著者

サクニミット モラコット 稲月 一高 杉山 芳宏 八神 健一

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals

巻号頁・発行日

vol.37, no.3, pp.341-345, 1988

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マウス肝炎ウイルス (MHV) , イヌコロナウイルス (CCV) , Kilhamラットウイルス (KRV) およびイヌパルボウイルス (CPV) に対する消毒薬, 加熱, 紫外線の殺ウイルス効果を検討した。コロナウィルス (MHVおよびCCV) に対しては, ほとんどの消毒薬, 60℃, 15分の加熱で不活化ができたが, パルポウイルス (KRVおよびCPV) に対しては, ホルムアルデヒド, ヨードホール, 次亜塩素酸ナトリウム, 亜塩素酸ナトリウム以外に有効な消毒薬はなく, 80℃, 30分の加熱でも不活化できなかった。紫外線は, いずれのウイルスに対しても, 15分の照射で不活化できた。また, 同一ウイルス群に属するウイルスは各処置に対して同程度の反応を示し, ウイルス種, 株による差は認められなかった。

著者

田嶋 嘉雄

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.13, no.4, pp.133-138, 1964-10-30 (Released:2010-08-25)

参考文献数

22

著者

日本医学会・動物実験現状調査会

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.12, no.1, pp.27-35, 1963-01-30 (Released:2010-08-25)

著者

安東 洪次

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物彙報 (ISSN:00075124)

巻号頁・発行日

vol.5, no.6, pp.65-67, 1956-11-30 (Released:2010-08-25)

著者

石川 一郎 小松 稔 梶尾 彊

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.11, no.4, pp.180-186, 1962-10-30 (Released:2010-08-25)

著者

Takashi KURAMOTO Mayuko YOKOE Kayoko YAGASAKI Tatsuya KAWAGUCHI Kenta KUMAFUJI Tadao SERIKAWA

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.59, no.2, pp.147-155, 2010 (Released:2010-05-19)

参考文献数

33

被引用文献数

6 23

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To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (am), Black eye (Be), grey (g), Pearl (Pel), siamese (sia); 1 coat pattern, head spot (hs); 2 coat textures, Rex (Re), satin (sat); and an ear pinnae malformation, dumbo (dmbo). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): am to Chr 1, sia to Chr 1, sat to Chr 3, Re to Chr 7, g to Chr 8, dmbo to Chr 14, and hs to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for sia. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.

著者

環境調節実験委員会・小動物班

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.15, no.1, pp.17-41, 1966-01-30 (Released:2010-08-25)

参考文献数

45

著者

依田 八治 中山 一栄 遊佐 智栄 佐藤 重男 福田 三五郎 松本 幸蔵 中川 雅郎

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.25, no.1, pp.7-11, 1976 (Released:2010-08-25)

参考文献数

24

被引用文献数

1 3

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種々の動物におけるBordetella bronchisepticaの分布を知る目的で, 過去10年間にわたりマウス, ラット, スナネズミ, ゴールデンハムスター, モルモット, ウサギ, イヌ, ネコ, カニクイサルの呼吸器について本菌の検索を試みた。その結果, マウス, スナネズミ, ゴールデンハムスターはいずれも陰性であったが, 他の動物種からはすべて本菌が分離された。すなわち, ラットでは7/25 (28.0%) コロニーから菌が分離され, これらのコロニーにおける陽性個体の検出率は10.0~61.3% (平均50.0%) であった。モルモットでは22/60 (36.7%) コロニーが本菌に汚染し, 各コロニーにおける陽性個体の検出率は6.7~58.2% (平均18.3%) を示した。ウサギの場合は被検個体の由来によって検出率に大きな差がみられ, 一般農家から集めたウサギでは3.0%が陽性であったのに反して, 実験動物として比較的大きな規模で生産されたコロニーでは51.5~77.2%の高い陽性率を示した。イヌ, ネコは一般家庭で愛玩動物として飼育していたものを検査の対象にしたが, 前者では12/226 (5.3%) 匹, 後者では4/126 (3.2%) 匹より本菌が検出された。サルについては, 健康な動物の気管で3/48 (6.3%) 匹が陽性であったが, 肺病変を有するものについて病変部の培養を行ってみると16/39 (41.0%) 匹より本菌が分離された。

