文献一覧: Tetsuya Mori (著者)

9 0 0 0 OA A multimodal metabolomics approach using imaging mass spectrometry and liquid chromatography-tandem mass spectrometry for spatially characterizing monoterpene indole alkaloids secreted from roots

著者: Ryo Nakabayashi Noriko Takeda-Kamiya Yutaka Yamada Tetsuya Mori Mai Uzaki Takashi Nirasawa Kiminori Toyooka Kazuki Saito
出版者: Japanese Society for Plant Biotechnology
雑誌: Plant Biotechnology (ISSN:13424580)
巻号頁・発行日: vol.38, no.3, pp.305-310, 2021-09-25 (Released:2021-09-25)
参考文献数: 27
被引用文献数: 7

Plants release specialized (secondary) metabolites from their roots to communicate with other organisms, including soil microorganisms. The spatial behavior of such metabolites around these roots can help us understand roles for the communication; however, currently, they are unclear because soil-based studies are complex. Here, we established a multimodal metabolomics approach using imaging mass spectrometry (IMS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to spatially assign metabolites under laboratory conditions using agar. In a case study using Catharanthus roseus, we showed that 58 nitrogen (N)-containing metabolites are released from the roots into the agar. For the metabolite assignment, we used 15N-labeled and non-labeled LC-MS/MS data, previously reported. Four metabolite ions were identified using authentic standard compounds as derived from monoterpene indole alkaloids (MIAs) such as ajmalicine, catharanthine, serpentine, and yohimbine. An alkaloid network analysis using dot products and spinglass methods characterized five clusters to which the 58 ions belong. The analysis clustered ions from the indolic skeleton-type MIAs to a cluster, suggesting that other communities may represent distinct metabolite groups. For future chemical assignments of the serpentine community, key fragmentation patterns were characterized using the 15N-labeled and non-labeled MS/MS spectra.

2022-07-02 14:16:33
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5 0 0 0 OA A multimodal metabolomics approach using imaging mass spectrometry and liquid chromatography-tandem mass spectrometry for spatially characterizing monoterpene indole alkaloids secreted from roots

著者: Ryo Nakabayashi Noriko Takeda-Kamiya Yutaka Yamada Tetsuya Mori Mai Uzaki Takashi Nirasawa Kiminori Toyooka Kazuki Saito
出版者: Japanese Society for Plant Biotechnology
雑誌: Plant Biotechnology (ISSN:13424580)
巻号頁・発行日: pp.21.0504a, (Released:2021-06-24)
参考文献数: 27
被引用文献数: 7

2021-06-24 19:39:12
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3 0 0 0 OA Spatial metabolomics using imaging mass spectrometry to identify the localization of asparaptine A in Asparagus officinalis

著者: Ryo Nakabayashi Kei Hashimoto Tetsuya Mori Kiminori Toyooka Hiroshi Sudo Kazuki Saito
出版者: Japanese Society for Plant Biotechnology
雑誌: Plant Biotechnology (ISSN:13424580)
巻号頁・発行日: vol.38, no.3, pp.311-315, 2021-09-25 (Released:2021-09-25)
参考文献数: 14
被引用文献数: 5

Spatial metabolomics uses imaging mass spectrometry (IMS) to localize metabolites within tissue section. Here, we performed matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance-IMS (MALDI-FTICR-IMS) to identify the localization of asparaptine A, a naturally occurring inhibitor of angiotensin-converting enzyme, in green spears of asparagus (Asparagus officinalis). Spatial metabolome data were acquired in an untargeted manner. Segmentation analysis using the data characterized tissue-type-dependent and independent distribution patterns in cross-sections of asparagus spears. Moreover, asparaptine A accumulated at high levels in developing lateral shoot tissues. Quantification of asparaptine A in lateral shoots using liquid chromatography-tandem mass spectrometry (LC-MS/MS) validated the IMS analysis. These results provide valuable information for understanding the function of asparaptine A in asparagus, and identify the lateral shoot as a potential region of interest for multiomics studies to examine gene-to-metabolite associations in the asparaptine A biosynthesis.

