著者

Takehiro Ochi Ippei Nishiura Mitsuyoshi Tatsumi Yoshimi Hirano Kouichi Yahagi Yasuhiro Sakurai Yuji Sudo Hironari Koyama Yuichi Hagita Yoshikatsu Fujimoto Shinji Kitamura Hideki Hashimoto Tomoya Nakamura Asobi Yamada Masayoshi Tanimoto Noriko Nishina

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.62, no.4, pp.329-332, 2013 (Released:2013-10-31)

参考文献数

15

被引用文献数

1 12

---

Here, to determine the effects of transport stress on blood parameters in dogs, we investigated the changes in hematologic and serum chemical parameters in healthy beagle dogs transported from Beijing, China, to Osaka, Japan, to obtain the background data. Only the activity of serum alkaline phosphatase increased clearly upon arrival, a change attributed to transport stress, but the activity gradually reduced afterward. No marked changes in levels of other blood parameters were noted. Our findings here suggest that alkaline phosphatase is a useful tool for studying transport stress.

著者

Shizuko NAGAO Masanori KUGITA Daisuke YOSHIHARA Tamio YAMAGUCHI

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.5, pp.477-488, 2012 (Released:2012-10-25)

参考文献数

81

被引用文献数

13 62

---

Polycystic kidney disease (PKD) is a hereditary disorder with abnormal cellular proliferation, fluid accumulation in numerous cysts, remodeling of extracellular matrix, inflammation, and fibrosis in the kidney and liver. The two major types of PKD show autosomal dominant (ADPKD) or autosomal recessive inheritance (ARPKD). ADPKD is one of the most common genetic diseases, with an incidence of 1:500–1,000. Approximately 50% of patients with ADPKD develop end-stage renal disease (ESRD) by the age of 60. On the other hand, ARPKD is relatively rare, with an incidence of approximately 1:20,000–40,000. ARPKD is diagnosed early in life, often prenatally. The gene products responsible for ADPKD and ARPKD distribute in primary cilia and are thought to control intercellular Ca2+. Two types of animal model of PKD have been established: spontaneous hereditary models identified by the typical manifestations of PKD and gene-engineered models established by modification of human orthologous genes. Both types of animal models are used to study the mechanism of cystogenesis and efficacy of medical treatments. In PKD progression, critical roles of signaling pathways including MAPK, mTOR, and PPAR-γ have been discovered with these models. Therefore, experimental animal models are indispensable for investigating molecular mechanisms of PKD onset and progression as well as potential therapeutic treatments.

著者

Tohru KIMURA

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.45, no.1, pp.1-13, 1996 (Released:2003-12-23)

参考文献数

36

被引用文献数

5 10

著者

Sekiguchi Yukari Owada Junya Oishi Hisashi Katsumata Tokio Ikeda Kaori Kudo Takashi Takahashi Satoru

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.4, pp.445-451, 2012

被引用文献数

9 4

---

Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of β-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of β-cells. Furthermore, we were able to monitor fetal β-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri.

著者

Hiroshi Masuya

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.4, pp.365-373, 2012 (Released:2012-07-26)

参考文献数

37

---

A huge amount of experimental data from past studies has played a vital role in the development of new knowledge and technologies in biomedical science. The importance of computational technologies for the reuse of data, data integration, and knowledge discoveries has also increased, providing means of processing large amounts of data. In recent years, information technologies related to “ontologies” have played more significant roles in the standardization, integration, and knowledge representation of biomedical information. This review paper outlines the history of data integration in biomedical science and its recent trends in relation to the field of experimental animal science.

著者

Toyoyuki Takada Toshihiko Shiroishi

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.61, no.4, pp.375-388, 2012 (Released:2012-07-26)

参考文献数

68

被引用文献数

4 6 4

---

Mammalian quantitative traits that are observed at the whole-body level, such as body weight and length and blood biochemical parameters, are determined by the cooperative effects of multiple genetic and epigenetic factors as well as environmental factors. This complexity has hampered the genetic analysis of quantitative traits. To overcome this difficulty, we have established a full set of consomic mouse strains, also known as chromosome substitution strains, by replacing every chromosome of the classical inbred strain C57BL/6J with its counterpart from the Japanese wild-mouse-derived inbred strain MSM/Ms. The core components of the genomes of these two strains originated from different mouse subspecies. The inter-subspecific large-genome divergence and phenotypic differences between the two strains allowed the identification of genetic determinants for many quantitative traits by comprehensive phenotype screening. For some quantitative traits, the genetic determinants could be dissected into multiple chromosomes, thereby reflecting strain differences between C57BL/6J and MSM/Ms and their simple additive effects on the background of the consomic host strain. For other quantitative traits, the measured values of some consomic strains often far exceeded the range of the two parental strains, which suggests that nonadditive genetic interactions occur among multiple genes located on the substituted MSM/Ms chromosomes and the consomic host chromosomes. Thus, the inter-subspecific consomic strains are unique tools that can be used to identify both additive and nonadditive genetic effects on quantitative complex traits.