著者
中島 尚登 星 順隆 浅井 治 山本 純子 竹内 直子 神谷 昌弓 加藤 敦子 長谷川 智子 山崎 恵美 中山 一
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.37, no.6, pp.795-802, 1991-12-25 (Released:2010-03-12)
参考文献数
18

A new red cell preservation solution is described in which the red cells may be stored for 42 and 49 days. An in vivo and vitro study of 42 and 49 days stored red cells with saline, adenine, glucose, mannitol (SAGM) preservation solution (OPTISOL, Terumo Corporation) was conducted using 20 healthy volunteers to document in vivo efficacy and analyze the validity of the 51Cr technique. Standard in vitro parameters of OPTISOL red cell concentrates were well preserved with low levels of hemolysis and high levels of red cell ATP which is compatible with their survival after 42 and 49 days storage. Osmotic pressure at hemolysis ending point of 42 and 49 days stored red cells did not change, while osmotic pressure at hemolysis starting point increased on 49 days. The red cell viscosity increased and scanning electron-microscopic studies showed that the majority of them became echinocyte and spherocyte on 49 days. In vivo autologous post-transfusion recovery was measured by using a 51Cr-labeled red cells. After 42 days of storage, post-transfusion 24-hour recovery averaged 82.3±8.4% and 48-hour recovery was 79.1±9.1%, and after 49 days, 24-hour recovery was 75.9±3.6%, but 48-hour recovery, which averaged 69.9±5.2%, was significantly lower.The present study reports in vivo and in vitro evaluation of a new red cell storage medium which allows high levels of post-transfusion recovery and permits without significant hemolysis. The results suggested that 42 days if a more suitable shelf life than 49 days.
著者
福森 泰雄 田中 成憲 杉本 昭子 大軒 子郎 山口 英夫
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.36, no.5, pp.580-586, 1990-10-30 (Released:2010-03-12)
参考文献数
13

Irradiation with X-ray to blood products is a standard practice recommended for prevention of post-transfusion graft versus host disease in patients. In order to seek the optimal condition of irradiation to minimize the lesion of red blood cell (RBC) with complete inactivation of lymphocytes, we studied the effects of irradiation with X-ray on the quality of RBC in whole blood (WB) and concentrated red blood cells (CRC) in the range of 5 to 50 Gy.X-ray irradiation did not alter ATP and 2, 3-DPG content, hemolysis ending point and morphology of RBC, while it caused a slight increase of osmotic pressure at hemolysis starting point of RBC and potassium concentration in plasma. Considerable increase of hemolysis was observed with higher dose of irradiation in both WB and CRC. However, it was so small as below 2-3×10-2 percent that is acceptable level in blood products.Therefore, we concluded that the X-ray irradiation of WB and CRC with up to 50 Gy has no significant effects on in vitro characteristics of RBC.
著者
天野 尹
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.29, no.6, pp.597-606, 1984 (Released:2010-03-12)
参考文献数
8

The term of 37 years, from 1925 to 1961, could be divided into five periods. The first corresponds to one with a stable and high birth rate, the second to that of Japan-China War, the third to that of World War II, the fourth to that of postwar with its rapid drop in birth rate and the fifth to that with a low birth rate since then. This classification of period shows the change of historical conditions of Japan.The materials for the test of homogeneity were prepared from random sample from the cards of the registered donors in Shimane, Yamaguchi, Akita and Iwate Prefectures and Bernstein's method was applied to estimate the gene frequencies (p: gene of A, q: gene of B, r: gene of O).The results of the test of homogeneity between each period could be expressed as follows: in Shimane Prefecture; q in the third period decreased, in Akita Prefecture; q in the third peroid decreased, in Yamaguchi Prefecture; p in the fourth period increased and in Iwate Prefecture, q in the latter half of the fifth period decreased.The effect of hybridisation, areal biased donation, immigration and inbreeding must be considered as possible factors in the change of gene frequency.There is very little hybridisation in Japan and is not relevant here.Areal biased donation which means an increase or a decrease of coming donors from particular districts was demonstrated in the survey of Shimane blood center, and in the population size of the blood center level this biased donation shows no significant change of gene frequency.An assumption of 4% immigration, from Akita to Shimane Prefecture, revealed no change of gene frequency, but when immigration amounted to 10% in two or more years the test verified that there is a significant change in the distribution of gene frequency. But such an excessive immigration is not the real condition in prefectures of country-side.If there is an effect of inbreeding, there must be a significant difference of p2, q2 and r2 between the first period and the fifth period, since the fifth period corresponds to the second generation of the first period. But the results of these computations were not significant in any of the four prefectures. The change of previously mentioned social and historical conditions have led to decrease of the number of birth, the fractionising of population, the temporary isolation of inhabitans and then subsequent relaease of fractionising and isolation. The consequence of these changes is the so called “bottle-neck effect”.In countries of the world where a high frequency of hybridisation takes place the results such as mentioned here would be difficult to obtain.
著者
菊地 金男 正宗 良知
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.33, no.6, pp.710-715, 1987 (Released:2010-03-12)
参考文献数
9

