著者

Takahashi Kazutoshi Yamanaka Shinya

出版者

Elsevier Inc.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.126, no.4, pp.663-676, 2006-08-25

被引用文献数

20074

---

This article is related to the Nobel Prize in Physiology or Medicine 2012

著者

Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya

出版者

Elsevier B.V.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.131, no.5, pp.861-872, 2007-11-30

被引用文献数

207 15810

---

Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

著者

Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya

出版者

Elsevier B.V.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.131, no.5, pp.861-872, 2007-11-30

被引用文献数

15810

---

Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

著者

Koyanagi-Aoi Michiyo Ohnuki Mari Takahashi Kazutoshi Okita Keisuke Noma Hisashi Sawamura Yuka Teramoto Ito Narita Megumi Sato Yoshiko Ichisaka Tomoko Amano Naoki Watanabe Akira Morizane Asuka Yamada Yasuhiro Sato Tosiya Takahashi Jun Yamanaka Shinya

出版者

National Academy of Sciences

雑誌

Proceedings of the National Academy of Sciences of the United States of America (ISSN:00278424)

巻号頁・発行日

2013-11-20

被引用文献数

190

---

大規模解析により品質の悪い多能性幹細胞の見分け方を開発. 京都大学プレスリリース. 2013-11-19.

著者

Yoshida Yoshinori Takahashi Kazutoshi Okita Keisuke Ichisaka Tomoko Yamanaka Shinya

出版者

Elsevier

雑誌

Cell Stem Cell (ISSN:19345909)

巻号頁・発行日

vol.5, no.3, pp.237-241, 2009-08

被引用文献数

4 616

---

低酸素濃度培養によるiPS細胞樹立効率の改善 -効率的な樹立方法開発に貢献する知見をCell Stem Cellに報告-. 京都大学プレスリリース. 2009-08-28. http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/090828_2.htm