著者

Kazuya Yoshida

出版者

Japan Prosthodontic Society

雑誌

Journal of Prosthodontic Research (ISSN:18831958)

巻号頁・発行日

vol.62, no.2, pp.239-244, 2018 (Released:2018-05-08)

参考文献数

35

被引用文献数

31

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Purpose: Many patients with oromandibular dystonia, which is characterized by involuntary masticatory, lower facial, and/or tongue muscle contractions, experience relief of symptoms through sensory tricks such as eating chewing gum or candy. The aim of this study was to identify the factors influencing the effects of splints in patients with oromandibular dystonia.Methods: Occlusal splints were inserted in 128 patients (89 women, 39 men) with oromandibular dystonia (102 with jaw closing dystonia, 20 with lingual dystonia, 5 with jaw deviation dystonia, 4 with jaw opening dystonia, 3 with lip dystonia, and 2 with jaw protrusion dystonia). Patients who showed improvement with the use of splints and continued to wear them for at least 3 months were defined as responders. In contrast, patients who showed little or no effect and/or were unable to insert splints were defined as non-responders. Differences in demographic and clinical data were statistically compared between responders and non-responders.Results: Ninety-eight patients (76.6%) were responders (subjective improvement: 30.5%). Thirty patients (23.4%) were non-responders (subjective improvement: 7.2%). The responders were significantly older than the non-responders (53.8 years vs 47.0 years; p < 0.05). Patients with jaw closing dystonia showed the most favorable results. The proportion of patients with sensory tricks was significantly higher in responders than in non-responders (66.3% vs 26.7%; p < 0.05).Conclusions: The sensory trick splint is especially helpful for patients with jaw closing dystonia. It is useful, although partially effective, as an alternative therapy in patients for whom other therapies have been unsatisfactory.

著者

Eiji Takita Kazuya Yoshida Shigeru Hanano Atsuhiko Shinmyo Daisuke Shibata

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.4, pp.391-400, 2021-12-25 (Released:2021-12-25)

参考文献数

41

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Genetic modification in plants helps us to understand molecular mechanisms underlying on plant fitness and to improve profitable crops. However, in transgenic plants, the value of gene expression often varies among plant populations of distinct lines and among generations of identical individuals. This variation is caused by several reasons, such as differences in the chromosome position, repeated sequences, and copy number of the inserted transgene. Developing a state-of-art technology to avoid the variation of gene expression levels including gene silencing has been awaited. Here, we developed a novel binary plasmid (pTACAtg1) that is based on a transformation-competent artificial chromosome (TAC) vector, harboring long genomic DNA fragments on both sides of the cloning sites. As a case study, we cloned the cauliflower mosaic virus 35S promoter:β-glucuronidase (35S:GUS) gene cassettes into the pTACAtg1, and introduced it with long flanking sequences on the pTACAtg1 into the plants. In isolated transgenic plants, the copy number was reduced and the GUS expressions were detected more stably than those in the control plants carrying the insert without flanking regions. In our result, the reduced copy number of a transgene suppressed variation and silencing of its gene expression. The pTACAtg1 vector will be suitable for the production of stable transformants and for expression analyses of a transgene.

著者

Eiji Takita Kazuya Yoshida Shigeru Hanano Atsuhiko Shinmyo Daisuke Shibata

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.21.0823a, (Released:2021-10-26)

参考文献数

41

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Genetic modification in plants helps us to understand molecular mechanisms underlying on plant fitness and to improve profitable crops. However, in transgenic plants, the value of gene expression often varies among plant populations of distinct lines and among generations of identical individuals. This variation is caused by several reasons, such as differences in the chromosome position, repeated sequences, and copy number of the inserted transgene. Developing a state-of-art technology to avoid the variation of gene expression levels including gene silencing has been awaited. Here, we developed a novel binary plasmid (pTACAtg1) that is based on a transformation-competent artificial chromosome (TAC) vector, harboring long genomic DNA fragments on both sides of the cloning sites. As a case study, we cloned the cauliflower mosaic virus 35S promoter:β-glucuronidase (35S:GUS) gene cassettes into the pTACAtg1, and introduced it with long flanking sequences on the pTACAtg1 into the plants. In isolated transgenic plants, the copy number was reduced and the GUS expressions were detected more stably than those in the control plants carrying the insert without flanking regions. In our result, the reduced copy number of a transgene suppressed variation and silencing of its gene expression. The pTACAtg1 vector will be suitable for the production of stable transformants and for expression analyses of a transgene.