Relation between perioperative blood transfusion and prognosis after surgery for gastric cancer was studied retrospectively on 2086 patients who had undergone histologically curative operation in the 2nd study of Cooperative Study Group of Surgical Adjuvant Chemotherapy for Gastric Cancer. These patients were divided into the 3 groups according to the administraiton of anticancer drugs (A: MMC, B: MMC+FT, C: FT). The survival and recurrence rate of transfused patients were compared with those of non-transfused patients in each histological stage of A, B and C groups.Macroscopic factors of recurrence such as lymph nodal metastases, tumor size and serosal invasion in transfused patients were overestimated, and total gastrectomy with splenectomy and wide extirpation of lymph nodes were performed along with blood transfusion. But microscopic factors of recurrence in transfused patients were better than in non-transfused patients.Five-year actuarial survival rate in transfused patients was generally lower than in non-transfused patients. Especialy the 5-year survival rates of transfused patients in stage II of A, C groups and stage III of A, B groups were respectively lower than those of non-transfused patients. Five-year actuarial recurrence rate in transfused patients was higher than in non-transfused patients. The 5-year recurrence rates of transfused patients in stage I of A, B groups, stage II of A group and stage III of B group were severally higher than those of non-transfused patients. Those rates were statistically significant.
著者
内田 立身 西村 拓史 瀧本 宏美 佐藤 美津子 西尾 由美子 福家 洋子 野崎 正範
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.42, no.5, pp.215-217, 1996-10-01 (Released:2010-03-12)
参考文献数
8
被引用文献数
1

We evaluated the effect of repeated 400ml whole blood donation on storage iron status in 26 males and 196 females. Iron status was estimated from hemoglobin concentration, transferrin saturation and serum ferritin. Storage iron was calculated according to the formula proposed by the National Health and Nutrition Examination Survey (NHANES). Calculated storage iron after repeated donation of 400ml was 806±174mg in male donors and 292±248mg in females. Levels after 400ml donation decreased to 606mg in males and to 92mg in females. Among females, 20.5% were in negative iron balance before donation, increasing to 37.7% after donation. However, no significant decrease in storage iron by repeated blood donation was recognized, when standards for blood collection were strictly complied with.
著者
松本 美富士 由良 二郎
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.37, no.6, pp.782-786, 1991-12-25 (Released:2010-03-12)
参考文献数
13

