著者
本多 一郎 和田 道宏 阿部 知子 浅見 忠男 吉田 茂男
出版者
植物化学調節学会
雑誌
植物化学調節学会研究発表記録集 (ISSN:09191887)
巻号頁・発行日
no.35, pp.147-148, 2000-11-02

To obtain novel mutants of barley (Horderum vulgare L), heavy ion irradiation were conducted. Both imbibed and dry seeds of barley (Kanto Hadaka No.77) were irradiated with 5-400 [gray] of dose by heavy-ion beam (^<14>N; 135MeV/u) and cultivated on field. Severe growth inhibition were observed in over 10 and 200 gray irradiation in imbibed and dry seed irradiation, respectively. After harvesting M_2 seeds, an aliquot of seeds were sown to field, candidates of mutants were serveyed, and selected. After crossing, their F_1 and F_2 plant phenotype were examined, 3 dwarf lines which have ressesive allele were separated. These results suggest that heavy-ion irradiation is effective method to mutate barley.
著者
金澤 裕太 野地 なつ美 齊藤 隆夫 吉田 茂男 浅見 忠男
出版者
一般社団法人 植物化学調節学会
雑誌
植物化学調節学会 研究発表記録集 (ISSN:09191887)
巻号頁・発行日
vol.39, pp.90, 2004-10-13 (Released:2018-02-15)

Pyrroloquinoline quinone(PQQ; 1) was discovered as a novel co-factor in various bacterial alcohol dehydrogenases in 1979. In 2003, it was reported that PQQ is nutritionally important as a vitamin in mammals. Various analytical methods have been reported for the quantification of free PQQ, but their reports have not established a detailed procedure for the assay of PQQ in crude biological samples. In this study, we have synthesized ^<13>C-labeled PQQ to use this chemical as an internal standard. We synthesized the labeled PQQ from 5-amino-2-methoxyformanilide(2) by applying Corey's method in a key step.