2022-01-25 07:08:55
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2 0 0 0 OA Producing the sulfur-containing metabolite asparaptine in Asparagus calluses and a suspension cell line

著者: Ryo Nakabayashi Tomoko Nishizawa Tetsuya Mori Hiroshi Sudo Isao Fujii Takashi Asano Kazuki Saito
出版者: Japanese Society for Plant Biotechnology
雑誌: Plant Biotechnology (ISSN:13424580)
巻号頁・発行日: vol.36, no.4, pp.265-267, 2019-12-25 (Released:2019-12-27)
参考文献数: 6
被引用文献数: 7

Asparaptine, a conjugate of L-arginine and asparagusic acid, was found in green asparagus (Asparagus officinalis) using ultrahigh-resolution metabolomics for sulfur-containing metabolites (S-metabolites), called S-omics. Asparaptine has been shown to inhibit the activity of angiotensin-converting enzyme. Larger amounts of this S-metabolite are therefore required for further analysis; however, there are limitations that asparagus is a perennial plant and its spears, wherein asparaptine accumulates, can be mainly harvested at the spring to summer season. In order to overcome these, we prepared a callus and suspension cell line from green asparagus. Untargeted metabolome analysis using liquid chromatography-tandem mass spectrometry was performed in the materials as well as spears and three calluses derived from wild type Asparagus. The analysis demonstrated that the amount of asparaptine in the callus derived from the green asparagus was more than the others per mg dry weight. The suspension cell line treated with methyljasmonate showed the induction of asparaptine, suggesting that the asparaptine production is modifiable under appropriate culture conditions. The described materials can be utilized for the production of asparaptine and in integrated metabolomics to study the biosynthesis of this S-metabolite, which is currently unknown.

2021-07-17 17:18:45
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2 0 0 0 OA Metabolome Analysis of Oryza sativa (Rice) Using Liquid Chromatography-Mass Spectrometry for Characterizing Organ Specificity of Flavonoids with Anti-inflammatory and Anti-oxidant Activity

著者: Zhigang Yang Ryo Nakabayashi Tetsuya Mori Satoshi Takamatsu Susumu Kitanaka Kazuki Saito
出版者: 公益社団法人日本薬学会
雑誌: Chemical and Pharmaceutical Bulletin (ISSN:00092363)
巻号頁・発行日: vol.64, no.7, pp.952-956, 2016-07-01 (Released:2016-07-01)
参考文献数: 34
被引用文献数: 17

Oryza sativa L. (rice) is an important staple crop across the world. In the previous study, we identified 36 specialized (secondary) metabolites including 28 flavonoids. In the present study, a metabolome analysis using liquid chromatography-mass spectrometry was conducted on the leaf, bran, and brown and polished rice grains to better understand the distribution of these metabolites. Principal component analysis using the metabolome data clearly characterized the accumulation patterns of the metabolites. Flavonoids, e.g., tricin, tricin 7-O-rutinoside, and tricin 7-O-β-D-glucopyranoside, were mainly present in the leaf and bran but not in the polished grain. In addition, anti-inflammatory and anti-oxidant activity of the metabolites were assayed in vitro. Tricin 4′-O-(erythro-β-guaiacylglyceryl)ether and isoscoparin 2″-O-(6‴-(E)-feruloyl)-glucopyranoside showed the strongest activity for inhibiting nitric oxide (NO) production and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging, respectively.

2016-08-22 13:42:21
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1 0 0 0 OA Assessing the ADC of Bone-marrow on Whole-body MR Images in Relation to the Fat-suppression Method and Fat Content

著者: Tetsuya Tsujikawa Akira Makino Hiroshi Oikawa Shota Ishida Tetsuya Mori Yasushi Kiyono Hirohiko Kimura Hidehiko Okazawa
出版者: Japanese Society for Magnetic Resonance in Medicine
雑誌: Magnetic Resonance in Medical Sciences (ISSN:13473182)
巻号頁・発行日: pp.mp.2020-0129, (Released:2021-02-09)
参考文献数: 22
被引用文献数: 2

