著者

宮田(古谷) 聡美 河野 惠三 森元 崇史 原島 哲 岩田 裕子 有安 利夫

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.142, no.5, pp.535-546, 2022-05-01 (Released:2022-05-01)

参考文献数

29

被引用文献数

1

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Transient receptor potential vanilloid 2 (TRPV2) channels are expressed and play functional roles in various immune cells. Physical stimuli leading to TRPV2 activation causes mast cell degranulation. Besides their roles in immune cells, it has been shown that TRPV2 channels are pathophysiologically relevant to degenerative muscular diseases such as dilated cardiomyopathy and muscular dystrophy. Hence, development of drug candidates that inhibit human TRPV2 activation is an urgent matter. NK-4, a cryptocyanine dye, inhibited agonist-induced TRPV2 activity in mouse TRPV2-transfected HEK293 cells. However, it remains unclear whether NK-4 exerts regulatory effects on the activation of human TRPV2 channels. In this study, we show that NK-4 inhibits intracellular Ca2+ increase in human TRPV2-transfected HEK293 cells preactivated with a TRPV2 agonist. The inhibitory effect of NK-4 (IC50=0.27 μM) on human TRPV2 activation was 74-fold stronger than that on mouse TRPV2 activation (IC50=20 μM). NK-4 also inhibited the agonist-induced TRPV2 expression at the plasma membrane, when the human TRPV2-expressing cells were stimulated with the agonist in the presence of NK-4. These results suggest that NK-4 abrogates the agonist-induced signaling events leading to human TRPV2 activation. Furthermore, TRPV2 agonist caused degranulation of RBL-2H3 cells, which represents a phenomenon related to physical urticarias. NK-4 suppressed the release of β-hexosaminidases upon degradation with IC50 of 1.9 μM, 35-fold lower than that determined with an anti-allergic drug, Epinastine. Our results suggest that NK-4 would be a potential therapeutic strategy to resolve dilated cardiomyopathy and its associated heart failure as well as physical urticarias.

著者

宮田(古谷) 聡美 河野 惠三 森元 崇史 原島 哲 岩田 裕子 有安 利夫

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

pp.21-00219, (Released:2022-02-28)

参考文献数

29

被引用文献数

1

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Transient receptor potential vanilloid 2 (TRPV2) channels are expressed and play functional roles in various immune cells. Physical stimuli leading to TRPV2 activation causes mast cell degranulation. Besides their roles in immune cells, it has been shown that TRPV2 channels are pathophysiologically relevant to degenerative muscular diseases such as dilated cardiomyopathy and muscular dystrophy. Hence, development of drug candidates that inhibit human TRPV2 activation is an urgent matter. NK-4, a cryptocyanine dye, inhibited agonist-induced TRPV2 activity in mouse TRPV2-transfected HEK293 cells. However, it remains unclear whether NK-4 exerts regulatory effects on the activation of human TRPV2 channels. In this study, we show that NK-4 inhibits intracellular Ca2+ increase in human TRPV2-transfected HEK293 cells preactivated with a TRPV2 agonist. The inhibitory effect of NK-4 (IC50 = 0.27 μM) on human TRPV2 activation was 74-fold stronger than that on mouse TRPV2 activation (IC50 = 20 μM). NK-4 also inhibited the agonist-induced TRPV2 expression at the plasma membrane, when the human TRPV2-expressing cells were stimulated with the agonist in the presence of NK-4. These results suggest that NK-4 abrogates the agonist-induced signaling events leading to human TRPV2 activation. Furthermore, TRPV2 agonist caused degranulation of RBL-2H3 cells, which represents a phenomenon related to physical urticarias. NK-4 suppressed the release of β-hexosaminidases upon degradation with IC50 of 1.9 μM, 35-fold lower than that determined with an anti-allergic drug, Epinastine. Our results suggest that NK-4 would be a potential therapeutic strategy to resolve dilated cardiomyopathy and its associated heart failure as well as physical urticarias.

著者

岩田 裕子

雑誌

人間と文化 (ISSN:24326399)

巻号頁・発行日

no.4, pp.254-263, 2021-03-31

著者

岩田 裕子 森 恵美 土屋 雅子 坂上 明子 前原 邦江

出版者

千葉看護学会

雑誌

千葉看護学会会誌 (ISSN:13448846)

