著者

Aki FUJIWARA-IGARASHI Yuko GOTO-KOSHINO Hiroyuki MOCHIZUKI Shingo MAEDA Yasuhito FUJINO Koichi OHNO Hajime TSUJIMOTO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0351, (Released:2013-01-24)

被引用文献数

6 11 2

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The p16, p15 and p14 genes are widely known as tumor suppressor genes in human medicine. Although a large number of genetic and epigenetic aberrations in these genes have been reported in human malignancies, canine malignancies have not been well analyzed on the aberrations of these genes. In this study, the full-length complementary DNA (cDNA) of the canine p16 gene was cloned using the 5’ and 3’ rapid amplification of cDNA ends methods. Based on the sequence data, primers specific for p16, p15 and p14 were designed. Using these primers, the expression of p16, p15 and p14 mRNAs could be individually evaluated by reverse transcriptase polymerase chain reaction. Genomic aberrations were also examined using genomic polymerase chain reaction. Two of the six canine lymphoid tumor cell lines did not express detectable levels of p16, p15 and p14 mRNAs, and wide-ranging deletions in the p15-p14-p16 genomic locus were suspected. Wide-ranging deletions were also speculated in 2 of 14 dogs with T-cell lymphoid tumors. On the other hand, similar failure of amplification suggesting wide-ranging deletions were not observed in any of the 14 dogs with B-cell lymphoma. Deletion of the p15-p14-p16 genomic locus could be one of the molecular aberrations in canine lymphoid tumor cells.

著者

Hye-Sook CHANG Hiroaki KAMISHINA Keijiro MIZUKAMI Yasuyuki MOMOI Masaaki KATAYAMA Mohammad Mahbubur RAHMAN Mohammad Mejbah UDDIN Akira YABUKI Moeko KOHYAMA Osamu YAMATO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0451, (Released:2013-01-18)

被引用文献数

9 26

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Canine degenerative myelopathy is an adult-onset, progressive neurodegenerative disease that occurs in multiple dog breeds, particularly Pembroke Welsh Corgis. Recently, a degenerative myelopathy-associated mutation of the canine SOD1 gene was identified as c.118G>A (p.E40K). In the present study, genotyping assays using conventional and real-time PCR methods were developed, and a preliminary genotyping survey was performed on 122 randomly selected Pembroke Welsh Corgis without any degenerative myelopathy-related clinical signs to determine the current allele frequency in Japan. Both of the assays provided clear-cut genotyping. The survey demonstrated the frequencies of the G/G wild-type, G/A heterozygote and A/A homozygote to be 9.0, 42.6 and 48.4%, respectively, indicating that the prevalence of the mutant A allele (69.7%) in Pembroke Welsh Corgis is extremely high in Japan.

著者

Daisuke FUKUI Gen BANDO Koji FURUYA Masanori YAMAGUCHI Yuji NAKAOKA Masao KOSUGE Koichi MURATA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0231, (Released:2012-09-04)

被引用文献数

1 6

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An outbreak of encephalitozoonosis occurred in a rabbit colony at a zoo in Japan. Throughout the two years after the onset, all 42 rabbits were investigated clinically, pathologically and serologically for prevention and control of the disease. Eleven rabbits (11/42, 26.2%) showed clinical symptoms. Of 38 rabbits examined to detect specific antibodies against Encephalitozoon cuniculi, 71.1% (n=27) were found seropositive; 20 out of 30 clinically healthy rabbits (except for 8 clinical cases) were seropositive. The infection rate was 76.2% (32/42), including five pathologically diagnosed cases. The results of serological survey revealed that asymptomatic infection was widespread, even among clinically healthy rabbits. However, encephalitozoonosis was not found by pathological examination in any other species of animals kept in the same area within the zoo. Isolation and elimination of the rabbits with suspected infection based on the results of serological examination were carried out immediately; however, encephalitozoonosis continued to occur sporadically. Therefore, all the remaining rabbits were finally slaughtered. Then, the facility was closed, and all the equipment was disinfected. After a two-month interval, founder rabbits were introduced from encephalitozoonosis-free rabbitries for new colony formation. Since then, encephalitozoonosis has not been seen in any animals at the zoo. In this study, biosecurity countermeasures including staff education, epidemiological surveillance and application of an “all-out and all-in” system for rabbit colony establishment based on serological examination were successfully accomplished with regard to animal hygiene and public health for the eradication of E. cuniculi.

