著者
吉村 研治 北島 力 宮本 義次 岸本 源次
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.60, no.2, pp.207-213, 1994-03-25 (Released:2008-02-29)
参考文献数
9
被引用文献数
12 14

In order to investigate the factors inhibiting the growth of rotifer (S-type) in high density cultivation, a series of experiments were conducted, cultivating rotifer in 30l and 1kl vessels by feeding condensed Chlorella. Although there was no difference in the maximum population of rotifer in the culture experiments at different temperatures of 24, 28, and 32°C, the growth rate increased as the culture temperature increased. In the cultivation system supplied with air, the maximum rotifer population did not exceed 4, 000-5, 000 individuals/ml because oxygen levels were restricted due to the accumulation of undissociated ammonia. Feeding oxygen gas to the rotifer culture system enabled high density cultivation with a population of 20, 000 individuals/ml. In such an oxygen-rich culture system, the concentration of undissociated ammonia is considered to be the main factor inhibiting the growth of rotifer.
著者
吉村 研治 大森 庸子 吉松 隆夫 田中 賢二 石崎 文彬
出版者
日本水産學會
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.62, no.6, pp.897-903, 1996-11-15
参考文献数
6
被引用文献数
7 7

In a high density culture of rotifer (beyond the levels of 10<sup>3</sup>ind./m<i>l</i>), the amount of aeration needed to maintain an optimum dissolved oxygen (DO) level increases. In order to investigate the effect of aeration on the population growth of rotifer, a series of experiments were carried out. The S-type rotifer <i>Brachionus rotundiformis</i> was cultured at high density using 10<i>l</i> vessels under different aeration conditions. Air or oxygen gas was introduced into the culture media through two types of sparger which formed different sizes of bubbles, at aeration rates from 0.1 to 2.0vvm. The bubble-size from the two kinds of sparger were estimated from the value of <i>KLa</i> (volumetric oxygen transfer coefficient). When the aeration rate was high or the ceramic sparger that formed smaller bubbles was used, DO in the culture system remained at a high level. However, under these conditions, the growth of the rotifer population was seriously inhibited due to very high aeration rates and small bubbles, possibly as a result of foaming separation. When oxygen gas was introduced into the culture system, the density of rotifer was higher than that when air was introduced. Therefore, it is recommended to introduce high-purity oxygen gas for a high density culture to avoid any growth inhibition and to maintain optimum DO.