著者
天野 尹
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.29, no.6, pp.597-606, 1984 (Released:2010-03-12)
参考文献数
8

The term of 37 years, from 1925 to 1961, could be divided into five periods. The first corresponds to one with a stable and high birth rate, the second to that of Japan-China War, the third to that of World War II, the fourth to that of postwar with its rapid drop in birth rate and the fifth to that with a low birth rate since then. This classification of period shows the change of historical conditions of Japan.The materials for the test of homogeneity were prepared from random sample from the cards of the registered donors in Shimane, Yamaguchi, Akita and Iwate Prefectures and Bernstein's method was applied to estimate the gene frequencies (p: gene of A, q: gene of B, r: gene of O).The results of the test of homogeneity between each period could be expressed as follows: in Shimane Prefecture; q in the third period decreased, in Akita Prefecture; q in the third peroid decreased, in Yamaguchi Prefecture; p in the fourth period increased and in Iwate Prefecture, q in the latter half of the fifth period decreased.The effect of hybridisation, areal biased donation, immigration and inbreeding must be considered as possible factors in the change of gene frequency.There is very little hybridisation in Japan and is not relevant here.Areal biased donation which means an increase or a decrease of coming donors from particular districts was demonstrated in the survey of Shimane blood center, and in the population size of the blood center level this biased donation shows no significant change of gene frequency.An assumption of 4% immigration, from Akita to Shimane Prefecture, revealed no change of gene frequency, but when immigration amounted to 10% in two or more years the test verified that there is a significant change in the distribution of gene frequency. But such an excessive immigration is not the real condition in prefectures of country-side.If there is an effect of inbreeding, there must be a significant difference of p2, q2 and r2 between the first period and the fifth period, since the fifth period corresponds to the second generation of the first period. But the results of these computations were not significant in any of the four prefectures. The change of previously mentioned social and historical conditions have led to decrease of the number of birth, the fractionising of population, the temporary isolation of inhabitans and then subsequent relaease of fractionising and isolation. The consequence of these changes is the so called “bottle-neck effect”.In countries of the world where a high frequency of hybridisation takes place the results such as mentioned here would be difficult to obtain.
著者
菊地 金男 正宗 良知
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.33, no.6, pp.710-715, 1987 (Released:2010-03-12)
参考文献数
9

Relation between perioperative blood transfusion and prognosis after surgery for gastric cancer was studied retrospectively on 2086 patients who had undergone histologically curative operation in the 2nd study of Cooperative Study Group of Surgical Adjuvant Chemotherapy for Gastric Cancer. These patients were divided into the 3 groups according to the administraiton of anticancer drugs (A: MMC, B: MMC+FT, C: FT). The survival and recurrence rate of transfused patients were compared with those of non-transfused patients in each histological stage of A, B and C groups.Macroscopic factors of recurrence such as lymph nodal metastases, tumor size and serosal invasion in transfused patients were overestimated, and total gastrectomy with splenectomy and wide extirpation of lymph nodes were performed along with blood transfusion. But microscopic factors of recurrence in transfused patients were better than in non-transfused patients.Five-year actuarial survival rate in transfused patients was generally lower than in non-transfused patients. Especialy the 5-year survival rates of transfused patients in stage II of A, C groups and stage III of A, B groups were respectively lower than those of non-transfused patients. Five-year actuarial recurrence rate in transfused patients was higher than in non-transfused patients. The 5-year recurrence rates of transfused patients in stage I of A, B groups, stage II of A group and stage III of B group were severally higher than those of non-transfused patients. Those rates were statistically significant.
著者
内田 立身 西村 拓史 瀧本 宏美 佐藤 美津子 西尾 由美子 福家 洋子 野崎 正範
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.42, no.5, pp.215-217, 1996-10-01 (Released:2010-03-12)
参考文献数
8
被引用文献数
1

