著者

Kenji Yukuhiro Kazuya Iwata Natuo Kômoto Shuichiro Tomita Masanobu Itoh Makoto Kiuchi

出版者

The Japanese Society of Sericultural Science

雑誌

Journal of Insect Biotechnology and Sericology (ISSN:13468073)

巻号頁・発行日

vol.81, no.1, pp.1_029-1_035, 2012 (Released:2013-01-28)

参考文献数

21

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We analyzed 3633 Japanese Bombyx mandarina COI sequences from 37 locations in Japan, found 94 segregating sites in 714-bp long COI nucleotide sequences and eventually identified 121 haplotypes. We further confirmed that the 121 haplotypes were different from the 8 B. mori haplotypes reported by Yukuhiro et al. (2011), and the result strongly suggested little gene flow from B. mori to Japanese B. mandarina. We discuss the properties of the mutational differences between the two species.

著者

Hideya Wakisaka Munetaka Nakagawa Keiko Wakisaka Masanobu Itoh

出版者

The Ornithological Society of Japan

雑誌

ORNITHOLOGICAL SCIENCE (ISSN:13470558)

巻号頁・発行日

vol.5, no.1, pp.133-137, 2006 (Released:2006-08-09)

参考文献数

19

被引用文献数

5

著者

Nobuyuki Inomata Manami Miyazaki Mayu Noguchi Masanobu Itoh

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.97, no.5, pp.221-227, 2022-10-01 (Released:2023-02-22)

参考文献数

30

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Physiological responses to environmental changes play important roles in adaptive evolution. In particular, homeostatic regulatory systems that maintain constant circulating glucose levels are crucial in animals. However, variation in circulating glucose levels and the genetic effects on phenotypic variation in natural populations remain to be clarified. Here, we investigated the hemolymph glucose levels in natural populations of Drosophila melanogaster and its sibling species, D. simulans, in Japan. We quantified hemolymph glucose concentrations in third instar larvae of 27 lines for each species, which were reared on either glucose-free or glucose-rich food. In both species, genetic variation was not a major component of phenotypic variation on either glucose-free or glucose-rich food. The hemolymph glucose concentrations were much higher in D. simulans than in D. melanogaster. Genetic variance was larger in D. simulans than in D. melanogaster. The observed differences between the two species may be associated with the much more recent colonization history of D. simulans populations in Japan and/or the tolerance to environmental stresses. Our findings suggest that natural selection acting on hemolymph glucose levels in D. melanogaster is different from that in D. simulans.

著者

Yasuko Kato Akiko Sawada Kazuki Tonai Hisashi Tatsuno Takahisa Uenoyama Masanobu Itoh

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.96, no.6, pp.259-269, 2021-12-01 (Released:2022-04-21)

参考文献数

55

被引用文献数

1

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A spontaneous mutation, enNK14, was a new allele of engrailed (en) in Drosophila melanogaster. Females of enNK14 have three spermathecae, instead of two in wild type, under a wide range of developmental temperatures, while the males show no abnormal phenotype. Spermathecae of the mutant female can accept inseminated sperms, albeit with a delay of at least an hour until full acceptance compared with wild type. The time course of decrease in the number of stored sperms was thoroughly similar between the mutant and wild type. enNK14 females produced fewer progeny than wild type females despite storing a larger number of sperms. The delay of sperm entry and lower fecundity suggested some functional defects in secretory products of the spermathecae. In addition, some spermathecae in the mutant were accompanied by a mass of brown pigments in the adipose tissue surrounding the capsule. Six contiguous amino acids, Ser340–Ala345, were replaced by one Thr in enNK14. In another mutant, enspt, Ser325 was also shown to be substituted by a Cys. These amino acid changes were located within a serine-rich region, in which Ser325, Ser340 and Thr341 were suggested as targets of Protein Kinase C by an in silico analysis. The splicing pattern of en mRNA did not differ between enNK14 and wild type in embryo, larva, pupa or adult. Our results suggest that en plays an important role in determining the number of spermathecae as well as in sperm storage function in the Drosophila female.