2 0 0 0 OA Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR
- 著者
- Kosuke Izumitsu Kanako Hatoh Takuya Sumita Yuki Kitade Atsushi Morita Chihiro Tanaka Abdul Gafur Akira Ohta Masataka Kawai Takashi Yamanaka Hitoshi Neda Yuko Ota
- 出版者
- The Mycological Society of Japan
- 雑誌
- Mycoscience (ISSN:13403540)
- 巻号頁・発行日
- vol.53, no.5, pp.396-401, 2012 (Released:2023-03-31)
- 被引用文献数
- 106
We have optimized a simple and rapid preparation procedure for mushroom DNA extraction from colonies on media or from fruiting bodies for PCR amplification. The protocol combines microwaving twice for 1 min, cooling for 10 min, and centrifuging for 5 min. By using this procedure, more than 100 samples of mushroom DNA can be prepared within 1 h. The DNA obtained can be used for (1) identifying mushroom species by PCR and subsequent sequencing, (2) amplifying low copy number genes (at least 2,000 bp), and (3) screening genetic transformants. This technique will contribute to the mycology of mushroom species.
1 0 0 0 OA Antibody response to equine coronavirus in horses inoculated with a bovine coronavirus vaccine
- 著者
- Manabu NEMOTO Toru KANNO Hiroshi BANNAI Koji TSUJIMURA Takashi YAMANAKA Hiroshi KOKADO
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.79, no.11, pp.1889-1891, 2017 (Released:2017-11-17)
- 参考文献数
- 17
- 被引用文献数
- 17
A vaccine for equine coronavirus (ECoV) is so far unavailable. Bovine coronavirus (BCoV) is antigenically related to ECoV; it is therefore possible that BCoV vaccine will induce antibodies against ECoV in horses. This study investigated antibody response to ECoV in horses inoculated with BCoV vaccine. Virus neutralization tests showed that antibody titers against ECoV increased in all six horses tested at 14 days post inoculation, although the antibody titers were lower against ECoV than against BCoV. This study showed that BCoV vaccine provides horses with antibodies against ECoV to some extent. It is unclear whether antibodies provided by BCoV vaccine are effective against ECoV, and therefore ECoV challenge studies are needed to evaluate efficacy of the vaccine in the future.
- 著者
- Daisuke MORIOKA Ichiro KITAYAMA Kosuke NAKANO Masato KITANO Takashi YAMANAKA Hideyo KOYAMA Takashi MORIMOTO Hideki SONOBE Noriyuki MIYAZAKI
- 出版者
- The Japanese Society for Experimental Mechanics
- 雑誌
- Advanced Experimental Mechanics (ISSN:21894752)
- 巻号頁・発行日
- vol.1, pp.269-276, 2016-08-31 (Released:2017-02-06)
- 参考文献数
- 10
- 被引用文献数
- 1
The purpose of this study was to quantitatively analyze the stress and deformation that occur in plastic ankle-foot orthoses (PAFOs) during walking after it is trimmed. First, PAFOs with incorporated sensors were individually prepared for 2 patients with hemiplegia. Mechanical data were then obtained while individuals wore the PAFOs during walking. Here we report the results of mechanical data and finite element analysis of trimmed plastic ankle-foot orthoses worn by two patients with left-hemiplegia. The results could provide prosthetists with some suggestions for forming orthoses.