著者
Attaya Kungsuwan 長島 裕二 野口 玉雄 志田 保夫 Sunee Suvapeepan Panthip Suwansakornkul 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.53, no.2, pp.261-266, 1987 (Released:2008-02-29)
参考文献数
14
被引用文献数
30 46

Further attempts were made to identify the toxins of the horseshoe crab Carcinoscorpius rotundicauda which causes sporadically food poisonings in Thailand. Toxic C. rotundicauda eggs were crushed and extracted with water repeatedly. After being defatted with dichloromethane, the combined extracts were ultrafiltered to cut off more than 1, 000 dalton-substances. Toxic fractions were obtained from the filtrate by column chromatography on Bio-Gel P-2 and Bio-Rex 70. Cellulose acetate strip electrophoresis, TLC, HPLC, and other instrumental analyses demon-strated that the horseshoe crab toxin was essentially composed of tetrodotoxin and related sub-stances, along with paralytic shellfish poisons as minor components.