著者

Hao ZHANG Zhong-Li LI Xiang-Zheng SU Li DING Ji LI Heng ZHU

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.17-0137, (Released:2018-03-08)

被引用文献数

4

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Rabbit mesenchymal stem cells (MSCs) are important seed cells in regenerative medicine research, particularly in translational research. In the current study, we showed that rabbit subchondral bone is a reliable source of MSCs. First, we harvested subchondral bone (SCB) from the rabbit knee-joint and initiated the MSC culture by cultivating enzyme-treated SCB. Adherent fibroblast-like cells that outgrew from SCB fulfill the common immuno-phenotypic criteria for defining MSCs, but with low contamination of CD45+ hematopoietic cells. Interestingly, differentiated SCB-MSCs expressed osteogenic and chondrogenic markers at significantly higher levels than those in bone marrow cell suspension-derived MSCs (BMS-MSCs) (P<0.05). No differences in the expression of adipogenic markers between SCB-MSC and BMS-MSC (P>0.05) were observed. Moreover, the results of the colony forming unit-fibroblast assay and sphere formation assay demonstrated that the SCB-MSCs had increased self-renewal potential. SCB-MSCs expressed higher levels of the stemness markers Nanog, OCT4, and Sox-2 compared to in BMS-MSCs (P<0.05). Furthermore, the results of both the CCK-8-based assay and CFSE dilution assay showed that SCB-MSCs exhibited enhanced proliferative capacity. In addition, SCB-MSCs exhibited higher phosphorylation of extracellular signal-related kinase/mitogen-activated protein kinase signaling, which is closely related to MSC proliferation. In conclusion, we identified SCB-MSCs as a novel stem cell population that met the requirements of MSCs; the unique properties of SCB-MSC are important for the potential treatment of tissue damage resulting from disease and trauma.

著者

Li DING Jing JIN Jianjun ZHOU

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE TRANSACTIONS on Fundamentals of Electronics, Communications and Computer Sciences (ISSN:09168508)

巻号頁・発行日

vol.E105-A, no.11, pp.1443-1449, 2022-11-01

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This brief presents A 16/32Gb/s dual-mode transmitter including a linearity calibration loop to maintain amplitude linearity of the SST driver. Linearity detection and corresponding master-slave power supply circuits are designed to implement the proposed architecture. The proposed transmitter is manufactured in a 22nm FD-SOI process. The linearity calibration loop reduces the peak INL errors of the transmitter by 50%, and the RLM rises from 92.4% to 98.5% when the transmitter is in PAM4 mode. The chip area of the transmitter is 0.067mm2, while the proposed linearity enhanced part is 0.05×0.02mm2 and the total power consumption is 64.6mW with a 1.1V power supply. The linearity calibration loop can be detached from the circuit without consuming extra power.

著者

森 哲 竹内 寛彦 森 直樹 Savitzky Alan H. Tang Yezhong Li Ding Tsai Tein-shun Nguyen Tao Thien Das Indraneil Silva Anslem de Mahaulpatha Dharshani 城野 哲平

出版者

京都大学

雑誌

基盤研究(C)

巻号頁・発行日

2014-04-01

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ヤマカガシは頸腺と呼ばれる特殊な防御器官を持ち、強い皮膚毒を持つヒキガエルを食べることによりその毒成分を取り込んでこの器官に溜める。また、特異的な防御ディスプレイを行って、その毒を効果的に捕食回避に利用する。本研究では、合計18種のユウダ亜科のヘビが同様の防御器官を持つことを確認し、それらの形態は多様化していることを明らかにした。また、分子系統解析の結果、頸腺システムは1回だけ進化してきたことを示した。さらに、ミミズ食に移行した一部のヘビでは、頸腺毒をヒキガエルではなくホタルから取り入れている可能性が示された。