著者

亀山 元帥

出版者

日本医科大学医学会

雑誌

日本医科大学雑誌 (ISSN:00480444)

巻号頁・発行日

vol.63, no.2, pp.127-139, 1996-04-15 (Released:2010-03-01)

参考文献数

45

被引用文献数

1 1

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Locus control region (LCR) is known to occur 5′-upstream of the globin gene clusters in humans and a number of other animals. It comprises four DNase I hypersensitive sites, HS 1-4, and has been considered to play a key role in regulating the globin gene expression in tissue- and developmental stage-specific manners. The occurrence of LCR in the rat genome, however, has not been documented so far. In the present study, the author intended to identify and analyze the rat β-LCR HS 1 and HS 2, in order to further facilitate studies on the regulatory mechanism involved in globin gene expression. The results obtained in this study are summerized as follows:1. A DNA region of about 700 bp on the rat genome was amplified by polymerase chain reaction (PCR) using synthetic primers derived from portions of the mouse β-LCR HS 2. The nucleotide sequence of the PCR product (R 700) shows 67% and 83% homologies with those of the human and mouse HS 2, respectively, indicating that R 700 represents β-LCR HS 2 of rats.2. In order to locate β-LCR HS 2 on the rat genome, a 7 kb DNA fragment (R 7, 000) harboring a region between β-LCR HS 2 and the ε 1-globin gene was obtained by PCR. Restriction endonuclease mapping of R 7, 000 revealed that the rat β-LCR HS 2 is located 6.0kb 5′-upstream relative to the cap site of the ε 1-globin gene.3. The rat β-LCR HS 1 was then located 4.2 kb 5′-upstream of the ε 1-globin gene by Southern blot hybridization of R 7, 000 using a human HS 1 probe. Nucleotide sequencing revealed that the rat HS 1 has 83% homology to the mouse HS 1.4. Comparisons of the structures of the rat β-LCR HS 1 and HS 2 with those of other animal species indicate that several motifs and consensus sequences for binding of transcription factors, such as NF-E 2/AP-1 and GATA-1, are well conserved during evolutional periods, indicating an indispensable role of LCR in globin gene expression

著者

亀山 元帥 山中 博之 田中 隆 洪 芳樹 子島 潤 杉木 雄治 鈴木 健 大津 文雄 長沢 紘一 早川 弘一

出版者

社団法人日本循環器学会

雑誌

Japanese circulation journal (ISSN:00471828)

巻号頁・発行日

vol.57, pp.864-865, 1993-08-20