著者
カングスワン アタヤ 野口 玉雄 荒川 修 清水 潮 塚本 久美子 志田 保夫 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.54, no.10, pp.1799-1802, 1988-10-25 (Released:2008-02-29)
参考文献数
11
被引用文献数
6 11

Attempts were made to detect tetrodotoxin (TTX)-producing bacteria in the Thai horseshoe crab Carcinoscorpius rotundicauda, one of TTX-containing marine invertebrates. Thirteen dom-inant bacteria were isolated from the intestinal contents of live horseshoe crab specimens, and were cultured in a 2 × ORI medium at 20°C for 24h. Bacterial cells of each strain were harvested, ultrasonicated and ultrafiltered, and TTX and anh-TTX fractions were separated from the filtrate by Bio-Gel P-2 column chromatography. HPLC, UV and GC-MS analyses showed that all out of the 13 strains isolated produced anh-TTX, and one strain produced TTX and anh-TTX. The strain which produced both toxins was identified as Vibrio alginolyticus on the basis of biochemical and other characters.
著者
Attaya Kungsuwan 長島 裕二 野口 玉雄 志田 保夫 Sunee Suvapeepan Panthip Suwansakornkul 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.53, no.2, pp.261-266, 1987 (Released:2008-02-29)
参考文献数
14
被引用文献数
30 46

Further attempts were made to identify the toxins of the horseshoe crab Carcinoscorpius rotundicauda which causes sporadically food poisonings in Thailand. Toxic C. rotundicauda eggs were crushed and extracted with water repeatedly. After being defatted with dichloromethane, the combined extracts were ultrafiltered to cut off more than 1, 000 dalton-substances. Toxic fractions were obtained from the filtrate by column chromatography on Bio-Gel P-2 and Bio-Rex 70. Cellulose acetate strip electrophoresis, TLC, HPLC, and other instrumental analyses demon-strated that the horseshoe crab toxin was essentially composed of tetrodotoxin and related sub-stances, along with paralytic shellfish poisons as minor components.