著者

Mohammad Zahangir ALAM Md. Golam YASIN Hirotomo KATO Tatsuya SAKURAI Ken KATAKURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0134, (Released:2012-08-10)

被引用文献数

3 17

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Although Phlebotomus argentipes as the only known vector of visceral leishmaniasis (VL) is zoophilic in nature, VL is considered to be anthroponotic in the Indian subcontinent. Peripheral blood samples from 85 stray dogs were examined for any molecular evidence of Leishmania infection in VL endemic areas of Bangladesh. Parasite DNA was detected in a blood sample from 1 of 85 (1.2%) stray dogs using ITS1-PCR, and PCR sequencing of the rRNA-ITS and cytochrome b gene confirmed that the parasitic DNA was Leishmania donovani. The results support the assumption that dogs are a probable animal reservoir for the Leishmania parasite in Bangladesh. It will be important to investigate the possible epidemiological role of dogs in domestic foci of VL endemic areas in Bangladesh.

著者

Lobna MKAOUAR Yoshifumi ENDO He Xi JUN Soo-Jung LEE Tassanee JAROENSONG Manabu MOCHIZUKI Kazuyuki UCHIDA Hiroyuki NAKAYAMA Nobuo SASAKI Takayuki NAKAGAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.74, no.6, pp.713-718, 2012 (Released:2012-07-04)

参考文献数

29

被引用文献数

2 5 1

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In this study, the nuclear expression of nuclear factor kappa B (NF-κB) in 48 tissues specimens from 25 canine spontaneous mammary gland tumor (MGT) patients was assessed by immunohistochemistry to compare their levels with clinical features, histological types, prognostic outcomes and proliferative activities, including the mitotic index (MI) and cylcinD1 expression. Twelve of eighteen (66.7%) malignant tumor tissues showed greater than 10% nuclear staining, while benign tumor and hyperplastic tissues showed less than 10% nuclear staining. Higher nuclear expression of NF-κB was positively correlated with larger tumor size, lymph node metastasis and higher MI; however, no correlation was observed with distant metastasis and cyclin D1 expression. Higher NF-κB nuclear expression correlated with shorter patient survival. These findings suggest that NF-κB is a useful prognostic factor for canine MGT patients.

著者

林 小市

出版者

公益社団法人 日本獣医学会

雑誌

中央獸醫學雑誌 (ISSN:18839088)

巻号頁・発行日

vol.45, no.9, pp.697-710, 1932-09-20 (Released:2008-10-24)

著者

原 元宣 清水 武彦 福山 正文 野村 靖夫 代田 欣二 宇根 ユミ 広田 昭彦 矢後 啓司 山田 宏 石原 実

出版者

公益社団法人 日本獣医学会

雑誌

日本獸醫學雜誌(The Japanese Journal of Veterinary Science) (ISSN:00215295)

巻号頁・発行日

vol.49, no.4, pp.645-649, 1987-08-15 (Released:2008-02-13)

参考文献数

13

被引用文献数

8 9

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茨城県取手市に飼育されていた犬が1985年3月27日, 激しい掻痒, 自己損傷を伴う症状を発見後に死亡, この地域ではオーエスキー病が1981年以来豚に流行していたことから本病が疑われた。剖検では病巣は検出されなかったが, 病理組織学的に延髄と頚部脊髄に著明な血管周囲の細胞浸潤, 神経膠症, ノイロノファギーを認めた。脳・脊髄の混合乳剤および肺・脾臓の混合乳剤を2頭のウサギに接種したところ, いずれも掻痒症を呈し, 4日後に死亡した。感染犬脊髄をPK-15細胞MA-104細胞に接種することにより細胞病原性を示すウイルスが分離され, 著明な円形化と巨細胞形成が認められ, ギムザ染色で核内封入体がみられた。感染PK-15細胞の電顕観察では, ヘルペスウイルス様粒子が観察され, 蛍光抗体法により抗オーエスキー病ウイルス抗体の結合が確認された。

著者

高畑 倉彦

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.4, no.12, pp.948-951, 1931-12-20 (Released:2008-10-24)

著者

Junko SUZUKI Yohei NISHIO Yuki KAMEO Yutaka TERADA Ryusei KUWATA Hiroshi SHIMODA Kazuo SUZUKI Ken MAEDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.77, no.11, pp.1457-1463, 2015 (Released:2015-12-01)

参考文献数

28

被引用文献数

3 18

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In 2007–2008, a canine distemper virus (CDV) epidemic occurred among wild animals in Wakayama Prefecture, Japan, and many mammals, including the wild boar and deer, were infected. In this study, CDV prevalence among wild animals was surveyed before and after the epidemic. At first, an enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase-conjugated protein A/G was established to detect CDV antibodies in many mammalian species. This established ELISA was available for testing dogs, raccoons and raccoon dogs as well as virus-neutralization test. Next, a serological survey of wild mammalians was conducted, and it was indicated that many wild mammalians, particularly raccoons, were infected with CDV during the epidemic, but few were infected before and after the epidemic. On the other hand, many raccoon dogs died during the epidemic, but CDV remained prevalent in the remaining population, and a small epidemic occurred in raccoon dogs in 2012–2013. These results indicated that the epidemic of 2007–2008 may have been intensified by transmission to raccoons.

