- 著者
- Yuka Inada Naomi Oyama-Okubo Masumi Yamagishi
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.QH-066, (Released:2023-06-10)
Although a strong floral scent is typical of hybrid lilies, Asiatic hybrid lilies (Lilium spp.) have only a weak scent. Therefore, developing new cultivars with pleasant floral scents is an important objective in Asiatic hybrid lily breeding programs. Among the wild species contributing to Asiatic hybrid lily establishment, L. cernuum has a characteristic scent that the nose can perceive. We crossed Asiatic hybrid lily cultivars twice with L. cernuum to introduce floral scent traits into Asiatic hybrid lilies, and scent emission in the F1 plants and their progeny lines was evaluated instrumentally. Flowers of L. cernuum emitted benzenoids/phenylpropanoids (mainly 2-phenylethanol and benzaldehyde), monoterpenes (predominantly linalool and linalool oxides), and a fatty acid (iso-valeric acid). Lilium cernuum gave off unpleasant odors mainly because of iso-valeric acid emission. The F1 flowers also emitted these three chemical classes. Among the lines derived from the crosses between Asiatic hybrid lily cultivars and the F1 plant, lines that emitted all three chemical classes and lines that released one or two of the three chemical classes were segregated, and some lines emitted benzenoids/phenylpropanoids or terpenoids without emitting fatty acids. We successfully selected elite breeding lines that predominantly emitted pleasant scents. Therefore, this study demonstrated the introduction of pleasant scent production capability into Asiatic hybrid lilies using interspecific hybridization with L. cernuum.
- 著者
- Masumi Yamagishi
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.UTD-373, (Released:2022-05-25)
- 被引用文献数
- 2
The lily MYB12 gene, a positive regulator of anthocyanin biosynthesis, is targeted by microRNA828 (miR828). In bicolor tepals of Asiatic hybrid lilies with white lower halves and pigmented upper halves, accumulation levels of miR828 are higher in the lower halves than in the upper halves, and action of MYB12 is suppressed in the lower halves, resulting in bicolor tepal development. This is a newly identified mechanism of color pattern development in flowers. However, which wild species has donated the miR828-mediated bicolor tepal traits to these hybrid lilies is uncertain, and whether miR828-dependent pattern development occurs in species other than Lilium and is responsible for other types of color patterns is unknown. In this study, miR828 accumulation levels were compared between anthocyanin pigmented and unpigmented regions of flowers in lilies and other species. Lilium dauricum is among the parental wild species of Asiatic hybrid lilies. Lilium dauricum showed bicolor tepals, in which anthocyanins highly accumulated in the upper halves, and miR828 accumulation was more than 10 times higher in the lower halves than in the upper halves. Thus, the profile of miR828 accumulation was similar to that found in bicolor cultivars of Asiatic hybrid lilies. It is possible that the miR828-mediated bicolor tepal trait in Asiatic hybrid lilies is derived from L. dauricum. In L. cernuum var. album and an Oriental hybrid lily ‘Dizzy’, the suppression of MYB12 expression causes unpigmented tepals or tepal regions, but the unpigmented regions are spatially different from those in bicolor tepals of Asiatic hybrid lilies. MiR828 accumulation levels were similar between white and pink flowers of L. cernuum, and rather higher in pigmented regions than white regions of ‘Dizzy’ tepals, suggesting little involvement of miR828 in MYB12 expression suppression. MiR828 accumulation levels were evaluated in bicolor flowers of cherry sage, tulip, and Alstroemeria, but differences in miR828 accumulation were not detected between red and white petal/tepal regions, indicating that the mechanisms by which the bicolor flowers developed in these species are likely different from that occurring in Asiatic hybrid lilies and L. dauricum. Thus, the miR828/R2R3-MYB module is likely responsible for color only in lily flowers and only for the color pattern that consists of lower un-pigmented and upper pigmented regions.
- 著者
- Masumi Yamagishi Takashi Nakatsuka
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-057, (Released:2017-02-28)
- 被引用文献数
- 14
Genes encoding a MYB12 transcription factor, which regulates anthocyanin biosynthesis, are the key genes causing variations in the anthocyanin colors in lily flowers. However, the origin of the MYB12 in Asiatic hybrid lilies (Lilium spp.) is not completely known. In this study, we analyzed anthocyanin pigments in tepals of L. maculatum, L. lancifolium, L. callosum, L. leichtlinii, L. davidii, L. bulbiferum, and L. dauricum, and clarified that L. dauricum and L. bulbiferum accumulated a single anthocyanin, cyanidin 3-O-rutinoside, in entire tepals, although these wild species had orange-colored tepals. The sequencing of MYB12 genes revealed seven allelic sequences among ten L. dauricum plants and two allelic sequences in one L. bulbiferum plant. These MYB12 sequences were the same as or similar to the sequences isolated from pink Asiatic hybrid lily cultivars, indicating that L. dauricum and L. bulbiferum have contributed to the anthocyanin coloration of these cultivars.
- 著者
- Takehiro Suzuki Masumi Yamagishi
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.MI-089, (Released:2015-12-10)
- 被引用文献数
- 1 16
Lilium leichtlinii (2n = 2x = 24) is the most commercially cultivated edible lily in Japan, although viral and fungal diseases are severe problems. Triploid L. lancifolium (2n = 3x = 36), the bulbs of which are also edible, adapts well to the climate in Japan, and feral triploid L. lancifolium plants are often seen along roadsides and in croplands. Thus, triploid L. lancifolium is an important genetic resource for edible lilies, but it develops many bulbils (aerial bulbs) on leaf axils. Bulbil formation is undesirable for edible lilies because bulbils can compete for photosynthate with bulbs, which limits the use of triploid L. lancifolium in commercial production. Edible lily cultivars have been bred by intraspecific crosses within L. leichtlinii, although interspecific hybridization, which increases the level of genetic variation, is the major technique used to breed floricultural lily cultivars. In this study, interspecific-hybrid plants were developed by crossing triploid L. lancifolium and L. leichtlinii, and their characteristics, including bulbil formation ability, were evaluated. The crosses of triploid L. lancifolium × L. leichtlinii developed aneuploid plants, of which the chromosome number ranged from 26 to 34. Bulbil formation ability was continuously distributed in the F1 offspring, although 86% of F1 plants did not develop bulbils, indicating that many aneuploid plants without bulbils are developed by this cross combination. The plants harboring abnormal anthers were segregated in the hybrids. In addition, pollen germination in the F1 hybrids was lower than 20%, and 85% of the hybrids exhibited no pollen germination. However, abnormal anther morphology and low pollen fertility should not be major problems for edible lily production because bulbs are the main commercial output. These results indicate that interspecific hybridization between triploid L. lancifolium and L. leichtlinii has the potential to develop elite edible lily cultivars.