We evaluated the effect of repeated 400ml whole blood donation on storage iron status in 26 males and 196 females. Iron status was estimated from hemoglobin concentration, transferrin saturation and serum ferritin. Storage iron was calculated according to the formula proposed by the National Health and Nutrition Examination Survey (NHANES). Calculated storage iron after repeated donation of 400ml was 806±174mg in male donors and 292±248mg in females. Levels after 400ml donation decreased to 606mg in males and to 92mg in females. Among females, 20.5% were in negative iron balance before donation, increasing to 37.7% after donation. However, no significant decrease in storage iron by repeated blood donation was recognized, when standards for blood collection were strictly complied with.
著者
井上 千加子 神谷 忠 小澤 和郎
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.47, no.1, pp.22-28, 2001-02-01 (Released:2010-03-12)
参考文献数
12

In 1999, the number of donors with HIV antibodies was 63, giving a prevalence of 1.026per 100, 000 donations collected by the Japanese Red Cross Society Blood Centers. The current frequency is higher than would be expected from the prevalence rate of HIV in Japan. We conducted a questionnaire to determine blood donor understanding of HIV. The survey looked at responses anonymously from 676 apheresis donors who donated at 8 collection offices in Aichi Prefecture in mid-January, 2000. It indicated that almost all of them were really comprehensive and 27% of respondents supported the present policy of not letting donors know the results of their HIV tests. But a tiny fraction of repeated donors did not understand why they should not donate blood as a means of checking on HIV.To reduce the risk of transfusion-transmitted infection, nucleic acid amplification test screening for HIV, HBV and HCV was introduced for all donated blood in October 1999. However, based on the rapid spread of sexually transmitted diseases and drug abuse especially among younger people, it is essential for ensuring blood safety that adequate donor education be conducted along with various approaches to reduce blood-borne infection including HIV.
著者
牧田 光雄 関根 活郎 山田 雅人
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.5, no.4, pp.145-146, 1958 (Released:2010-03-12)
参考文献数
3

The classification of the mouse blood types was described and the following results were obtained.1. Applying human anti-A and anti-B sera as agglutinins, the blood cells of the mouse were divided into four groups:a) agglutinated by human anti-A sera (17.4per cent)b) agglutinated by human anti-B sera (13.9per cent)c) agglutinated by human anti-A and anti-B sera (48.2per cent)d) neither agglutinated by human anti-A nor anti-B sera (20.5per cent)2. The blood cells of some mice were agglutinated by human AB blood type sera (69.6per cent). This result indicated that human AB blood type sera included certain agglutinins against mouse blood cells.3. Based on the antigenic analysis of blood types of the mouse by means of anti M I and M II sera of immunized rabbits, the blood cells of mouse were divided into four groups:a) M I type (13.3per cent)b) M II type (40.9per cent)c) M I, M II type (43.8per cent)d) X (non-agglutinated) type (2.0per cent)
著者
稲葉 頌一 大戸 斉 柴田 洋一 坂本 久浩 高橋 孝喜 十字 猛夫 前田 義章
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.40, no.1, pp.1-13, 1994-01-25 (Released:2010-03-12)
参考文献数
9

Sixty-six patients who succeeded in storing a sufficient volume of autologous blood (mean±SD; 1097±452ml) using KL-3R3 solution contained in blood bags before elective surgery were included in this study, a five week phase 3 clinical trial of the whole blood preservative solution KL-3R3, commonly known as CPDA-1 (citrate phosphate dextrose adenine) solution. The mean time required for sufficient storage of autologous blood was 23.4±6.9 days, with 40 of the 66 (60.6%) requiring more than 21 days, a period of time that would have resulted in time expiration had we used the citrate-phosphate-dextrose (CPD) or acid-citrate-dextrose (ACD) solution bags which are presently commercially available. Sixty of the 66 patients (90.9%) completed their operations using only autologous blood units. After eliminating three patients who were administered recombinant erythropoietin, the efficacy of KL-3R3 was evaluated as effective in 61 (96.8%) of the 63 patients evaluated (markedly effective 57 (86.4%) and effective 4 (6.1%). Furthermore, in 48 of 56 patients (85.7%), in whom we were able to measure the autologous blood recovery rate after 24 hours of transfusion were estimated effective (markedly effective 29 (51.8%) and effective 19 (33.9%)). In sixty-three patients who had hematological and biochemical laboratory data, vital signs, and urinary data recorded both before and after receiving autologous blood units. RBC counts and hemoglobin levels and platelets counts decreased, and WBC counts increased after returning autologous blood at operation, changes observed commonly under surgical stress. Hemolysis markers such as GPT, indirect bilirubin, and LDH were slightly increased the day after operation, but returned to normal the second day after operation. Other data moved within normal limits expect urine occult blood. Hematuria was observed immediately after transfusion of autologous blood units, but this finding was observed transiently (the first day after transfusion 45.7% and the second day 13.3%). The safety was evaluated in sixty-five patients' blood bags by sight observations and bacterial culture tests, and no abnormal findings or bacterial propagation were detected. One patient was excluded from the safety evaluation because he was operated on within a week of initial predeposition. Two-hundred-thirty-eight KL-3R3 preserved blood bags were transfused in the 66 patients, in one (1/238, 0.4%) which had be discarded because of a large clot formation. In sixty-one patients, both the efficacy and the safety tests of KL-3R3 were rated for usefulness, with all 61 confirmed useful. The laboratory data of the 26 patients whose autologous blood were used within 21 days and the 40 patients whose autologous blood contained at least one unit that exceeded 21 days preservation was compared, with no significant differences observed.Our results confirmed that, in clinical trial of KL-3R3 preserved autologous blood units, all the criteria of effectiveness, safety and usefulness were satisfied.
著者
小暮 正久
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.19, no.4-6, pp.161-166, 1973 (Released:2010-03-12)
参考文献数
26
被引用文献数
1 1

