著者

Takahashi Kazutoshi Yamanaka Shinya

出版者

Elsevier Inc.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.126, no.4, pp.663-676, 2006-08-25

被引用文献数

20074

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This article is related to the Nobel Prize in Physiology or Medicine 2012

著者

Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya

出版者

Elsevier B.V.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.131, no.5, pp.861-872, 2007-11-30

被引用文献数

207 15810

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Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

著者

Takahashi Kazutoshi Tanabe Koji Ohnuki Mari Narita Megumi Ichisaka Tomoko Tomoda Kiichiro Yamanaka Shinya

出版者

Elsevier B.V.

雑誌

Cell (ISSN:00928674)

巻号頁・発行日

vol.131, no.5, pp.861-872, 2007-11-30

被引用文献数

15810

---

Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

著者

Koyanagi-Aoi Michiyo Ohnuki Mari Takahashi Kazutoshi Okita Keisuke Noma Hisashi Sawamura Yuka Teramoto Ito Narita Megumi Sato Yoshiko Ichisaka Tomoko Amano Naoki Watanabe Akira Morizane Asuka Yamada Yasuhiro Sato Tosiya Takahashi Jun Yamanaka Shinya

出版者

National Academy of Sciences

雑誌

Proceedings of the National Academy of Sciences of the United States of America (ISSN:00278424)

巻号頁・発行日

2013-11-20

被引用文献数

190

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大規模解析により品質の悪い多能性幹細胞の見分け方を開発. 京都大学プレスリリース. 2013-11-19.

著者

Okita Keisuke Yamanaka Shinya

出版者

Elsevier Inc.

雑誌

Experimental cell research (ISSN:00144827)

巻号頁・発行日

vol.316, no.16, pp.2565-2570, 2010-10-01

被引用文献数

68

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Somatic cells can be reprogrammed into pluripotent stem cells by introducing a combination of several transcription factors. The induced pluripotent stem (iPS) cells from a patient's somatic cells could be useful source of cells for drug discovery and cell transplantation therapies. However, most human iPS cells are made by viral vectors, such as retrovirus and lentivirus, which integrate the reprogramming factors into host genomes and may increase the risk of tumor formation. Studies of the mechanisms underlying the reprogramming and establishment of non-integration methods contribute evidence to resolve the safety concerns associated with iPS cells. On the other hand, patient-specific iPS cells have already been established and used for recapitulating disease pathology.

著者

Parr Callum J C Katayama Shota Miki Kenji Kuang Yi Yoshida Yoshinori Morizane Asuka Takahashi Jun Yamanaka Shinya Saito Hirohide

出版者

Springer Nature

雑誌

Scientific reports (ISSN:20452322)

巻号頁・発行日

vol.6, 2016-09-09

被引用文献数

78

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iPS細胞を選択的に識別・分離・除去できるしくみを合成RNAを用いて構築. 京都大学プレスリリース. 2016-09-09.

著者

Nakagawa Masato Taniguchi Yukimasa Senda Sho Takizawa Nanako Ichisaka Tomoko Asano Kanako Morizane Asuka Doi Daisuke Takahashi Jun Nishizawa Masatoshi Yoshida Yoshinori Toyoda Taro Osafune Kenji Sekiguchi Kiyotoshi Yamanaka Shinya

出版者

Nature Publishing Group

雑誌

Scientific Reports (ISSN:20452322)

巻号頁・発行日

vol.4, 2014-01-08

被引用文献数

487

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細胞移植に適した新しいヒトiPS細胞の樹立・維持培養法を確立. 京都大学プレスリリース. 2014-01-08.

著者

Kondo Takayuki Funayama Misato Tsukita Kayoko Hotta Akitsu Yasuda Akimasa Nori Satoshi Kaneko Shinjiro Nakamura Masaya Takahashi Ryosuke Okano Hideyuki Yamanaka Shinya Inoue Haruhisa

出版者

Elsevier B.V.

雑誌

Stem Cell Reports (ISSN:22136711)

巻号頁・発行日

2014-06

被引用文献数

116

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ヒトiPS細胞由来のグリア系神経前駆細胞移植でALSモデルマウスの生存期間を延長. 京都大学プレスリリース. 2014-06-27.

著者

Ohta Sho Nishida Eisuke Yamanaka Shinya Yamamoto Takuya

出版者

Elsevier

雑誌

Cell Reports (ISSN:22111247)

巻号頁・発行日

pp.1-10, 2013-10-17

被引用文献数

52

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大規模遺伝子解析により体細胞の初期化過程でRNAスプライシングパターンが変化することを解明. 京都大学プレスリリース. 2013-10-18.

著者

Yoshida Yoshinori Takahashi Kazutoshi Okita Keisuke Ichisaka Tomoko Yamanaka Shinya

出版者

Elsevier

雑誌

Cell Stem Cell (ISSN:19345909)

巻号頁・発行日

vol.5, no.3, pp.237-241, 2009-08

被引用文献数

4 616

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低酸素濃度培養によるiPS細胞樹立効率の改善 -効率的な樹立方法開発に貢献する知見をCell Stem Cellに報告-. 京都大学プレスリリース. 2009-08-28. http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/090828_2.htm

著者

Yoshida Yoshinori Yamanaka Shinya

出版者

Elsevier

雑誌

Journal of Molecular and Cellular Cardiology (ISSN:00222828)

巻号頁・発行日

vol.50, no.2, pp.327-332, 2011-02

被引用文献数

123

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For the treatment of heart failure, a new strategy to improve cardiac function and inhibit cardiac remodeling needs to be established. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are pluripotent cells that can differentiate into cell types from all three germ layers both in vitro and in vivo. The therapeutic effect of ES/iPS cell-derived progeny was reported in animal model. Mouse and human somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by the transduction of four transcription factors, Oct 3/4, Sox2, Klf4, and c-Myc. However, the low induction efficiency hinders the clinical application of iPS technology, and efforts have been made to improve the reprogramming efficiency. There are variations in the characteristics in ES/iPS cell lines, and the further understanding is necessary for the applications of ES/iPS cell technology. Some improvements were also made in the methods to induce cardiomyocytes from ES/iPS cells efficiently. This review article is focused on generation of iPS cells, cardiomyocyte differentiation from ES/iPS cells, and transplantation of derived cardiomyocytes.This article is part of a special issue entitled, "Cardiovascular Stem Cells Revisited".