著者

小田 隆治 鹿取 信

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.11, no.1, pp.9-17, 1991-01-10 (Released:2010-04-12)

参考文献数

30

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A series of the process of neutrophil extravasation induced by topical application of leukotriene B4 (LTB4) or formyl-methionyl-leucyl-phenylalanine (fMLP) on microvasculature of the hamster cheek pouch can be divided into five steps; I) rolling on the venular endothelium, II) adhesion on the endothelium, III) passage between the endothelial cells, IV) staying in the venular wall, V) migration from the venular wall to the interstitial space. In step II, topical application of chemoattractants on the microvasculature caused an increase in the number of neutrophils adhered to the venules. In step III, the size of the adhered neutrophils became gradually smaller and finallly disappear from the vascular lumen, as they were observed on the monitor screen. Over 80-90% of the adhered neutrophils passed through the endothelial cells. The whole process from step II to III took about 7 minutes. In step IV, the neutrophils which passed through the endothelial cells stay for about 30 minutes in the venular wall. In step V, neutrophils penetrated the basement membrane and migrated into the interstitial space.Step II and III were suppressed by a cyclic AMP phosphodiesterase inhibitor, fibronectin inhibitor and cytochalasin B. From these results, it was suggested that chemoattractant may cause the contraction of microfilament of neutrophil, and the contraction may induce the expression of adhesive glycoprotein on the neutrophil membrane. The increase of cyclic AMP in the neutrophil may inhibit the contraction of microfilament and/or the expression of adhesive glycoprotein of neutrophil. Dexamethasone did not affect the step I to IV, but suppressed the step V. Dexamethasone may inhibit synthesis and/or release of protease (s) in the neutrophil granules which degrade the basement membrane. In fact, the collagenase inhibitor suppressed the step V, although it did not the step I to IV. Thus, neutophil-derived collagenase may act on the passage of neutrophil through the basement membrane. The process of neutrophil extravasation is the chain of reaction.

著者

渡邊 真弓 佐藤 友美子 小田 隆治

出版者

東北・北海道地区大学等高等・共通教育研究会

雑誌

東北・北海道地区大学等高等・共通教育研究会研究集録

巻号頁・発行日

vol.61, pp.91-95, 2011-09-01

著者

杉原 真晃 橋爪 孝夫 時任 隼平 小田 隆治

出版者

日本教育工学会

雑誌

日本教育工学会論文誌 (ISSN:13498290)

巻号頁・発行日

vol.38, no.4, pp.341-349, 2015-03-20 (Released:2016-08-11)

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本研究では,大学初年次教養教育科目としてのサービス・ラーニングにおける現地での活動の質の向上という課題に対して,地域住民とともに活動を行う参与型の実践,評価者に地域住民も加わる実践,地域住民と教員が協働で評価基準を開発・活用するケースについて,評価基準の協働作成と学生への提示の有効性を検討した.その結果,現地での活動の目標の意識化・再設定,意義・方法・役割の明確化,現地の人々や他学生との意義の共有・一体感の獲得等に対して,評価基準が有用であることが明らかとなった.学習成果との関連については,評価基準に示された項目の中で,現地にて重要視されており,学生がそれを達成し,評価基準がそれに対して有用であったと感じられた項目が多い結果となり,本授業で活動・学習の質の保証・向上が概ね達成されており,協働作成した評価基準が有効に機能したことが明らかとなった.