When group O red cells were incubated with UDP-Galactose and serum from group B or AB subjects, they became strongly agglutinable by human anti-B serum. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:128.Group O red cells incubated with UDP-Galactose and serum from group Bm or A1Bm subjects were converted to B active cells. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:8 or 1:16.Comparison of the agglutination titers of the converted cells against human anti-B serum shows that the activity of serum α-galactosyltransferase from group Bm subjects is approximately one-eighth or one-sixteenth of that from group B subjects.When group O red cells were incubated with UDP-Galactose and serum from group Cis AB subjects, they could not be agglutinated by human anti-B serum. The activity of serum α-galactosyltransferase from group Cis AB subjects could not be demonstrable by agglutination test. This is considered to be associated with the regularly present cold anti-B agglutinins in the sera of group Cis AB subjects.
著者
北濱 睦夫 山口 英夫 硲 文雄 大久保 康人
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.13, no.3, pp.81-91, 1966 (Released:2010-03-12)
参考文献数
37

A 38-year-old healthy Japanese woman Mrs. N. has as much B and H substances in her saliva as have normal B individuals, but the B antigen in her red cells is very weak and H is undetectable.Her serum contains weak anti-H. It was considered that Mrs. N. 's blood might be called Bh at the phenotypical level; the suffix “h” is intended to indicate a phenotype lacking the H substance.
著者
三谷 孝子 橋本 浩司 千葉 眞彰 池淵 研二 関口 定美
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.42, no.6, pp.294-298, 1996-12-01 (Released:2010-03-12)
参考文献数
17
被引用文献数
2 1

Bacterial contamination was observed in a platelet concentrate obtained by apheresis using COBE SPECTRA and stored for 45 hours with agitation at 20 to 24°C. The platelet product was contaminated with 107 CFU/ml of Bacillus cereus. The contamination was likely attributable to bacteria on the skin at the phlebotomy site, because 1) apheresis was performed in a closed system and 2) Bacillus cereus are known to exist ubiquitously including the skin surface, and to be resistant to the chlorohexidine-ethanol that is routinely used for disinfection of phlebotomy site in our blood center. In a spike experiment with apheresis platelets, the growth of Bacillus cereus was vey rapid, with a doubling time of 6 hours to reach 107 CFU/ml in 2 days.Leukocyte depletion resulted in reduced phagocytic activity and allowed rapid growth of the contaminating bacteria in platelet products. There findings enpharize the importance of preventing as far as possible. In addition, platelet recipients, largely comprising leukemia patients, are vulnerable to infection.
著者
森 和久
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.8, no.3-4, pp.310-326, 1962 (Released:2010-03-12)
参考文献数
74

