- 著者
- Akihiko Hiroguchi Kohei Nakamura Tomomichi Fujita
- 出版者
- Japanese Society for Plant Biotechnology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.39, no.1, pp.13-17, 2022-03-25 (Released:2022-03-25)
- 参考文献数
- 34
- 被引用文献数
- 1 2
Multicellular organisms regulate cell numbers and cell fate by using asymmetric cell division (ACD) and symmetric cell division (SCD) during their development and to adapt to unfavorable environmental conditions. A stem cell self-renews and generates differentiated cells. In plants, various types of cells are produced by ACD or SCD; however, the molecular mechanisms of ACD or SCD and the cell division mode switch are largely unknown. The moss Physcomitrium (Physcomitrella) patens is a suitable model to study plant stem cells due to its simple anatomy. Here, we report the cell division mode switch induced by abscisic acid (ABA) in P. patens. ABA is synthesized in response to abiotic stresses and induces round-shape cells, called brood cells, from cylindrical protonemal cells. Although two daughter cells with distinct sizes were produced by ACD in a protonemal stem cell on ABA-free media, the sizes of two daughter cells became similar with ABA treatment. Actin microfilaments were spatially localized on the apices of apical stem cells in protonemata on ABA-free media, but the polar accumulation was lost under the condition of ABA treatment. Moreover, ABA treatment conferred an identical cell fate to the daughter cells in terms of cell division activity. Collectively, the results indicate ABA may suppress the ACD characteristics but evoke SCD in cells. We also noticed that ABA-induced brood cells not only self-renewed but regenerated protonemal cells when ABA was removed from the media, suggesting that brood cells are novel stem cells that are induced by environmental signals in P. patens.
- 著者
- Kazuhiro Matsumoto Tomomichi Fujita Saki Sato
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-135, (Released:2018-02-22)
- 被引用文献数
- 8
The evaluation of storability for the type 2 red-fleshed apple ‘Kurenainoyume’ is essential to expand its consumption for table and processing use, as the cultivation area of this cultivar has extended year by year. There is also little available information whether cold storage affects the skin and flesh coloration of ‘Kurenainoyume’ (type 2 apples). In the present study, we evaluated the effect of 1-methylcyclopropene (1-MCP) treatment on the storability of bagged and non-bagged fruit for respective table and processing use over 3 years. Furthermore, we determined the maximum storage duration of both bagged and non-bagged fruit with 1-MCP. No change was observed in the soluble solid content during cold storage regardless of 1-MCP and bagging treatments. The malic acid content, flesh firmness, and skin and flesh coloration decreased with longer storage duration, but the decrease in these parameters was alleviated by 1-MCP treatment. Without 1-MCP, the storage duration of both bagged and non-bagged fruit to maintain flesh firmness and coloration at acceptable levels was about 90 days after harvest (DAH). With 1-MCP, bagged fruit for table use could be stored for about 180–210 DAH with no severe decrease in flesh firmness. Non-bagged fruit for processing use could also be stored for the same duration as bagged fruit, but the flesh firmness severely deteriorated. Although cooling treatment during the fruit growing stage improved flesh coloration, we found that cold storage did not promote this factor.
