- 一般社団法人 日本輸血・細胞治療学会
- 日本輸血学会雑誌 (ISSN:05461448)
- vol.36, no.5, pp.580-586, 1990-10-30 (Released:2010-03-12)
Irradiation with X-ray to blood products is a standard practice recommended for prevention of post-transfusion graft versus host disease in patients. In order to seek the optimal condition of irradiation to minimize the lesion of red blood cell (RBC) with complete inactivation of lymphocytes, we studied the effects of irradiation with X-ray on the quality of RBC in whole blood (WB) and concentrated red blood cells (CRC) in the range of 5 to 50 Gy.X-ray irradiation did not alter ATP and 2, 3-DPG content, hemolysis ending point and morphology of RBC, while it caused a slight increase of osmotic pressure at hemolysis starting point of RBC and potassium concentration in plasma. Considerable increase of hemolysis was observed with higher dose of irradiation in both WB and CRC. However, it was so small as below 2-3×10-2 percent that is acceptable level in blood products.Therefore, we concluded that the X-ray irradiation of WB and CRC with up to 50 Gy has no significant effects on in vitro characteristics of RBC.