著者

松岡 佳子 鈴木 二郎

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.36, no.1, pp.83-86, 1987

被引用文献数

1

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スナネズミは, いろいろな分野で実験に用いられ, 大変有用な実験動物であるが, その繁殖, 発育に関する報告は少ない。筆者らは, 10年来, てんかん発作形質を選抜目標として, 近交系作出を試みている。その過程で得られた, 繁殖, 発育に関する成績は以下のとおりであった。1.平均寿命は, 雄26±14カ月, 雌27±13カ月であった。2.体重は, 16週齢でほぼ一定に達し, 雄で72±5.69, 雌で62±7.39であった。3.産仔数については, ばらつきがあるが, 平均4.8±1.7であった。4.分娩数は3~10月が多く, これらの時期が繁殖時期と考えられた。5.離乳率は, 約80%であった。6.世代別産仔数には大きな変動は見られなかった。<BR>本研究にあたり, 終始御協力下さった, 本研究所動物室, 岡崎守博, 赤坂佳幸の両氏ならびにタイプを打って下さった岩瀬真子さんに深謝する。

著者

芳賀 良一

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.7, no.3, pp.69-80, 1958-04-30 (Released:2010-08-25)

参考文献数

28

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Heretofore, studies on ochotona has been limited to the distribution of its habitat and their stored food. The main object of this project was to live-trap ochotona, acquire factual ecological in formations which were necessaay in order to develope ochotona as a new experimental animal.This study was started July, 1957, and is still being continued, and there are still many more problems to be studied concerning ecology and keeping ochotona alive in captivity, but following tare the summary of the results of the study up till now.Ochotona is about the size of a water rat, they are very tame. They are plant-eating animal and their fondness for dried plants seems to be the ircharacteristics.1. Distribution of Ochotona is limited to Mt. Daisetsu, central mountain range, and part of Hidaka range. Population is specially dense in Mt. Daisetsu towards Kitami district.2. Their terrain is mostly in rocky region. Temperature of the interstice is very low even in summer, about 12 to 14 degree centigrade. Their living habit does not seems to be influenced by the outside temperature, humidity nor the vegetation of the region.3. Life span assumed to be from one to three years. Cruising radius about 30 to 40 m. Once settled they will not move from their range.4. They live in the hole or interstice together but do not seem to be cooperative towards each other.5. They do not hibernate. During snowing season, they are active both above the snow and also through the tunnel dug under the snow.6. As far as food is concerned, Ochotona has been observed to eat almost any plant avaiable in the region. They store their winter food in autumn, but during summer, aside from eating fresh plants, they have the habit of temporarily storing and eating half-dried plants.7. There are two kinds of faeces. One, round and pellet like, is usually found at fixed place. The other, long and viscuous, defecated mostly during night on the rock where it is airy. Ochotona has the habit of eating the so called night faeces, and there is every reason to suppose that coprophagy provides the ochotona with various supplies of vitamin B.8. Breeding season assumed between May and July.9. Litter size of Ochotona is between 2 to 3. Cub is slightly furred with eyes closed. About 15 days after birth, they are quite active and independent and they grow rapidly. By the 40th day, it almost reaches its full adult size.10. The author has used the following feed for the captive ochotona: a) quartered apple (fresh) b) lespedeza (bicolor) fresh leaves and flowers and/or half dried leaves, c) wheatgerm meal with supplement of mineral, vitamin A. D., salt and calcium, d) water, or block of ice, 11. Growth rate of captive ochotona was favourable. Increase in growth rate of the young was higher than those of Lemming or the Tundra red back vole.12. Diseases so far found in captive ochotona are as follows: Pneumonia, mycosis, diet defficiency, septicaemia and fly larvae.I wish to express my appreciation to Mrs. Masu Nomura, Miss Michi Nomura of Oiso-machi, Kanagawa Pref., Dr. K. Suzuki of the Veterinary Section for The Institute of the Infectious Diseases for the help given to the author in keeping captive ochotona.

著者

Akiyoshi Ishikawa Keita Sakai Takehiro Maki Yuri Mizuno Kimie Niimi Yasuhiro Oda Eiki Takahashi

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.66, no.1, pp.51-60, 2017 (Released:2017-01-27)

参考文献数

33

被引用文献数

19

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To understand sleep mechanisms and develop treatments for sleep disorders, investigations using animal models are essential. The sleep architecture of rodents differs from that of diurnal mammals including humans and non-human primates. Sleep studies have been conducted in non-human primates; however, these sleep assessments were performed on animals placed in a restraint chair connected via the umbilical area to the recording apparatus. To avoid restraints, cables, and other stressful apparatuses and manipulations, telemetry systems have been developed. In the present study, sleep recordings in unrestrained cynomolgus monkeys (Macaca fascicularis) and common marmoset monkeys (Callithrix jacchus) were conducted to characterize normal sleep. For the analysis of sleep–wake rhythms in cynomolgus monkeys, telemetry electroencephalography (EEG), electromyography (EMG), and electrooculography (EOG) signals were used. For the analysis of sleep–wake rhythms in marmosets, telemetry EEG and EOG signals were used. Both monkey species showed monophasic sleep patterns during the dark phase. Although non-rapid eye movement (NREM) deep sleep showed higher levels at the beginning of the dark phase in cynomolgus monkeys, NREM deep sleep rarely occurred during the dark phase in marmosets. Our results indicate that the use of telemetry in non-human primate models is useful for sleep studies, and that the different NREM deep sleep activities between cynomolgus monkeys and common marmoset monkeys are useful to examine sleep functions.

