived Arthur of his liberty and imprisoned his mind. Mrs. Clennam adopts Arthur, the love child of Mrs. Clennam and his love, to raise him in righteousness and retribution, but her Christianity which justifies her scheme of retribution does not bring her and Arthur happiness. Dickens demonstrated that people could be released from vengeful feelings by a practice of forgiveness of sin as Jesus Christ had done, through showing how Arthur could be released from the influence of the vengeful thoughts of Mrs. Clennam with the help of Amy.