著者

王 青躍 栗原 幸大 桐生 浩希 坂本 和彦 三輪 誠 内山 巌雄

出版者

日本エアロゾル学会

雑誌

エアロゾル研究 = Journal of aerosol research (ISSN:09122834)

巻号頁・発行日

vol.23, no.2, pp.120-126, 2008-06-20

被引用文献数

3

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In this study, we focus on the investigation of the airborne behavior of respirable particulate matters containing Japanese cedar pollen allergen Cry j 1 exfoliated from the Ubisch bodies on the pollen surfaces and Cry j 2 comprised in the starch granules within the pollen. The airborne Japanese cedar pollens were counted, and at the same time, the Cry j 1 and Cry j 2 particles were collected and determined in different particle sizes at the urban site (Cooperative Research Center of Saitama University) during daily sampling periods of the most severe pollination season of FY 2005 in Saitama, Japan.<BR>Morphological observation of Ubisch bodies exfoliated from pollen and ruptured pollens was performed with a scanning electron microscope. Moreover, the existing evidences of visualized Cry j 1 and Cry j 2 allergen particles below 1.1&mu;m were also sensitively found by an immunofluorescence technique with a fluorescence microscope. Simultaneously, the high concentrations of Cry j 1 and/or Cry j 2 were determined in the fine particle sizes below 1.1&mu;m. It was suggested that airborne respirable particulate matters containing Cry j 1 and Cry j 2 play a key role in the increasing incidence of asthma in the lower respiratory tract. Furthermore, although the release mechanisms of Cry j 1 and a Cry j 2 particles may be differed, the daily variation delays in the peaks of Cry j 1 (or Cry j 2) concentrations compared to the peaks of airborne pollen counts were observed since the fine particles were suspended in the atmosphere over a longer period of time after released. It is important to observe and determine the size distribution of airborne Japanese cedar pollen allergen particles in the long-term sampling research continually, and to elucidate the allergen particle release mechanisms. In the future, we should investigate the chemical, physical and biomedical combination effects on Japanese cedar pollen allergens, and have to consider proposing a new forecast informational system of the Japanese cedar pollen and their allergen particle variations.

著者

王 青躍 栗原 幸大 桐生 浩希 坂本 和彦 三輪 誠 内山 巌雄

出版者

日本エアロゾル学会

雑誌

エアロゾル研究 (ISSN:09122834)

巻号頁・発行日

vol.23, no.2, pp.120-126, 2008-06-20 (Released:2008-06-25)

参考文献数

22

被引用文献数

2

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In this study, we focus on the investigation of the airborne behavior of respirable particulate matters containing Japanese cedar pollen allergen Cry j 1 exfoliated from the Ubisch bodies on the pollen surfaces and Cry j 2 comprised in the starch granules within the pollen. The airborne Japanese cedar pollens were counted, and at the same time, the Cry j 1 and Cry j 2 particles were collected and determined in different particle sizes at the urban site (Cooperative Research Center of Saitama University) during daily sampling periods of the most severe pollination season of FY 2005 in Saitama, Japan.Morphological observation of Ubisch bodies exfoliated from pollen and ruptured pollens was performed with a scanning electron microscope. Moreover, the existing evidences of visualized Cry j 1 and Cry j 2 allergen particles below 1.1μm were also sensitively found by an immunofluorescence technique with a fluorescence microscope. Simultaneously, the high concentrations of Cry j 1 and/or Cry j 2 were determined in the fine particle sizes below 1.1μm. It was suggested that airborne respirable particulate matters containing Cry j 1 and Cry j 2 play a key role in the increasing incidence of asthma in the lower respiratory tract. Furthermore, although the release mechanisms of Cry j 1 and a Cry j 2 particles may be differed, the daily variation delays in the peaks of Cry j 1 (or Cry j 2) concentrations compared to the peaks of airborne pollen counts were observed since the fine particles were suspended in the atmosphere over a longer period of time after released. It is important to observe and determine the size distribution of airborne Japanese cedar pollen allergen particles in the long-term sampling research continually, and to elucidate the allergen particle release mechanisms. In the future, we should investigate the chemical, physical and biomedical combination effects on Japanese cedar pollen allergens, and have to consider proposing a new forecast informational system of the Japanese cedar pollen and their allergen particle variations.

著者

橋本 博 菱木 麻美 原 幸大 菊池 壮太郎

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.57, no.1, pp.015-019, 2017 (Released:2017-01-26)

参考文献数

20

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DNA damage tolerance (DTT) is a cell function to avoid replication arrest by DNA damage during DNA replication. DTT includes two pathways, translesion DNA synthesis (TLS) and template-switched DNA synthesis (TS), regulated by various molecular interactions. TLS is transient DNA synthesis using a damaged template by error-prone DNA polymerases specialized for DNA damage (TLS polymerases). TS, in which one newly synthesized strand is utilized as an undamaged template for replication by replicative polymerases, is error-free process. This review article describes recent progress in structural studies of proteins involved in TLS and TS.