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.66, no.Supplement, pp.S23-S25, 2017 (Released:2017-06-10)

著者

Seiya MIZUNO Saori IIJIMA Tomoko OKANO Noriko KAJIWARA Satoshi KUNITA Fumihiro SUGIYAMA Ken-ichi YAGAMI

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.60, no.2, pp.161-167, 2011 (Released:2011-04-21)

参考文献数

14

被引用文献数

5 18

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We found 6 spontaneous mutant mice with long pelage hair in our ICR breeding colony. The abnormal trait was restricted to long hair in these mice, which we named moja. They were fertile and showed the same growth and behavior as wild-type mice. To investigate the manner of the genetic inheritance of the moja allele, offspring were bred by mating the moja mice; all offspring had long pelage hair. Furthermore, we performed a reciprocal cross between moja mice and wild-type ICR mice with normal hair. All offspring exhibited normal hair suggesting an autosomal recessive inheritance of the trait. The moja/moja hair phenotype was maintained in skin grafted onto nude mice, suggesting that circulating or diffusible humoral factors regulating the hair cycle are not involved in the abnormal trait. The phenotype of moja/moja mice is similar to that of Fgf5-deficient mice. Therefore, we examined the expression of Fgf5 by RT-PCR in moja/moja mice. As expected, no Fgf5 expression was found in moja/moja mouse skin. PCR and DNA sequence analyses were performed to investigate the structure of the Fgf5 gene. We found a deletion of a 9.3-kb region in the Fgf5 gene including exon 3 and its 5’ and 3’ flanking sequences. Interestingly, the genomic deletion site showed insertion of a 498-bp early transposon element long terminal repeat. Taken together, these results suggest that the long hair mutation of moja/moja mice is caused by disruption of Fgf5 mediated by insertion of a retrotransposon.

著者

玄 柄和 金 明洙 李 哲浩 鄭 英吉 尹 元基 徐 俊教 元 茂虎 金 賢澤 河 貞姫 金 桓默 崔 良圭 呉 洋錫

出版者

公益社団法人 日本実験動物学会

雑誌

日本疾患モデル学会記録 (ISSN:09188991)

巻号頁・発行日

vol.15, pp.54-54, 1999

著者

Chihiro MORI Kazuhiro WADA

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

pp.15-0008, (Released:2015-04-24)

被引用文献数

8

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Like humans, songbirds are one of the few animal groups that learn vocalization. Vocal learning requires coordination of auditory input and vocal output using auditory feedback to guide one’s own vocalizations during a specific developmental stage known as the critical period. Songbirds are good animal models for understand the neural basis of vocal learning, a complex form of imitation, because they have many parallels to humans with regard to the features of vocal behavior and neural circuits dedicated to vocal learning. In this review, we will summarize the behavioral, neural, and genetic traits of birdsong. We will also discuss how studies of birdsong can help us understand how the development of neural circuits for vocal learning and production is driven by sensory input (auditory information) and motor output (vocalization).

著者

Masashi HASHIMOTO Masayuki FUNABA Seinosuke OHSHIMA Matanobu ABE

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.44, no.1, pp.23-28, 1995 (Released:2003-12-23)

参考文献数

29

被引用文献数

3 5

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This study was conducted to examine the relationship between dietary nitrogen (N)-corrected metabolizable energy (MEn) and dietary digestible energy (DE) in cats, in order to verify the reliability of the present metabolizable energy (ME) system for cats. Four adult female cats were fed diets containing four different levels of crude protein (CP) (24, 35, 49, and 62% as fed) 4 hours a day in a 4 × 4 Latin square design to determine energy-and N-balance. Dietary CP levels had hardly any effect on daily food intake, but acid-ether extract (AEE) intake tended to increase and carbohydrate (CHO) intake tended to decrease, in response to increases in dietary CP levels. Apparent CP and AEE digestibility did not change, regardless of the experimental diet. In contrast, CHO digestibility tended to diminish as dietary CP levels increased. Although the ratio of urinary energy (UE) to urinary N (UN) was higher in cats fed the lowest CP diet, it was still much lower than in other mammals. Regression between UE/digestible crude protein (DCP) and N-balance indicated that dietary ME at N-equilibrium (i.e., MEn) could be expressed as DE -0.47 × DCP. MEn could also be estimated as DE -0.62 × DCP by using the average ratio of UE/(UN × 6.25). Both DCP coefficients were much lower than in other mammals, including dogs and pigs, suggesting a unique form of N metabolism in cats. Because ME values applied to practical feline feed ingredients have been either estimated in pigs or calculated according to the equation, DE -1.25 × DCP, similar to the method used for dogs, the present ME values for cats are believed to have been underestimated.

