著者

Kohsyo Yamauchi Noboru Manabe Yoshiki Matsumoto Koh-en Yamauchi

出版者

Japan Poultry Science Association

雑誌

The Journal of Poultry Science (ISSN:13467395)

巻号頁・発行日

vol.51, no.3, pp.327-332, 2014-07-25 (Released:2014-07-25)

参考文献数

19

被引用文献数

7 7

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Effects of wood vinegar (WV) on red mites, safety test for chicks, and egg production performance were examined. In the counting of red mite numbers at cage knots using RGB color range of red mites by the image analyzer, the red mite were decreased after spraying (P<0.01). In a WV safety test for 8-day-old chicks, one ml of water, original WV, or WV diluted 500 and 1,000 times were tube-fed twice daily for 6 days. The rate of body weight gain was decreased in original WV group than that in the control (P<0.05), but it was not different in both WV dilution groups compared with that in the water-only group. In spraying one ml WV to the hen’s face twice per day for 8 days, the egg production performances of WV group did not changed so much compared with those of the control and initial day. These results suggest that the WV did not reduced production performance.From these results, WV could exterminate red mites, and did not reduce egg production, suggesting that WV is a useful natural substance to exterminate red mites without harmful effect.

著者

Yusuke HORI Takatoshi OZAKI Yoshimitsu YAMADA Teruaki TOZAKI Heui-Soo KIM Ayaka TAKIMOTO Maiko ENDO Noboru MANABE Miho INOUE-MURAYAMA Kazuo FUJITA

出版者

日本ウマ科学会

雑誌

Journal of Equine Science (ISSN:13403516)

巻号頁・発行日

vol.24, no.3, pp.31-36, 2013 (Released:2013-09-30)

参考文献数

15

被引用文献数

2 11

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Genetic polymorphisms in genes related to neurotransmitters or hormones affect personality or behavioral traits in many animal species including humans. In domestic animals, the allele frequency of such genes has been reported to be different among breeds and it may account for breed differences in behavior. In this study, we investigated breed differences in horses in the dopamine receptor D4 gene (DRD4), which has been reported to affect horse personality. We collected samples from seven horse breeds including those native to Japan and Korea, and compared the sequence of the DRD4 exon3 region among these breeds. We found that there were two types of polymorphisms (VNTR and SNPs) in the exon3 region, and some of them seemed to be breed-specific. In addition, we found that the allele frequency of G292A, reported to be associated with horse personality, differed greatly between native Japanese horses and Thoroughbred horses. The frequency of the A allele which is associated with low curiosity and high vigilance, was much lower in native Japanese horses (Hokkaido, 0.03; Taishu, 0.08) than in Thoroughbreds (0.62). This difference may account for breed differences in personality or behavioral traits. Further studies of the function of these polymorphisms and their effect on behavior are indicated.

著者

Noboru MANABE Akira MYOUMOTO Yoshihiro KIMURA Yuzuru IMAI Miki SUGIMOTO Hajime MIYAMOTO Kazuhiro SAKAMAKI Yoshinori OKAMURA Manabu FUKUMOTO

出版者

THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

vol.42, no.6, pp.j135-j141, 1996 (Released:2010-10-20)

被引用文献数

1 2

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哺乳類の卵母細胞は成熟する過程で選抜が行われて大部分の卵母細胞は選択的に死滅するが,この制御機構は未だ不明である.顕微鏡下に卵胞構成細胞を容易に分離調製できるブタ卵胞を材料として,二次卵胞から胞状卵胞に至る過程の卵胞退行における顆粒層細胞アポトーシスの役割とその制御について調べ,次のような結果を得た.はじめにTUNEL法および電顕法にて in situに卵胞退行にともなう顆粒層細胞アポトーシスの推移を観察した結果、内腔側の細胞から始まったアポトーシスは基底膜側に広がること,隣接する顆粒層細胞がアポトーシス小体を取り込むこと,および退行の進行した卵胞でも卵丘細胞にはアポトーシスが認められないことが分かった.なお,アポトーシスの開始は,ウシでは基底膜側から,齧歯類ではランダムであり,種属差があることも分かった.組織学的に見いだされた顆粒層細胞と卵丘細胞間の差異は生化学的に測定したエンドヌクレアーゼ活性の差異としても確認できた.すなわち,顆粒層細胞でのみ中性Ca2+/Mg2+-依存性エンドヌクレアーゼ活性の上昇を認めた.なお,ブタ顆粒層細胞アポトーシスには中性Ca2+-依存性,中性Mg2+-依存性および酸性カチオン非依存性エンドヌクレアーゼは関与しないことが分かった.ついで顆粒層細胞にアポトーシスを誘導できる単クローン抗体を作成し,これによって誘導される顆粒層細胞アポトーシスの進行がシステインプロテアーゼ阻害剤によって阻害されることをフローサイトメトリー法およびDNA電気泳動法にて確認した.顆粒層細胞では,アポトーシスシグナルの細胞内伝達にインターロイキン1β 転換酵素様プロテアーゼが関与していると考えられる.