著者

Hiroshi Fujii Satoshi Ohta Keisuke Nonaka Yuichi Katayose Toshimi Matsumoto Tomoko Endo Terutaka Yoshioka Mitsuo Omura Takehiko Shimada

出版者

日本育種学会

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

pp.16060, (Released:2016-10-15)

被引用文献数

18

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Satsuma mandarins (Citrus unshiu Marc.) are the predominant cultivated citrus variety in Japan. Clarification of its origin would prove valuable for citrus taxonomy and mandarin breeding programs; however, current information is limited. We applied genome-wide genotyping using a 384 citrus single nucleotide polymorphism (SNP) array and MARCO computer software to investigate the satsuma mandarin parentage. Genotyping data from 206 validated SNPs were obtained to evaluate 67 citrus varieties and lines. A total of five parent–offspring relationships were newly found by MARCO based on the 206 SNP genotypes, indicating that ‘Kishuu mikan’ type mandarins (Citrus kinokuni hort. ex Tanaka accession ‘Kishuu mikan’ and ‘Nanfengmiju’) and ‘Kunenbo’ type mandarins (Citrus nobilis Lour. var. kunip Tanaka accession ‘Kunenbo’ and ‘Bendiguangju’) are possible parents of the satsuma mandarin. Moreover, cleaved amplified polymorphic sequences analysis showed that the genotypes of four regions in chloroplast DNA of ‘Kishuu mikan’ type mandarins were identical to that of the satsuma mandarin. Considering the historical background, satsuma mandarins may therefore derive from an occasional cross between a ‘Kishuu mikan’ type mandarin seed parent (derivative or synonym of ‘Nanfengmiju’) and a ‘Kunenbo’ type mandarin pollen parent (derivative or synonym of ‘Bendiguangju’).

著者

Keisuke Nonaka Hiroshi Fujii Masayuki Kita Takehiko Shimada Tomoko Endo Terutaka Yoshioka Mitsuo Omura

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-026, (Released:2016-10-04)

被引用文献数

14

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To protect the rights of breeders of the major citrus cultivars developed under breeding programs by the national institute of Japan, we developed a method of cultivar identification based on cleaved amplified polymorphic sequence (CAPS) markers, and used it to evaluate their identity and parentage. We selected 19 CAPS markers that had a single-locus origin and moderate polymorphism, and used them to construct genotyping data for 59 citrus cultivars (including American accessions), local varieties, and selections. Of the 19 CAPS markers, 8 were sufficient to discriminate among all accessions except ‘Mato’ buntan (Citrus grandis Osbeck) and ‘Hirado’ buntan (Citrus grandis Osbeck). Among the 33 Japanese cultivars, the parentage of 30 agreed with that reported, but ‘Setoka’, ‘Southern Red’, and ‘Reikou’ had discrepancies at one or more loci. Using 15 to 18 CAPS markers to validate the putative parentage revealed that the seed parent of ‘Setoka’ was ‘KyEn No. 4’, not ‘Tsunonozomi’, and the pollen parent of ‘Southern Red’ was ‘Osceola’, not ponkan (C. reticulate Blanco). The seed parent of ‘Reikou’ remains unknown.

著者

Atsushi Imai Takeshi Kuniga Terutaka Yoshioka Keisuke Nonaka Nobuhito Mitani Hiroshi Fukamachi Naofumi Hiehata Masashi Yamamoto Takeshi Hayashi

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-013, (Released:2016-09-24)

被引用文献数

12

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We analyzed the pedigree records (1995–2010) of the Kuchinotsu Citrus Breeding Program (KCBP) at the National Institute of Fruit Tree Science (NIFTS) in Japan, abbreviated as NIFTS-KCBP, to reveal the genetic background and current status of inbreeding and genetic uniformity of the parental cultivars/genotypes and their F1 breeding progenies. The founding genotypes mostly used for crossing in NIFTS-KCBP were satsuma mandarin (Citrus unshiu Marcow.), sweet orange (C. sinensis [L.] Osbeck), king mandarin (C. nobilis Lour.), clementine (C. clementina hort. ex Tanaka), mediterranean mandarin (C. deliciosa Ten.), dancy tangerine (C. tangerina hort. ex Tanaka), and ponkan (C. reticulata Blanco). The intensive use of these seven genotypes and their progenies as crossed parents has led to a high degree of inbreeding in the breeding population. Moreover, these seven genotypes have dominated about 80% of the genetic composition of the breeding population. Although further studies are needed to reveal the influence of inbreeding and genetic uniformity on agronomically important traits, these data offer useful information for the selection of cross combinations and breeding strategies in the ongoing NIFTS citrus breeding program, Japan.