著者

Da HU Li ZHANG Rong JIANG Cuiting LIAO Juanjuan XU Shifang JIANG Yongqiang YANG Ling LIN Jiayi HUANG Yi SHEN Li TANG Longjiang LI

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.67, no.3, pp.145-152, 2021-06-30 (Released:2021-06-30)

参考文献数

33

被引用文献数

9

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Acetaminophen (N-acetyl-p-aminophenol, APAP) overdose causes hepatotoxicity, even liver failure, and oxidative stress plays pivotal role in its pathogenesis. Nicotinic acid (NA) is one form of vitamin B3, which has been used to treat a series of diseases in clinic for decades. To date, several studies have evidenced that NA has anti-oxidative property. Therefore, NA may have the hepatoprotective potential against APAP-induced toxicity. Here, our aim was to investigate the beneficial effect of NA against hepatotoxicity induced by APAP and its mechanism in vivo. BALB/c mice were intraperitoneally injected with NA (100 mg/kg) 3 times at 24, 12 and 1 h before APAP (600 mg/kg or 400 mg/kg) challenge. The results showed that pretreatment of NA markedly improved the survival rate, alleviated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and mitigated the histopathological injuries compared to APAP-exposed mice. Furthermore, NA significantly elevated the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) content, while reduced malondialdehyde (MDA) level. Finally, the signaling pathway was probed. The western blot revealed that NA up-regulated Sirtuin1 (Sirt1), nuclear factor erythroid 2-related factor 2 (Nrf2) and NAD(P)H quinone dehydrogenase-1 (NQO-1) expression and down-regulated Kelch-like ECH-associated protein 1 (Keap1) level in liver followed APAP exposure, implying Sirt1/Nrf2 axis exerted an essential role in the protective mechanism of NA on APAP toxicity. In brief, pretreatment of NA effectively protects liver against hepatotoxicity due to overdose of APAP through an antioxidant dependent manner modulated by Sirt1/Nrf2 signaling pathway.

著者

Lijiao Fan Dongshan Wei Xingwang Yu Fengqiang Yu Jiameng Wang Guirong Sun Alatengsuhe Li Zhang Guosheng Zhang Haifeng Yang

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.73, no.2, pp.180-192, 2023 (Released:2023-06-06)

参考文献数

62

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To identify the function of the SpsNAC042 gene and its response to salt and drought stress, the SpsNAC042 gene was transformed into Populus hopeiensis by the Agrobacterium-mediated leaf disc method, and the phenotypic, physiological changes and related genes expression of transgenic lines were analyzed. The results showed that the number and length of roots of transgenic lines increased significantly. The leaves of transgenic lines curled inward. Under salt and simulated drought stress, the transgenic lines showed improved tolerance to salt and drought. The activities of SOD, POD, CAT and proline content in the transgenic lines were significantly increased, and the reduction rates of total chlorophyll and MDA content were significantly decreased, which indicated that the transgenic lines showed strong physiological responses under stress. Meanwhile, the gene expression of MPK6, SOS1, HKT1 and P5CS1 were significantly upregulated, and the gene expression of PRODH1 was significantly downregulated, which preliminarily verified the stress regulation mechanism that SpsNAC042 might activate. The above results showed that the SpsNAC042 gene could promote root development, make leaf morphology curl, and enhance P. hopeiensis tolerance to stress.

著者

Qingming Zhan Yuli Fan Yinghui Xiao Wanlu Ouyang Yuliang Lan Zhicheng Jin Jie Yin Li Zhang

出版者

International Community of Spatial Planning and Sustainable Development

雑誌

International Review for Spatial Planning and Sustainable Development (ISSN:21873666)

巻号頁・発行日

vol.6, no.4, pp.148-164, 2018-10-15 (Released:2018-10-15)

参考文献数

27

被引用文献数

1 5

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This paper introduces a comprehensive framework that assesses the urban heat environment and formulates urban wind paths. Compared with other ecosystems, the wind environment and heat environment in urban areas can be much more complicated and dynamic. Nonetheless, it is of great concern considering the agglomerated population and industries at stake. Hence, multiple computational techniques are developed to assess the contemporary heat environment, and to formulate feasible policies to improve it to a more liveable state by introducing the solution of natural wind. Three key factors are considered: solar radiation, which is the major source of heat; wind direction and wind speed, which transports heat in space; and urban land surface, which may affect radiation reflection, contain auxiliary heat sources or cause vertical air flow. Hence, mesoscale meteorological data are applied to provide information about solar radiation, and are used for simulating local wind flow; Landsat images can be translated into land surface temperature figures; and building and land use databases provide information about built-up features. These combined, the local heat environment in urban areas can be mapped and monitored in a periodic fashion, with wind path analysis providing possibilities in cooling down the hotspots. Practices in cities including Fuzhou and Wuhan have proved constructive, with some others still underway.

