出版者

日本植物細胞分子生物学会

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.17, no.Supplement, pp.29-42, 2000 (Released:2010-04-30)

著者

Emi Ito Tomohiro Uemura Takashi Ueda Akihiko Nakano

出版者

日本植物細胞分子生物学会

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.16.0218a, (Released:2016-04-09)

参考文献数

41

被引用文献数

9

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In plant cells, the trans-Golgi network (TGN) is known to act as the early endocytic compartment, whereas RAB5-localizing multivesicular endosomes (MVEs) act as the later compartment. Land plants and certain green algal species possess plant-unique RAB5 homologs (ARA6/RABF1 in Arabidopsis thaliana) in addition to the orthologs of animal RAB5 (RHA1/RABF2a and ARA7/RABF2b in A. thaliana), and these two RAB5 members reside in substantially overlapping but different subpopulations of MVEs. Several studies indicate that the TGN and MVEs are closely related; however, the distribution of the two RAB5 groups in relation to the TGN remains elusive. Here, we quantitatively showed that ARA6 and ARA7 are closely associated with the TGN, and the subpopulation of ARA6 and ARA7 overlaps with the TGN in the root epidermal cells of A. thaliana.

著者

Tomonao Matsushita

出版者

日本植物細胞分子生物学会

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.28, no.5, pp.515-519, 2011-12-25 (Released:2012-02-20)

参考文献数

18

被引用文献数

4

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Transcriptional gene silencing (TGS) is a phenomenon by which transgenes that share homology in their promoter regions are inactivated. TGS is known to be reduced by using different promoters to drive the expression of each transgene. However, in order to perform a large-scale genetic screen in which overexpression lines are mutagenized by T-DNA tagging and therefore harbor two types of transgene, it is critical to develop a technique that consistently blocks TGS. Here, I report a versatile method that completely prevents TGS in transgenic Arabidopsis. A seedling morphology-based assay demonstrated that TGS could be significantly diminished by using different terminator sequences for each transgene. Furthermore, it was suggested that TGS might be reduced if the orientations of the two T-DNA sequences were reversed relative to each other. By combining these strategies, I showed that TGS was completely blocked in over 50,000 T1 plants. These findings present a thorough and versatile method to prevent TGS that is potentially applicable to various plant species and is expected to be used in diverse situations, from basic to applied research fields.

著者

Calvino Martin Kamada Hiroshi Mizoguchi Tsuyoshi

出版者

Japanese Society for Plant Cell and Molecular Biology

雑誌

Plant biotechnology (ISSN:13424580)

巻号頁・発行日

vol.22, no.3, pp.179-183, 2005-09-01

参考文献数

42

被引用文献数

9 4

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Plants in the genus <i>Arabidopsis</i> are facultative LD plants that flower much earlier under LD conditions than SD regimens, with the photoperiod (or LD) pathway contributing to floral acceleration. <i>LHY</i> and <i>CCA1</i> genes, among other factors, have central roles in the circadian clock of <i>Arabidopsis</i>, which plays a key role in measuring day length. <i>GI</i> gene mediates the circadian clock and floral activator genes, <i>CO</i> and <i>FT</i>, to control photoperiodic flowering. <i>GI</i> is required to set the peak phase of <i>CO</i> expression at the end of the light period under LD conditions, so that the <i>CO</i> protein is stabilized and activated by light to increase <i>FT</i> expression. However, recent studies have demonstrated that the role of SDs is not solely to switch off CO activity. For example, GI interacts with SPY, a negative regulator of the GA signal. The flowering times of <i>gi</i> mutants were still significantly later under SD conditions than LD regimes, which suggests that <i>GI</i> has a potential role in accelerating the start of flowering, even under SDs. Over-expression of either <i>FT</i> or <i>TSF</i> genes caused early flowering, and the acceleration of flowering was enhanced under SDs, suggesting that SDs have an additional role to that in the <i>LHY</i>/<i>CCA1</i>-<i>GI</i>-<i>CO</i>-<i>FT</i> pathway. In this short review, we discuss the hidden roles of SDs in controlling flowering based on recent studies of the molecular genetics of flowering time in <i>Arabidopsis</i>.

著者

Maki Ishiguro Tomonobu Hori Takuya Ishida Makoto Yoshida Koji Takabatake Satoshi Kaneko Kiyohiko Igarashi Masahiro Samejima

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.27, no.3, pp.273-281, 2010-06-25 (Released:2010-07-15)

参考文献数

26

被引用文献数

5 4

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The basidiomycete Flammulina velutipes is one of the most popular edible mushrooms in Japan, and has the ability to grow on cellulosic biomass as a carbon source. In this study, we have isolated two enzymes belonging to glycoside hydrolase (GH) family 7 (FvCel7A and FvCel7B) from the cellulose-grown culture of the fungus, and cloned cDNAs encoding these enzymes by utilizing a transcriptomic database of this fungus. Although both enzymes contain a catalytic domain belonging to GH family 7, only FvCel7A has the family 1 carbohydrate-binding module at the C-terminal. Sequence comparison indicated that FvCel7A and FvCel7B have a similar pattern of disulfide bonds and similar active site architecture to other fungal GH family 7 enzymes, but show small differences at loop regions covering the active site, which may affect the reactivity of cellulosic substrates.