著者
西村 千昭 北岡 正見
出版者
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
雑誌
Japanese Journal of Medical Science and Biology (ISSN:00215112)
巻号頁・発行日
vol.17, no.6, pp.295-305, 1964 (Released:2010-03-19)
参考文献数
12
被引用文献数
3 6

Effort has been made to purify Japanese encephalitis (JE) virus from suckling mouse brains infected with the Nakayama strain. The brain contains so many extractable contaminating materials that purification of the virus from brain suspension by mere repetition of fractional centrifugation has been difficult (Kitaoka, 1955) . Recently, Nojima et al. (1964) reported a purification procedure of JE virus from infected suckling mouse brains by adsorption technique on hydroxyapatite column. The recent progress of sedimentation techniques has opend a simple and efficient way for purification of many animal viruses from infected tissues and tissue culture cells. The present paper will describe in detail the purification procedure of JE virus from infected suckling mouse brains by the protamine treatment, fractional centrifugation and sucrose gradient centrifugation. Also the structure of virus particles will be discussed in regard to hemagglutinating (HA) and complement fixing (CF) substances and infectious principle.
著者
浅沼 靖 宮本 武美 秋山 順 中川 宏 堀 覚 北岡 正見 大久保 薫 熊田 信夫 鈴木 誠 柄沢 敏夫 久郷 準 山本 久 川村 明義
出版者
日本衛生動物学会
雑誌
衛生動物 (ISSN:04247086)
巻号頁・発行日
vol.10, no.4, pp.232-244, 1959
被引用文献数
1 4

Among the cases of scrub typhus newly recognized in Japan after the World War II, those prevalent in the Izu Shichito Islands of Tokyo Prefecture, in Awa-gun, Chiba Prefecture, and in Mt. Fuji area, Shizuoka Prefecture, are called as the "Shichito type scrub typhus" or as the "scutellaris type scrub typhus, " and Trombicula scutellaris has been presumably incriminated as a potential vector of this type of scrub typhus. The study on scrub typhus in Chiba Prefecture was commenced in 1952, when the so called "twentydays fever, " an endemic disease in this Prefecture, was assumed to belong to a scrub typhus by Kasahara et al. (1952) and Kitaoka et al. (1952).