著者

杉本 正篤

出版者

公益社団法人 日本獣医学会

雑誌

中央獸醫會雑誌 (ISSN:18839096)

巻号頁・発行日

vol.41, no.4, pp.365-371, 1928-04-20 (Released:2008-10-24)

参考文献数

7

著者

Su-Hyung LEE Du-Min GO Sang-Ho WOO Jee-Yong EUN Dae-Yong KIM

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.16-0373, (Released:2016-11-05)

被引用文献数

1

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A six-year-old castrated male Maltese dog presented to a private animal clinic with a mass on the dog’s lower lip without any other clinical signs. The mass (3 × 2 × 2 cm) was whitish and grossly well circumscribed, and a histopathological examination revealed that the mass was composed of normal cartilage tissue surrounded by fibrous connective tissues. Based on the gross findings, histopathology and anatomical location of the mass, the first diagnosis of a cartilaginous choristoma in a dog was made.

著者

吉川 元久 西村 眞二 上杉 茂

出版者

公益社団法人 日本獣医学会

雑誌

中央獸醫學雑誌 (ISSN:18839088)

巻号頁・発行日

vol.51, no.6, pp.535-543_1, 1938-06-20 (Released:2008-10-24)

著者

吉川 元久 西村 眞二 上杉 茂

出版者

公益社団法人 日本獣医学会

雑誌

中央獸醫學雑誌 (ISSN:18839088)

巻号頁・発行日

vol.49, no.7, pp.607-616_1, 1936-07-20 (Released:2008-10-24)

著者

Yuuto KAWASHIMA Hiroyasu TAKAHASHI Megumi SHIMOO Yukino TAMAMURA Yoshiharu ISHIKAWA Koichi KADOTA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.78, no.6, pp.1075-1078, 2016 (Released:2016-07-01)

参考文献数

21

被引用文献数

4

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A large subcutaneous mass, formed on the left lower jaw of a 4-month-old Japanese Black male calf, was partially excised for histological and bacteriological examinations. Antibiotic treatment resulted in a good prognosis. Bacteria isolated from the excised material were characterized by weak hemolysis and positive reactions for catalase and oxidase, and were 99% identical to Mannheimia granulomatis strains. The presence of the leukotoxin gene product was demonstrated by polymerase chain reaction amplification. Histological examination showed that the excised material was composed of dense fibrous connective tissue with sparsely distributed eosinophilic granulomas or abscesses. These foci frequently contained Splendore-Hoeppli material with rod-shaped Gram-negative bacteria. Except for the absence of lymphangitis and the presence of basophils and mast cells, the histology of this lesion resembled that of lechiguana associated with coinfection of M. granulomatis and Dermatobia hominis. Leukotoxin was demonstrated by immunohistochemistry within Splendore-Hoeppli material and was judged to be responsible for its formation.

著者

和田 保

出版者

公益社団法人 日本獣医学会

雑誌

中央獸醫會雑誌 (ISSN:18839096)

巻号頁・発行日

vol.33, no.5, pp.249-263, 1920 (Released:2008-10-24)

著者

Soon-Seek YOON Jae-Won BYUN Min-Jeong KIM You-Chan BAE Yeun-Kyung SHIN Sorah YOON Garam LEE Jae-Young SONG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.73, no.2, pp.275-277, 2011 (Released:2011-03-03)

参考文献数

10

被引用文献数

1 7

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Clinical grape poisoning in two dogs (a 1.6-year-old male Shih Tzu and a 5-year-old female Yorkshire Terrier) was described in the present study. Clinical signs included decreased urine output in the Shih Tzu and ataxia in the Yorkshire Terrier after grape ingestion. The Shih Tzu died 5 days post-grape ingestion, while the Yorkshire Terrier died 3 days post-grape ingestion. Erythematous serosae and mucosae, multifocal red small intestinal foci, and blood and grape seeds were identified in the intestinal lumen. Brownish-yellow crystals were bilaterally identified in the renal pelvis. The primary histological findings were acute tubular necrosis of the proximal convoluted tubules, severe necrosis, and mineralization in the renal cortical tubules. Blood urea nitrogen, creatinine, and alanine aminotransferase were increased in the dogs. Many Korean veterinary clinicians have suspected clinical grape poisoning. However, to our knowledge, grape poisoning has not been identified by pathologic and clinicopathologic basis until this writing in Korea. Education and knowledge about the risks of grape poisoning is necessary for the prevention of accidental exposures.