- 著者
- Kazuhiro Matsumoto Toru Kobayashi Tomoaki Kougo Tomomichi Fujita Saki Sato Takaya Moriguchi
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-120, (Released:2018-03-13)
- 被引用文献数
- 5
We investigated the effect of fruit bagging treatment using three kinds of paper bags differing in light permeability on the red coloration of skin, flesh, and core in type 2 red-fleshed ‘Kurenainoyume’ apples by comparison with type 1 ‘Goshogawara’ apples. Skin coloration of both ‘Goshogawara’ and ‘Kurenainoyume’ was affected by light and the effect was more marked in ‘Kurenainoyume’ than ‘Goshogawara’. In contrast, the effect of fruit bagging treatment on the red coloration of the core was limited in ‘Goshogawara’, while core coloration was not observed in ‘Kurenainoyume’ type 2 apples. Flesh coloration was observed even under dark conditions in both cultivars, and the light tended to enhance the flesh coloration to some extent in both cultivars. Since fruit bagging treatment is a prerequisite for ‘Kurenainoyume’ to prevent cork spot-like physiological disorder (CSPD) in the skin, we optimized the fruit bagging treatment conditions using a light impermeable double-layered paper bag (2-layer bag), considering both prevention of CSPD and the red coloration of ‘Kurenainoyume’ skin and flesh. Bag-removal at 25, 35, and 45 days before harvest (DBH), resulted in good skin and flesh coloration without CSPD incidence. Moreover, there was no significant difference in fresh weight, soluble solid, or malic acid contents compared with the non-bagging control. Therefore, we recommend bag-removal from 25 to 45 DBH for ‘Kurenainoyume’ as a practical cultivation technique.
- 著者
- Kazuhiro Matsumoto Tomomichi Fujita Saki Sato Takaya Moriguchi
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-130, (Released:2018-03-13)
- 被引用文献数
- 9
‘Kurenainoyume’ is a newly released type 2 red-fleshed apple with pinkish flesh color. In our previous study, we revealed the effects of light condition on red coloration of the skin, flesh, and core of ‘Kurenainoyume’ apple fruit. In this study, we investigated the effects of temperature, shading, defoliation, and crop load on red coloration of the flesh. Low temperature improved flesh coloration; apples subjected to cooling treatment at 5°C or 10°C showed higher a* values of the Lab color space than the control (without cooling treatment), in a temperature-dependent manner. Low temperature reduced the soluble solid content to a lesser extent in some years, whereas fresh weight, malic acid content, and flesh firmness were not affected. Thus, the importance of temperature for flesh coloration was confirmed, similar to its effect on skin coloration. When the trees were covered with cheesecloth with a 50% shading rate from August 1 (82 days after full bloom: DAFB) to October 28 (170 DAFB), flesh coloration was negatively affected, and fresh weight, soluble solid, and malic acid contents were reduced relative to the control treatment (without shading). We also investigated the effects of defoliation (one-seventh of the control) and crop load (two- or threefold the crop load of the normal case control) on flesh coloration. Both defoliation and over crop load also decreased a* values, but their effects on the parameters related to fruit quality were not clear. Because all the treatments of shading, defoliation, and crop load relate to the efficiency of photosynthesis and the distribution of photosynthetic assimilates, our results also demonstrated that photosynthetic assimilates (carbohydrates) were one of the crucial factors affecting flesh coloration.
- 著者
- Kazuhiro Matsumoto Toru Kobayashi Tomoaki Kougo Tomomichi Fujita Saki Sato Takaya Moriguchi
- 出版者
- 一般社団法人 園芸学会
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- pp.OKD-117, (Released:2017-11-08)
- 被引用文献数
- 13
‘Kurenainoyume’ is a new red-fleshed apple cultivar with a gradually increasing cultivation area. However, cork spot-like physiological disorder (CSPD) of the apple skin has become a serious problem over the years. Therefore, the development of strategies to reduce and prevent CSPD is strongly desired by farmers. To this end, we investigated the effectiveness of i) spraying calcium (Ca), boron (B), or both on the tree and ii) pre-harvest fruit bagging. Ca or B solutions or both did not decrease CSPD incidence. Furthermore, no relationship was detected between CSPD and the Ca/B content of fruit and leaves, demonstrating that the occurrence of CSPD might not be due to a deficiency in these elements. Pre-harvest fruit bagging reduced the development of CSPD depending on the light permeability of the paper bags used. Moreover, CSPD development was positively correlated with sunshine duration. Therefore, to prevent CSPD, fruit should be covered with light-impermeable paper bags at least from mid-July to late-September because the fruit covered for a shorter period developed CSPD. Thus, we propose that pre-harvest fruit bagging with light impermeable paper could be a useful and practical strategy to reduce or prevent CSPD in ‘Kurenainoyume’.