This experiment was attempted to bring to light the production mechanism of auto-antibody, homologous and heterologous antibodies to red cells, as well as the destruction mechanism in vivo of red cells caused by the presence of these antibodies.Rabbits were used for the experiment.Consideration was advanced principally concerning the determination of the activity value of Cr51 labelled on red cells, availing of the specific and convenient nature of isotope Cr51 and red cells in the ability of association and dissociation with each other, and at the same time a serological experiment on the serum was jointly performed for the consideration.The survival time in vivo of the same typed as well as different typed red cells was measured after the rabbit's blood was typed with antisera, with the result that the loss curve of both the same type and the different type cells practically coincided with that of the auto-cells.The repeated infusion of enzyme (trypsin) treated auto-cells into normal and splenectomized rabbits reveals an increased rapidity in the loss of the trypsin treated auto-cells with added frequency of the repetition. On further examining the serum of this particular rabbit, the presence of an antibody to auto-cells was proved.The experiment made on a splenectomized rabbit by infusion homologous red cells of the same as well as different types, heterologous cells and trypsin treated auto-cells, made it clear that there was no significant difference in the loss curve of homologous cells, but that the losing speed of heterologous cells from the blood flow was extra rapid during the first 30 minutes of its infusion, and also that thereafter the loss curve was obtained almost in parallel with that of a normal rabbit.With regard to trypsin treated cells it was confirmed that its repeated infusion caused reduction of the loss curve and production of an auto-antibody, regardless of the presence or non-presence of spleen.The distribution of the infused normal auto-cells, trypsin treated auto-cells and heterologous cells was examined in heart blood, liver, spleen and kidney to find that the distribution of heterologous cells in the sysem was considerably different from that of homologous cells.Of the blood cells denatured by trypsin treatment, the homologous cells and the heterologous cells differ remarkably in the way they are destroyed and disposed, but the homologous cells which have been subjected to a powerful irreversible change are disposed in the liver in compensation of the spleen, same as heterolgoous cells.
著者
土谷 太郎 蔵田 元二 横山 三男 高橋 進
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.9, no.1, pp.18-24, 1962 (Released:2010-03-12)
参考文献数
23
被引用文献数
2

Various examples of the R0R0 (-D-/-D-) genotype have been found in England, U. S. A., Japan and Puerto Rico.The present paper reports studies of an additional family.Characteristics of these bloods as established by previous studies were also seen in our cases, and it seems to reveal a complex array of Rh antibodies.
著者
村井 弘之
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.47, no.3, pp.363-368, 2001-07-01 (Released:2010-03-12)
参考文献数
19
被引用文献数
1
著者
斎藤 健一 安武 幹智 芹澤 領 丸山 みどり 竹内 久彌 小尾 俊一 小畑 清一郎 浅野 茂隆 高橋 恒夫
出版者
一般社団法人 日本輸血・細胞治療学会
雑誌
日本輸血学会雑誌 (ISSN:05461448)
巻号頁・発行日
vol.44, no.1, pp.12-19, 1998-02-01 (Released:2010-03-12)
参考文献数
16
被引用文献数
1

Banking of human placental/umbilical cord blood (PCB) requires volume reduction for prevention of hemolysis-related side effects and cost-effective storage. We collected, separated, and cryopreserved PCB following the New York Blood Center's protocol, and obtained PCB with an mean volume of 70.0±28.1ml (n=100). Red cells were sedimented by addition of 1% hydroxyethyl starch with gentle centrifugation. Recovery of nucleated cells (NC) and colonyforming units (CFU) was 82.9±13.7% and 90.4±18.9%, respectively (n=25). Volume of the NC suspension was reduced to 20ml by centrifugation and 5ml of 50% dimethylsulfoxide and 5% Dextran 40 solution were added slowly. The frozen cells were washed with a solution containing 10% Dextran 40 and 5% human serum albumin. NC and CFU recovery was 88.8±7.13% and 81.2±20.3%, respectively (n=18). No aggregation of cells occurred after the washing procedure.We studied the effect of storage after collection, cell separation, and cryopreservation under various conditions. Results suggested that collected PCB should be prestored at 25°C for less than 24 hours, and that HES of moleculer weight 400, 000 should be used at the concentration of 1-4% for efficient cell separation. Further, the cryoprotective solution could maintain the number of NCs, CFUs, and CD34+ cells under slow cooling.