著者

Yuki NEMOTO Tomoya HARAGUCHI Takako SHIMOKAWA MIYAMA Kosuke KOBAYASHI Kaori HAMA Yosuke KUROGOUCHI Noriyuki FUJIKI Kenji BABA Masaru OKUDA Takuya MIZUNO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.7, pp.1146-1150, 2017 (Released:2017-07-07)

参考文献数

17

被引用文献数

9

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A 16-year-old spayed female American Shorthair cat was presented with lethargy, anorexia, and wamble. Physical and blood examination did not reveal any remarkable findings. Abdominal ultrasonography identified the presence of a localized anechoic structure with a thick wall in contact with the small intestine and adjacent to the liver. Ultrasound-guided fine-needle aspiration of the structure revealed fluid containing numerous cocci and neutrophils. Two days after antibiotic treatment, exploratory laparotomy was performed and the content of the structure was removed before multiple lavages. The pathological and bacteriological examination results supported a confirmatory diagnosis of pancreatic abscess due to Staphylococcus aureus infection, making this the first such report in a cat. The cat remained healthy thereafter with no disease recurrence.

著者

Shota KAWAKAMI Kazuhiko OCHIAI Yuiko KATO Masaki MICHISHITA Hinako HIRAMA Ryo OBARA Daigo AZAKAMI Masami WATANABE Toshinori OMI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.17-0362, (Released:2017-11-21)

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Gliomas are common intracranial neoplasias in dogs. However, the underlying pathogenic mechanisms remain unclear. In humans, isocitrate dehydrogenase 2 (IDH2) is often mutated in gliomas. Although almost human IDH2 mutations have been identified at the Arg172 codon, few studies have reported structural, functional, or mutational information for canine IDH2. In this study, we cloned the full-length canine IDH2 (cIDH2) cDNA and substituted wild type Arg174 (cIDH2 WT: corresponding to R172 of human IDH2) with Lys (cIDH2 R174K). The cIDH2 WT and R174K proteins were overexpressed in HeLa cells, and their presence was confirmed using an anti-human IDH2-WT mAb (clone: KrMab-3) and an anti-IDH2-R172K mAb (clone: KMab-1). The IDH2 activity between cIDH2 WT and cIDH2 R174K transfectants was compared by measuring the production of NADH and NADPH. NADPH production was lower for cIDH2 R174K than that for cIDH2 WT transfectants. Finally, we detected increased expression of hypoxia inducible factor-1 alpha (HIF-1α) in cIDH2 R174K transfectants. This indicates that mutations at R174 can potentially induce carcinogenesis in canine somatic cells.

著者

Kohei NAKATA Harumi MIURA Hiroki SAKAI Takashi MORI Sanae SHIBATA Hidetaka NISHIDA Sadatoshi MAEDA Hiroaki KAMISHINA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.6, pp.999-1002, 2017 (Released:2017-06-10)

参考文献数

14

被引用文献数

10

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A 7-year-old cat was referred with pelvic limb ataxia. Radiography and CT revealed bone resorption of the L1 vertebral arch, and myelography identified a compressive extradural lesion. The mass was surgically removed and histopathologically diagnosed as giant cell osteosarcoma. Three years later, the recurrent tumor resection and vertebral fixation were performed. Six months later, vertebrectomy was performed to radically excise the recurrent mass and a titanium spinal cage was placed. The cat is alive approximately 5 years after the first surgery. This case report describes vertebrectomy and vertebral body replacement as a radical treatment for feline vertebral osteosarcoma.

著者

Mun Keong KOK James K. CHAMBERS Atsushi DOHATA Kazuyuki UCHIDA Ryohei NISHIMURA Hiroyuki NAKAYAMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.6, pp.984-987, 2017 (Released:2017-06-10)

参考文献数

15

被引用文献数

4

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A 10-year-old mixed breed dog was presented with a 0.8 cm diameter mass below the left eye region. The mass was surgically removed and processed for histopathological examination. Microscopically, tumor cells proliferated in small lobules, nests and cords, and the tumor parenchyma was separated by desmoplastic stroma. Majority of the tumor cells were periodic acid-Schiff (PAS)-positive, and the desmoplastic stroma was densely collagenous and mucinous. Immunohistochemical results showed that the tumor cells were diffusely positive for cytokeratin 15, cytokeratin 19 and CD 34, while cytokeratin 8 reactivity was limited to the tumor cells proliferating in cords. Few tumor cells were positive for nestin. Based on the histopathological findings, the tumor was diagnosed as desmoplastic tricholemmoma.