著者

Jing Xue Xiaorong Li Ping Liu Ke Li Liping Sha Xiaoli Yang Lili Zhu Zhen Wang Youping Dong Li Zhang Hong Lei Xiaoxia Zhang Xiaoying Dong Hao Wang

出版者

The Japan Endocrine Society

雑誌

Endocrine Journal (ISSN:09188959)

巻号頁・発行日

pp.EJ18-0567, (Released:2019-07-03)

被引用文献数

71

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Polycystic ovary syndrome (PCOS) represents an endocrine disorder, which is closely related with gut microbiota. Inulin, a kind of probiotics, has been proven to alleviate gut microbiota dysbiosis. Metformin, a biguanide agent, shows beneficial effects on chronic metabolic diseases. Our objective was to assess the effects and associated mechanisms of inulin and metforin on attenuation of PCOS in mice. Mice were divided into 4 groups: control group (CON), model group (MOD), inulin group (INU), metformin group (MET). The last three groups were fed 6 mg of dehydroepiandrosterone (DHEA) per 100 g body weight and 60% high-fat diet to generate mice model. After 21 days of intervention, mice were euthanized and associated indications were investigated. Body weight (BW) and testosterone (T) levels were significantly decreased, but estradiol (E2) levels were increased in INU or MET group, respectively. Ovary HE staining demonstrated that inulin or metformin ameliorated PCOS morphology. Inflammatory indicators from plasma and ovary including TNF-α, IL-6, and IL-17A were decreased in INU or MET group. Moreover, IL-10 in ovary of INU or MET group was increased. Sequencing and analysis of gut microbiota showed that compared to MOD group, Bifidobacterium was increased, but Proteobacteria, Helicobacter and Parasutterella were decreased in INU group. Helicobacter was decreased in MET group. Correlation analysis showed that gut microbiota was correlated with inflammatory factors. Our results revealed that inulin and metformin alleviated PCOS via anti-inflammation and modulating gut microbiota, which may contribute to potential clinical therapy for the disease.

著者

He Miao Jianjun Gao Zishuo Mou Baolei Wang Li Zhang Li Su Yantao Han Yepeng Luan

出版者

International Research and Cooperation Association for Bio & Socio-Sciences Advancement

雑誌

BioScience Trends (ISSN:18817815)

巻号頁・発行日

pp.2019.01055, (Released:2019-04-25)

参考文献数

31

被引用文献数

7

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Histone deacetylase is an important member of epigenetics and a well validated target for anti-cancer drug discovery. In this study, we designed and synthesized a series of twenty-one novel hydroxamic acid-based histone deacetylase inhibitors with 4-piperidin-4-yl-triazole as the core skeleton. Most target compounds displayed excellent inhibition rates toward histone deacetylases at the concentration of 1 μM. Among them, the inhibition rates of two compounds MH1-18 and MH1-21 exceeded 90%. Furthermore, these two compounds selectively inhibited the activity of histone deacetylase 6 with low IC50 values. The high potency of them toward histone deacetylase 6 was rationalized by molecular docking studies. We found that MH1-18 and MH1-21 moderately inhibited the proliferation of four human cancer cell lines SGC-7901, NCI-H226, MCF-7, and HL-60. However, MH1-21 showed potent efficacy in suppressing the migration of MCF-7 cells. Results obtained in the current study shed light on designing potent HDAC6 inhibitors as anti-cancer agents.

著者

Mei-Zhu CHI Lin ZHU Zeng-Li ZHANG Fang-Fang JIN Hao-Ran SHAO Jia-Yin ZHENG Chao WU Guang-Qiong HU

出版者

Center for Academic Publications Japan

雑誌

Journal of Nutritional Science and Vitaminology (ISSN:03014800)

巻号頁・発行日

vol.64, no.2, pp.161-167, 2018 (Released:2018-04-30)

参考文献数

42

被引用文献数

10

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This study was designed to determine whether there is a relationship between serum vitamin D levels and neurodevelopment and anthropometry in Chinese infants. A prospective cohort study with 160 women who gave birth to 160 healthy full-term infants and who were followed up for 6 mo was done. It included 80 pregnant women with vitamin D deficiency, and the other 80 pregnant women were enrolled matching the age and delivery method with a 25(OH)D level of more than 50 nmol/L. There was a signicant intergroup difference in length, weight or head circumference at birth (p<0.05). Meanwhile, there was a signicant intergroup difference in cognitive development and achievement at 6 mo (p<0.001). In multivariate analyses, maternal 25(OH)D levels less than 50 nmol/L were independently associated with a higher tendency for a low Bayley mental score (MDI) at 6 mo (OR=2.77, 95% CI: 1.44-5.35, p=0.002), as well as Bayley motor score (PDI) (OR=2.08, 95% CI: 1.07-4.04, p=0.032). Thus we observed that maternal vitamin D was associated with infant neurodevelopment and anthropometry.

著者

Yong LI Man-Li ZHANG Bin CONG Si-Ming WANG Mei DONG Françoise SAURIOL Chang-Hong HUO Qing-Wen SHI Yu-Cheng GU Hiromasa KIYOTA

出版者

(社)日本農芸化学会

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.75, no.8, pp.1554-1556, 2011-08-23 (Released:2011-08-23)

参考文献数

27

被引用文献数

27 13

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Achillinin A (2β,3β-epoxy-1α,4β,10α-trihydroxyguai-11(13)-en-12,6α-olide, 1), a new guaianolide isolated from the flower of Achillea millefolium, exhibited potential antiproliferative activity to A549, RERF-LC-kj and QG-90 cells with 50% inhibitory concentration (IC50) values of 5.8, 10 and 0.31 μM, respectively.