著者
Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya
出版者
Elsevier B.V.
雑誌
Cell (ISSN:00928674)
巻号頁・発行日
vol.131, no.5, pp.861-872, 2007-11-30
被引用文献数
207 9217

Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.
著者
Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya
出版者
Elsevier B.V.
雑誌
Cell (ISSN:00928674)
巻号頁・発行日
vol.131, no.5, pp.861-872, 2007-11-30
被引用文献数
9217

Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.
著者
Yoshida Yoshinori Takahashi Kazutoshi Okita Keisuke Ichisaka Tomoko Yamanaka Shinya
出版者
Elsevier
雑誌
Cell Stem Cell (ISSN:19345909)
巻号頁・発行日
vol.5, no.3, pp.237-241, 2009-08
被引用文献数
4 402

低酸素濃度培養によるiPS細胞樹立効率の改善 -効率的な樹立方法開発に貢献する知見をCell Stem Cellに報告-. 京都大学プレスリリース. 2009-08-28. http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/090828_2.htm