- 著者
- Tetsuhiko Yasuno Koji Takahashi Kazuhiro Tada Hiroto Hiyamuta Maho Watanabe Kenji Ito Hisatomi Arima Kosuke Masutani
- 出版者
- The Japanese Society of Internal Medicine
- 雑誌
- Internal Medicine (ISSN:09182918)
- 巻号頁・発行日
- pp.1602-23, (Released:2023-06-21)
- 参考文献数
- 23
- 被引用文献数
- 1
Background The gut bacterial microbiota is altered in patients with chronic kidney disease (CKD). However, the bacterial composition at each stage of CKD is unclear in these patients, including those receiving renal replacement therapy. We herein report the changes in the gut microbiota among patients with CKD. Methods A total of 93 individuals were recruited for the study. Seventy-three patients had stage 3-5 CKD, including those receiving renal replacement therapy (CKD group), and 20 were age- and sex-matched controls (CKD stage 1-2). The gut microbiome composition was analyzed using a 16S ribosomal RNA gene-based sequencing protocol. Results At the genus level, the butyrate-producing bacteria Lachnospira, Blautia, Coprococcus, Anaerostipes, and Roseburia were more abundant in the control group (linear discriminant analysis score of >3) than in the CKD group. Lachnospira was more abundant in the control group than in patients with CKD stage 3a. Compared to the control group, multiplex butyrate-producing bacteria were deficient in patients with CKD stage 3b-5D, including in patients receiving renal replacement therapy. Conclusion Our findings highlight the fact that the gut bacterial composition, including butyrate-producing bacteria, deteriorates from CKD stage 3b. Even after renal replacement therapy, the bacterial composition did not change.
- 著者
- Kazuhiro Tada Kenji Ito Aki Hamauchi Koji Takahashi Renya Watanabe Ai Uchida Yasuhiro Abe Tetsuhiko Yasuno Katsuhisa Miyake Yoshie Sasatomi Hitoshi Nakashima
- 出版者
- 一般社団法人 日本内科学会
- 雑誌
- Internal Medicine (ISSN:09182918)
- 巻号頁・発行日
- vol.55, no.8, pp.969-973, 2016 (Released:2016-04-15)
- 参考文献数
- 26
- 被引用文献数
- 1 6
Clopidogrel was administered to a 67-year-old Japanese man to prevent the recurrence of cerebral infarction. Twelve weeks later, he was admitted to our hospital with acute renal failure, hemolytic anemia and thrombocytopenia, and was diagnosed with clopidogrel-induced thrombotic microangiopathy. Clopidogrel was immediately discontinued and corticosteroid and plasma exchange therapy were administered simultaneously. Thereafter, the patient's condition gradually improved. The patient had a decreased serum complement C3 level. This suggests that the activated alternative pathway is related to thrombotic microangiopathy (TMA). TMA is a critical drug-associated adverse reaction that clinicians should always be vigilant about, because clopidogrel is widely prescribed.
- 著者
- Ryoji Suzuki Shouji Houki Kenji Ito Hiroyuki Shibaike
- 出版者
- The Plankton Society of Japan, The Japanese Association of Benthology
- 雑誌
- Plankton and Benthos Research (ISSN:18808247)
- 巻号頁・発行日
- vol.18, no.4, pp.206-213, 2023-11-27 (Released:2023-11-30)
- 参考文献数
- 21
An environmental DNA (eDNA) detection technique using TaqMan qPCR and LAMP analysis was developed for the Asian clam Corbicula fluminea, which is causing alarm in Japan and worldwide as an invasive alien species. The qPCR primer and probe designed in this study specifically amplified C. fluminea DNA, but did not amplify C. sandai or C. japonica DNA, both native Japanese species. In contrast, LAMP analysis showed that C. sandai DNA also reacted, but with a delay after the amplification of C. fluminea DNA, and it was possible to specifically detect C. fluminea DNA by setting the LAMP analysis time to 30 min. Field surveys at three study sites with different habitat conditions for C. fluminea showed that eDNA detection using qPCR/LAMP analyses were consistent with C. fluminea distribution. The applicability of a simple filtration and concentration method for eDNA using glass fibers in suspension (SGF method) was verified, and the SGF method had a higher eDNA recovery capacity than the conventional aspiration method using glass fiber filter paper. eDNA extraction using the SGF method and eDNA analysis using qPCR/LAMP may be useful for qualitative and simple habitat estimation of C. fluminea in poor water quality environments.