Purpose: To compare apparent diffusion coefficients (ADCs) of bone marrow on diffusion-weighted imaging (DWI) between two fat-suppression techniques, and to evaluate the association between bone-marrow ADCs and the proton density fat fraction (PDFF).Methods: Seventy-seven patients underwent whole-body DWI with short-inversion time inversion-recovery (STIR) (DWISTIR) and/or STIR + selective water-excitation (spectral-spatial RF [SSRF]) (DWISTIR+SSRF). ADCs of lumbar vertebrae (L3 and L4) were compared between DWISTIR and DWISTIR+SSRF, and correlated with the PDFF.Results: Lumbar ADCs obtained by DWISTIR and DWISTIR+SSRF were significantly correlated (L3: r = 0.90, P < 0.0001, L4: r = 0.90, P < 0.0001). Lumbar ADCs (× 10-6 mm2/s) obtained by DWISTIR were significantly lower than those by DWISTIR+SSRF (L3: 479 ± 137 and 490 ± 148, P < 0.05, L4: 456 ± 114 and 471 ± 118, P < 0.005). Residual fat signals were more clearly observed on DWISTIR than on DWISTIR+SSRF. The ADCs of L3 obtained by DWISTIR and DWISTIR+SSRF exhibited significant positive correlations with the PDFF (r = 0.51, P < 0.0001, and r = 0.45, P < 0.0001, respectively), and the ADCs of L4 obtained by DWISTIR and DWISTIR+SSRF exhibited significantly positive correlations with the PDFF (r = 0.40, P < 0.0005, and r = 0.40, P < 0.0005, respectively).Conclusion: Irrespective of different fat-suppression methods, lumbar ADCs were positively correlated with the PDFF, being inconsistent with previous studies. Lumbar ADCs obtained by DWISTIR were significantly lower than those obtained by DWISTIR+SSRF, probably due to residual fat signals on DWISTIR. However, this difference (< 4%) did not explain the positive correlation between lumbar ADC and PDFF.

2021-02-09 12:58:38
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1 0 0 0 OA Characterization of the Volume-based or Number-based Size Distribution for Silica Nanoparticles Using a Unique Combination of Online Dynamic Light Scattering Having a Uni-tau Multi-bit Correlator and High-resolution Centrifugal Field-Flow Fractionation Separator

著者: Tetsuji YAMAGUCHI Tetsuya MORI Kengo AOKI Ryutaro ODA Masatoshi YASUTAKE Ayako NAKAMURA Kayori TAKAHASHI Tomoo SIGEHUZI Haruhisa KATO
出版者: The Japan Society for Analytical Chemistry
雑誌: Analytical Sciences (ISSN:09106340)
巻号頁・発行日: vol.36, no.6, pp.761-768, 2020-06-10 (Released:2020-06-10)
参考文献数: 15
被引用文献数: 2

This paper presents a study of the size distributions of colloidal nanoparticles using an online dynamic light scattering (DLS) unit with a uni-tau multi-bit correlator (UMC) combined with a centrifugal field-flow fractionation (CF3) separator. Conventionally, the FFF-UV-MALS system utilizing field-flow fractionation (FFF) combined with a UV detector and multi-angle light scattering instrument (MALS) could be used to obtain the particle size distribution of colloidal nanoparticles. Lately, DLS as a technique to measure the size distributions of colloid materials has become prevalent. However, the DLS instrument will practically measure only the large particles in a multi-modal particle mixture. Therefore, the CF3-DLS w/UMC system that was developed consisted of a CF3 unit connected to an online DLS instrument with UMC. The system could measure the volume- or number-based size distribution with highly quantitative and accurate histograms for multi-modal samples. The size distributions were validated with size distributions obtained by images of an atomic force microscope (AFM). Two types of colloidal silica nanoparticles with different distribution widths were used in this study.

2020-06-10 19:47:33
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1 0 0 0 OA Producing the sulfur-containing metabolite asparaptine in Asparagus calluses and a suspension cell line

著者: Ryo Nakabayashi Tomoko Nishizawa Tetsuya Mori Hiroshi Sudo Isao Fujii Takashi Asano Kazuki Saito
出版者: Japanese Society for Plant Cell and Molecular Biology
雑誌: Plant Biotechnology (ISSN:13424580)
巻号頁・発行日: pp.19.1002a, (Released:2019-12-18)
参考文献数: 6
被引用文献数: 7

2019-12-20 17:11:24
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