著者

Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya

出版者

Elsevier B.V.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.131, no.5, pp.861-872, 2007-11-30

被引用文献数

207 15810

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Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.