- 著者
- Kenji Ito Akihiro Inoue Taeko Kimura
- 出版者
- The Plankton Society of Japan, The Japanese Association of Benthology
- 雑誌
- Plankton and Benthos Research (ISSN:18808247)
- 巻号頁・発行日
- vol.13, no.3, pp.104-115, 2018-08-27 (Released:2018-08-29)
- 参考文献数
- 45
- 被引用文献数
- 1 3
The study was conducted to identify the effect of different types of tube traps on the sampling efficacy and accuracy for invasive golden mussels, Limnoperna fortunei (Dunker, 1857), at four sites in Japan. The traps consisted of PVC tubes (diameter: 5.5 cm; length: 20 cm) inside which a PVC plate was fixed horizontally as settlement substrate, and we examined two trap characteristics: mesh covering at the tube ends (present or not) and substrate texture (even or uneven). Mesh covering had a negative effect (P<0.001) and uneven substrate had a marginally positive effect (P=0.08) on mussel settlement density. A positive relationship was found between larval and settlement density in traps without mesh. These results imply that the most effective type of tube trap had no mesh covering and used uneven substrate. The settlement density of the mussels was also examined on several surfaces of the tube trap and the rope used to suspend the trap. The mussel density on the rope was higher than that on the PVC plates and tubes. The best correlation between settlement density and larval density occurred on the outside of the PVC tubes.
- 著者
- Kenji Ito Hiroyuki Shibaike
- 出版者
- The Plankton Society of Japan, The Japanese Association of Benthology
- 雑誌
- Plankton and Benthos Research (ISSN:18808247)
- 巻号頁・発行日
- vol.16, no.2, pp.100-108, 2021-05-27 (Released:2021-06-11)
- 参考文献数
- 36
- 被引用文献数
- 8
The golden mussel Limnoperna fortunei (Dunker, 1857) is an invasive freshwater bivalve species that exerts harmful effects on the environment, as well as man-made structures, such as water-treatment systems. By using conventional sampling methods, it is difficult to detect mussels under low-density conditions; however, environmental DNA (eDNA) analysis may be a rapid and efficient method for monitoring this aquatic organism. In this study, we conducted surveys based on the eDNA analysis of L. fortunei in 15 farm ponds in Japan and compared the results with those of two conventional survey methods, visual census and plankton larval survey, to clarify the effectiveness of eDNA analysis for field surveys of L. fortunei. Primers and a probe specific to L. fortunei were developed, and a method for analysis was established. In the laboratory experiments, the species eDNA was detected in all water tanks containing the mussels, and the concentration of eDNA was high in the experimental tank that had high density of L. fortunei. In the field survey, L. fortunei eDNA was detected in all ponds where the mussels were found by conventional survey, and low concentrations of eDNA were also detected in several ponds where no L. fortunei were found by traditional methods. These results suggest that eDNA analysis has greater sensitivity for the detection of L. fortunei in farm ponds than that of conventional methods. Environmental DNA surveys have little impact on water management and are suitable for surveys at water facilities that have not yet been damaged by the mussels.
- 著者
- Nobuyuki IWAMA Yutaka GOTO Toshiyuki ARAKI Kenji ITO Ryoji YASUKURA Jun TSUCHIYA Jun KANEKO Kenji HASHIMOTO Katsuhiko NEDA Motoh KAWABATA
- 出版者
- The Association of Japanese Geographers
- 雑誌
- 地理学評論 (ISSN:13479555)
- 巻号頁・発行日
- vol.75, no.12, pp.767-771, 2002-10-01 (Released:2008-12-25)