巻号頁・発行日

vol.23, no.1, pp.71-79, 2017-08

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[抄録] 目的:日本では高年初産婦が珍しくなくなってきているが,これらの女性は産後うつに対して脆弱であることが示唆されている。本研究の目的は,産後1か月時に産後うつスクリーニング陽性である日本人高年初産婦の母親としての経験を記述することである。方法:本研究はケーススタディであり,2011年の6月から12月の期間に3つの病院で健康な単胎児を出産した21人の高年初産婦を対象とした。質的データと量的データを収集し,量的データはうつのハイリスク女性を抽出し,さらに質的データの補完的解釈に用いた。量的データとしては,1)アクティグラフを用いて測定した客観的睡眠の質と,2)日本語版エジンバラ産後うつ病自己調査票(EPDS)により測定したうつ症状の,2つのデータを収集した。日本語版EPDSで9点以上の得点者を,うつのハイリスク女性とした。うつのハイリスク女性の母親としての経験に関しては,半構成的面接によりデータ収集し,質的に分析した。ナラティブ統合により,個々の女性の文脈の中で質的データと量的データを解釈した。結果:うつのハイリスク女性は5名であった。本研究の結果から,高年初産婦の経験を理解するために重要な以下のテーマが抽出された。1)身体的健康状態の維持,2)子どもの世話:実践,気がかり,対処,3)ソーシャルサポートの利用,4)基本的ニーズの充足,5)新しい生活への適応。考察:母親個々の状況の中での母親としての経験を理解することが,適切なケア提供につながると考えられる。[ABSTRACT] Purpose: Older primiparae have become more common in Japan. It has been suggested these women are vulnerable to post-partum depression. The present study aimed to describe maternal experiences of older Japanese primiparae with a positive screen for depression at 1 month post-partum. Methods: This case study examined 21 older primiparae who delivered healthy singletons at three Japanese hospitals from June to December 2011. We used qualitative and quantitative data, with quantitative data for selecting women at high risk for depression as complementary to qualitative data. Quantitative data included: 1) objective sleep quality measured by actigraphy and 2) depressive symptoms measured by the Japanese version of the Edinburgh Postnatal Depression Scale (EPDS). Women who scored 9 or more on the EPDS were considered to be at high risk for depression. Semi-structured interviews were conducted to explore maternal experiences of women at high risk for depression and analysed qualitatively. Narrative integration was used by interpreting qualitative and quantitative findings in each woman's individualized context. Results: Five women were at high risk for depression. Our findings support the importance of understanding older primipara's experiences of: 1) maintaining physical well-being; 2) childcare: practice, concern, and coping; 3) utilizing social support; 4) meeting basic needs; and 5) adjustment to a new life. Discussion: Understanding maternal experiences in each individualized context will lead to providing appropriate care.

著者

岩田 裕子 森 恵美 Iwata Hiroko Mori Emi イワタ ヒロコ モリ エミ

出版者

千葉看護学会

雑誌

千葉看護学会会誌 (ISSN:13448846)

巻号頁・発行日

vol.10, no.1, pp.49-55, 2004-06-30

被引用文献数

3

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本研究の目的は,父親役割への適応を促す看護援助を明らかにすることであり,国内外の研究論文および専門書の内容を質的に分析することにより,父親役割への適応に関する看護援助を抽出した。医学中央雑誌,CINAHL,MEDLINEを利用したコンピューター検索により,1990年以降の文献を抽出し,最終的に和文献16,英語文献24,専門書9を分析対象とした。質的分析の結果,父親役割への適応を促す看護援助は,妊娠中に行う援助としては,1)アタッチメントの促進,2)父親役割への準備,3)夫婦間コミュニケーションの促進,4)役割調整のための夫婦間の話し合い促進,5)胎児・乳児についての情報提供の5つが抽出された。分娩時に行う援助は,父親になる男性へのサポートとしてまとめられ,妊婦だけでなく,父親になる男性にも焦点をおいた援助をすべきであるということが示された。出産後に行う援助としては,1)育児相談・教育,2)父親間での感情の共有,3)夫婦間コミュニケーションの促進の3つが抽出された。また援助を行う時期に関係なく抽出された一般的原理は,1)援助者の姿勢,2)物理的環境の調整,3)援助する際の工夫の3つであった。本研究において明らかとなった父親役割への適応を促す看護援助は,移行期の父親にケアを提供する看護実践者にとって,援助の指針となるものである。また,抽出された看護援助の内容をさらに検討あるいは精錬していくことは,日本文化を反映した新たな看護援助の指針を開発することにつながると考える。The purpose of this study was to clarify nursing care that would promote men's adjustment to fatherhood. Literature search was conducted to retrieve relevant articles written either in Japanese or English. Igakuchuouzasshi, CI-NAHL, and MEDLINE were searched since 1990. The results of 16 Japanese articles, 24 English articles, and 9 books were analyzed focusing on their content to extract nursing care that would promote men's adjustment to fatherhood. The analysis resulted in various nursing care that could be provided during pregnancy, labor, and after birth. Nursing care during pregnancy were: 1) promoting the expectant father's attachment, 2) preparing for the father's role, 3) promoting marital communication, 4) promoting marital communication for the adjustment of parental roles between the couple, and 5) providing necessary information about fetus and infant. Nursing care during labor could be categorized as providing support to the expectant/new father that emphasized men as clients. Nursing care after birth were: 1) consultation/education about parenting, 2) sharing feelings among fathers, and 3) promoting marital communication. The following three basic principles were also extracted: 1) attitudes of care providers, 2) arrangement of physical environments, and 3) consideration of care providers. The result could be a knowledge base to develop new nursing interventions that are sensitive to the Japanese culture and effective in promoting men's adjustment to fatherhood.