著者

Yudai YUGUCHI Warley F. R. RIBEIRO Kenji NAGAOKA Kazuya YOSHIDA

出版者

THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES

雑誌

TRANSACTIONS OF THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES, AEROSPACE TECHNOLOGY JAPAN (ISSN:18840485)

巻号頁・発行日

vol.14, no.ists30, pp.Pk_125-Pk_130, 2016 (Released:2017-02-08)

被引用文献数

1 5

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The surface of an asteroid features an irregular terrain and microgravity. Therefore, robotic exploration in an asteroid requires the adoption of an appropriate locomotion strategy. Moreover, an exploration robot is expected to be capable of moving to an area of scientific interest. In response to this, we have proposed a ground grip robot that moves by gripping the surface like a rock climber. By gripping the surface, the robot can prevent unintended flotation and rotation while propelling itself across the surface. When the idling arm is moved while the supporting arm's gripper is attached to the surface, all of the reaction forces act on the gripper. If the gripping force is to be exceeded, however, the robot could come adrift from the surface. In this paper, therefore, we propose a motion control method that does not act the reaction force on the gripper by utilizing the reaction null-space. Additionally, the tip position trajectory is generated as an ellipse to enable smooth movement and eliminate any frictional force. This control law was validated by a planar dynamic simulation. The simulation model assumes a dual-armed robot with three degrees of freedom (DOF) in each arm, while the uneven surface is simulated under certain conditions. As a result, the robot was able to move continuously with reactionless motion and the propriety of the control law was confirmed.

著者

Kazuya YOSHIDA S.L. IRISHI J. A. BOOSE

出版者

Parenteral Drug Association Japan Chapter

雑誌

日本PDA学術誌 GMPとバリデーション (ISSN:13444891)

巻号頁・発行日

vol.1, no.2, pp.71-76, 1999 (Released:2006-08-03)

参考文献数

7

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Viral spiking studies were used to validate the capacity of our manufacturing process of a biological product derived from animal origin to inactivate or eliminate potential viral contaminants. Since the present study was intended for early Phase I & II clinical trials, two model viruses, Xenotropic Murine Leukemia Virus and polio virus, type 1, which represent a range of physical, chemical, and biological properties were selected for examination. It is generally recommended to take at least two different viral inactivation removed procedures for the robustness of the manufacturing process. We therefore selected a heat treatment and an ethanol purification step, two steps which held the potential for inactivation through independent mechanisms (i.e., physical and chemical inactivation, respectively). Finally, with the design of the experiment taken into consideration, the manufacturing steps were scaled-down and validated. Further, based upon the data collected during validation, the step parameters used for the present study were considered worst-cases. The result of the present spiking study for the two steps was a combined reduction of > 11 logs for polio virus and > 8 logs for X-MuLV which was the maximum attainable clearance given the spiking titer and dilution level for both viruses and both manufacturing steps.

著者

Hiroshi Yasuda Toshie Yonashiro Kazuya Yoshida Tomiko Ishii Toyoharu Tsutsui

出版者

日本微量元素学会

雑誌

Biomedical Research on Trace Elements (ISSN:0916717X)

巻号頁・発行日

vol.16, no.4, pp.285-292, 2005 (Released:2006-07-19)

参考文献数

35

被引用文献数

4

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In order to investigate any role of minerals in autistic disorders, we measured 24 mineral contents in scalp hair samples of 360 autistic children and compared with those of healthy controls. In the male autistic group aged 4-9 years (N=200), the geometric means of many element levels, namely not only essential minerals such as cobalt, chromium, iodine, molybdenum and phosphorus, but also nickel, boron, cadmium and lead were significantly lower than those in the control group (p<0.001). In contrast, selenium level was significantly higher in the autistic group. On the mercury levels, no significant difference was observed between the two groups, though with a tendency of low concentration in the autistic group. Similar mineral imbalance profiles with a global mineral deficiency were observed in the other autistic groups aged 0-3 and 10-15 years (N=85 and 20) and also in the female autistic groups. In addition, high accumulation of a few elements such as iron, manganese, chromium, copper, sodium, aluminum, cadmium, lead, or mercury was observed in some autistic individuals. These findings indicate that autistic children are suffered from a global mineral deficiency in various trace elements, with some individuals being exposed to a marked accumulation of several elements. Autistic children may be classified to sub-groups, based on their mineral imbalance profile in hair.