著者

Yasuko Kato Akiko Sawada Kazuki Tonai Hisashi Tatsuno Takahisa Uenoyama Masanobu Itoh

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.96, no.6, pp.259-269, 2021-12-01 (Released:2022-04-21)

参考文献数

55

---

A spontaneous mutation, enNK14, was a new allele of engrailed (en) in Drosophila melanogaster. Females of enNK14 have three spermathecae, instead of two in wild type, under a wide range of developmental temperatures, while the males show no abnormal phenotype. Spermathecae of the mutant female can accept inseminated sperms, albeit with a delay of at least an hour until full acceptance compared with wild type. The time course of decrease in the number of stored sperms was thoroughly similar between the mutant and wild type. enNK14 females produced fewer progeny than wild type females despite storing a larger number of sperms. The delay of sperm entry and lower fecundity suggested some functional defects in secretory products of the spermathecae. In addition, some spermathecae in the mutant were accompanied by a mass of brown pigments in the adipose tissue surrounding the capsule. Six contiguous amino acids, Ser340–Ala345, were replaced by one Thr in enNK14. In another mutant, enspt, Ser325 was also shown to be substituted by a Cys. These amino acid changes were located within a serine-rich region, in which Ser325, Ser340 and Thr341 were suggested as targets of Protein Kinase C by an in silico analysis. The splicing pattern of en mRNA did not differ between enNK14 and wild type in embryo, larva, pupa or adult. Our results suggest that en plays an important role in determining the number of spermathecae as well as in sperm storage function in the Drosophila female.

著者

鯵坂 学 徳田 剛 中村 圭 加藤 泰子 田中 志敬 Manabu Ajisaka Tsuyoshi Tokuda Kei Nakamura Yasuko Kato Yukitaka Tanaka

出版者

同志社大学社会学会

雑誌

評論・社会科学 = Hyoron Shakaikagaku (Social Science Review) (ISSN:02862840)

巻号頁・発行日

no.91, pp.1-87, 2010-05-31

---

日本の大都市では2000年を画期として都心回帰がみられる。その原因は、不況によりオフィス需要が減少し、大型マンションが建てられ、新住民の居住が促進されたためである。大阪市特有の地域住民組織「地域振興会」へのインタビューを通して、大阪市における新住民と旧住民との関係について調査した。その結果から、新住民のそれへの参加は少なく、旧住民中心の振興町会側も対応に苦慮し、新旧住民間の交流やコミュニティの形成が課題となっていることが判明した。

著者

Chikako YAMADA Hidehiko IZUMI Junko HIRANO Aya MIZUKUCHI Mitsuo KISE Tsukasa MATSUDA Yasuko KATO

出版者

(社)日本農芸化学会

雑誌

Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)

巻号頁・発行日

vol.69, no.10, pp.1877-1883, 2005 (Released:2005-10-23)

参考文献数

28

被引用文献数

16

---

The effect of germination and subsequent heat-processing on the degradation of soluble proteins, including some allergenic proteins, in brown rice grains was investigated. The content of soluble proteins, including 14–16-kDa and 26-kDa allergens, in the germinated and processed brown rice grains (GPR) was much lower than that of non-germinated brown rice. These proteins in brown rice grains were also much lower after subsequent heat-processing during the manufacturing process. The protease activity of germinated brown rice (GR) was detected and increased 1.5 times after germination. The optimum pH values for degradation of the 26-kDa and 14–16-kDa allergens in the GR grains were 4 and between 5 and 7, respectively. These results suggest that the decrease in the soluble proteins and allergens was induced in part by proteolytic degradation. The presence of a detergent enhanced the proteolytic degradation of the soluble proteins, especially of the 26-kDa allergen, in the brown rice grains. The degradation of the 26-kDa allergen was weakly inhibited by NEM, suggesting cysteine protease(s) may have been involved in its degradation. These results suggest that the two abundant allergens were degraded in a different manner and probably by different proteases in the grains during germination.