著者

Kazuyuki MEKADA Kuniya ABE Ayumi MURAKAMI Satoe NAKAMURA Hatsumi NAKATA Kazuo MORIWAKI Yuichi OBATA Atsushi YOSHIKI

出版者

公益社団法人 日本実験動物学会

雑誌

Experimental Animals (ISSN:13411357)

巻号頁・発行日

vol.58, no.2, pp.141-149, 2009 (Released:2009-05-16)

参考文献数

33

被引用文献数

76 267

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The C57BL/6 mouse is the most well-known inbred mouse strain, and has been widely used as a genetic background for congenic and mutant mice. A number of C57BL/6 substrains have been derived from the C57BL/6 founder line and are reported to differ in several phenotypes. There are several major sources of C57BL/6 substrains for the biomedical research community. The importance of their genetic and phenotypic differences among substrains, however, has not yet been well recognized by biomedical researchers. Here, we report the result of screening of the functional deletion of the nicotinamide nucleotide transhydrogenase (Nnt) gene and 1,446 SNPs genotyping among seven C57BL/6 substrains from different sources, such as C57BL/6J, C57BL/6JJcl, C57BL/6JJmsSlc, C57BL/6NJcl, C57BL/6NCrlCrlj, C57BL/6NTac, and C57BL/6CrSlc. The deletion of exon 7-11 in the Nnt gene that was previously reported in C57BL/6J was also observed in other C57BL/6J substrains, indicating that this functional deletion probably occurred at an early stage in the establishment of C57BL/6J substrains. The genotyping of SNP loci clearly demonstrate genetic differences between C57BL/6J and C57BL/6N substrains at 11 loci. Besides, we found another SNP differing between C57BL/6J and other C57BL/6J substrains available from commercial breeders. No genetic difference was detected among C57BL/6N substrains. The C57BL/6CrSlc mouse, originally derived from the National Cancer Institute of the NIH was found to be the same as the C57BL/6N substrains by the SNP pattern. These data will be useful for accurate genetic monitoring of genetically engineered mice with the C57BL/6 background.

著者

藤原 弘 杉崎 速雄 渡辺 九郎

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.14, no.1, pp.23-27, 1965-01-30 (Released:2010-08-25)

参考文献数

8

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健康成熟ウィスター系ラット♂72匹をもちい外気温の高い夏季の直射日光下に暴露したときの致死体温の決定と, 直射日光を遮蔽した屋外における一昼夜の体温変化を測定した。1.直射日光下 (外気温30~35℃) に暴露したラットの致死体温は44℃であった。2.直射日光下一外気温29~33℃一の場合には全例死亡するにはいたらなかった。3.直射日光下 (外気温29.5~35.0℃) では43.6~44.0℃で死亡するものが一番多く33%で, ついで43.1~43.5℃, 42.6~43.O℃の閾値は25%の同率で, つぎに42.1~42.5℃の12.5%, 41.5~41.6℃の4.2%の分布であった。4.ラットを直射日光から遮蔽した屋外24時間放置時の体温変動は, 外気温の変動に平行するが, 平均体温38.8℃以上になることはなく, 死亡例はなかった。

著者

大丸 環姫 橋本 博雄 高山 敏 秋元 健

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.24, no.1, pp.19-23, 1975 (Released:2010-08-25)

参考文献数

14

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実験動物の血中T4濃度, TBCおよびそれらの日内変動について検討し, 次の成績を得た。1.サル, イヌ, ウサギ, ラットの血中T4濃度とTBCを測定したところ, T4濃度はラットが最も高く, ついでサル, ウサギ, イヌの順であった。TBCはサルが最も高く, イヌ, ウサギ, ラットの間には明らかな差が見られなかった。イヌとラットにおいて, 血中T4濃度, TBCの性差は検討したが, いずれにおいても明らかな性差は見られなかった。2.イヌの血中T4濃度とTBCの日内変動を検討したが, T4濃度は深夜から明け方に高く, 午前中低く, またTBCは午前中高く, 深夜に低い明瞭な日内変動が見られた。3.ラットの血中T4濃度とTBCの日内変動の検討の結果, いずれにも著明な日内変動は見られなかった。(本論文の要旨は日本実験動物研究会第8回研究発表会において発表した。)

出版者

公益社団法人 日本実験動物学会

雑誌

実験動物 (ISSN:00075124)

巻号頁・発行日

vol.9, no.2, pp.61-62, 1960-03-30 (Released:2010-08-25)

参考文献数

8