著者

Mariko OKAMOTO Takefumi SOEDA Ai ASAMURA Ko TANAKA Kyo WATANABE Teruo IKEDA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0418, (Released:2019-01-22)

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We previously showed that the promoter region of the human epidermal growth factor receptor (hEGFR) gene elicits high transduction efficiency, with transgene expression restricted to canine breast tumor cells. However, it was unclear whether this promoter induces tumor cell-specific transgene expression in canine urothelial carcinoma cells. Furthermore, compared with studies in human cancer cells, the utility of the telomerase reverse transcriptase (TERT) gene promoter for therapeutic transgene expression in canine cancer cells has not been evaluated thus far. Here, we compared the activity of these promoters in canine mammary tumor and urothelial carcinoma cells. Our results showed that compared with the TERT promoter, the hEGFR promoter was more useful as a tumor-specific promoter to induce efficient transgene expression in canine tumor cells.

著者

岡本 眞理子 Mariko Okamoto

出版者

日本福祉大学経済学会

雑誌

日本福祉大学経済論集 = The journal of economic studies (ISSN:09156011)

巻号頁・発行日

no.51, pp.1-12, 2015-09

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少額の融資をあつかうマイクロファイナンス機関は,発展途上国の農村住民,とりわけ女性達に金融へのアクセスを可能にし,金融インフラを実質的に提供してきた.しかし,その中には,メキシコのコンパルタモス銀行のように,実効年利子率が100%を超える高金利で無担保融資を行ってきたところがある.それらの金融機関の利子率はなぜそれほど高いのかについて,想定される諸要因を他のマイクロファイナンス機関と比較しつつ検討した.その結果,極めて小額であり短期貸し付けであることや,融資方法と地域特性のゆえに運営費が極めて高くなることが主たる要因であることを明らかにした.また,借り手にはなぜそれが受け入れられているのかについて,現実に支払われる利息額の小ささ故に事業を圧迫するものとはならないことを示し,また短期ローン利子率の年利換算が借り手にとっては重要ではない可能性を示唆した.

著者

Mariko OKAMOTO Atsushi MIURA Ryota ITO Toshiki KAMADA Yoichi MIZUKAMI Keiko KAWAMOTO

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.23-0054, (Released:2023-11-13)

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Helicobacter pylori is a well-known pathogen that causes chronic gastritis, leading to the development of gastric cancer. This bacterium has also been detected in dogs, and symptoms similar to those in humans have been reported. The cytotoxin-associated gene A (CagA) is involved in pathogenesis through aberrant activation of host signal transduction, including the nuclear factor-kappa B (NF-κB) pathway. We have previously shown the anti-inflammatory effect of the G-protein-coupled estrogen receptor (GPER) via inhibiting of NF-κB activation in several cells. Therefore, here, we investigated the effect of GPER on CagA-mediated NF-κB promoter activity and showed that CagA overexpression in gastric cancer cells activated the NF-κB reporter and induced interleukin 8 (il-8) expression, both of which were inhibited by the GPER agonist.

著者

Ikuyo OHASHI Kiyoko KATO Mariko OKAMOTO Sota KOBAYASHI Daisuke TAKAMATSU

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0531, (Released:2020-01-03)

被引用文献数

2 7

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Paenibacillus larvae and Melissococcus plutonius are bacterial pathogens of honey bee brood. As decontamination of beekeeping equipment, including combs, is essential to control these pathogens, we evaluated the disinfecting effects of slightly acidic hypochlorous acid water (SAHAW) and weakly acidified chlorous acid water (WACAW) on the pathogens. Both disinfectants exhibited strong disinfecting effects in suspension tests under no organic matter conditions and reduced both pathogens by >5 log10 CFU/ml. Although the microbicidal activity of SAHAW with an available chlorine concentration (ACC) of 10–30 ppm was decreased by organic matter, it reduced viable P. larvae spores in combs more efficiently than H2O when the comb was not as dirty. However, its efficacy on combs decreased at 4°C and when overused or highly contaminated combs were tested. WACAW with an ACC of ≥600 ppm had a higher disinfecting capacity than SAHAW, and efficiently removed P. larvae spores from combs even under organic matter-rich and low-temperature conditions. However, even by WACAW, the amount of viable spores in combs was not markedly reduced depending on contamination levels and P. larvae genotypes. These results suggest the usefulness of both disinfectants for decontaminating beekeeping equipment depending on the situations expected.

著者

岡本 眞理子 Mariko Okamoto

雑誌

日本福祉大学経済論集

巻号頁・発行日

vol.51, pp.1-12, 2015-09-30

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少額の融資をあつかうマイクロファイナンス機関は,発展途上国の農村住民,とりわけ女性達に金融へのアクセスを可能にし,金融インフラを実質的に提供してきた.しかし,その中には,メキシコのコンパルタモス銀行のように,実効年利子率が100%を超える高金利で無担保融資を行ってきたところがある.それらの金融機関の利子率はなぜそれほど高いのかについて,想定される諸要因を他のマイクロファイナンス機関と比較しつつ検討した.その結果,極めて小額であり短期貸し付けであることや,融資方法と地域特性のゆえに運営費が極めて高くなることが主たる要因であることを明らかにした.また,借り手にはなぜそれが受け入れられているのかについて,現実に支払われる利息額の小ささ故に事業を圧迫するものとはならないことを示し,また短期ローン利子率の年利換算が借り手にとっては重要ではない可能性を示唆した.