Sera from patients with collagen diseases followed at Nagoya City University Hospital were investigated for the detection of antibody (C100-3) to hepatitis C virus (HCV), and HCV-RNA using the method of reverse transcriptase polymerase chain reaction (RT-PCR). The overall prevalance of HCV antibody positivity was 4.8% (12/252) in the patients, and significantly higher than that (1.1-1.2%) of Japanese populations of blood donors. Relative high prevalence (6.5%) was observed in patients with Sjogren's syndrome (SjS). Seven of 12 patients with anti-HCV antibodies were detected HCV-RNA in their sera. All of these patients, except one case, had chances of the infection for HCV. 5 patients, whose sera was negative result of HCV-RNA, had no history of HCV infection. Therefore, the presence of false-positive tests for anti-HCV antibodies was 2.0% (5/245) in patients with collagen diseases. Sera from patients with SjS showed the higher false-positive tests. The false-positive tests for antibodies to HCV did not correlate with the presence of antinuclear antibodies, rheumatoid factor, and hypergamma-globulinemia.
著者
本庶 佑
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.45, no.4, pp.476-478, 1999-08-01
参考文献数
5
著者
中野 節子 今井 美智子 小金丸 智子 原 邦子 上田 信子 堤 康英 大山 正則 入田 美子 清川 博之 前田 義章
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.41, no.6, pp.612-616, 1995 (Released:2010-03-12)
参考文献数
8

Three apheresis systems (COBE Sprectra, Fenwal CS-3000 plus and Haemonetics MCS) were compared with regard to white cell (WBC) content (n=9 each) in the apheresis chamber on plateletpheresis of 10 units (range, 2-3×1011). Average WBC content in the residual blood in Sprectra, CS-3000 plus and MCS was 6.65×108 (70% of lymphocytes), 3.98×108 (77% of lymphocytes) and 2.21×108 (41% of lymphocytes) WBCs, respectively.Apheresis donors experiencing 12 plateletpheresis procedures per year, the maximum allowed, will sustain a 56.4×108 lymphocyte loss when Spectra, designoed to collect WBC-poor platelet concentrates, is used.The annual loss of lymphocytes in males and females would be 2.5 and 3.7 times, that by whole blood donation. Recently developed respectively, cell separators have been improved to decrease WBC contamination. However, close attention should be paid to WBC content in residual blood in the apheresis chamber.
著者
林 正則
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.41, no.3, pp.300-303, 1995 (Released:2010-03-12)
参考文献数
15
被引用文献数
1
著者
井上 千加子 神谷 忠 小澤 和郎
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.47, no.1, pp.22-28, 2001-02-01 (Released:2010-03-12)
参考文献数
12

In 1999, the number of donors with HIV antibodies was 63, giving a prevalence of 1.026per 100, 000 donations collected by the Japanese Red Cross Society Blood Centers. The current frequency is higher than would be expected from the prevalence rate of HIV in Japan. We conducted a questionnaire to determine blood donor understanding of HIV. The survey looked at responses anonymously from 676 apheresis donors who donated at 8 collection offices in Aichi Prefecture in mid-January, 2000. It indicated that almost all of them were really comprehensive and 27% of respondents supported the present policy of not letting donors know the results of their HIV tests. But a tiny fraction of repeated donors did not understand why they should not donate blood as a means of checking on HIV.To reduce the risk of transfusion-transmitted infection, nucleic acid amplification test screening for HIV, HBV and HCV was introduced for all donated blood in October 1999. However, based on the rapid spread of sexually transmitted diseases and drug abuse especially among younger people, it is essential for ensuring blood safety that adequate donor education be conducted along with various approaches to reduce blood-borne infection including HIV.
著者
牧田 光雄 関根 活郎 山田 雅人
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.5, no.4, pp.145-146, 1958 (Released:2010-03-12)
参考文献数
3

The classification of the mouse blood types was described and the following results were obtained.1. Applying human anti-A and anti-B sera as agglutinins, the blood cells of the mouse were divided into four groups:a) agglutinated by human anti-A sera (17.4per cent)b) agglutinated by human anti-B sera (13.9per cent)c) agglutinated by human anti-A and anti-B sera (48.2per cent)d) neither agglutinated by human anti-A nor anti-B sera (20.5per cent)2. The blood cells of some mice were agglutinated by human AB blood type sera (69.6per cent). This result indicated that human AB blood type sera included certain agglutinins against mouse blood cells.3. Based on the antigenic analysis of blood types of the mouse by means of anti M I and M II sera of immunized rabbits, the blood cells of mouse were divided into four groups:a) M I type (13.3per cent)b) M II type (40.9per cent)c) M I, M II type (43.8per cent)d) X (non-agglutinated) type (2.0per cent)