著者

カングスワン アタヤ 野口 玉雄 荒川 修 清水 潮 塚本 久美子 志田 保夫 橋本 周久

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.54, no.10, pp.1799-1802, 1988-10-25 (Released:2008-02-29)

参考文献数

11

被引用文献数

6 7

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Attempts were made to detect tetrodotoxin (TTX)-producing bacteria in the Thai horseshoe crab Carcinoscorpius rotundicauda, one of TTX-containing marine invertebrates. Thirteen dom-inant bacteria were isolated from the intestinal contents of live horseshoe crab specimens, and were cultured in a 2 × ORI medium at 20°C for 24h. Bacterial cells of each strain were harvested, ultrasonicated and ultrafiltered, and TTX and anh-TTX fractions were separated from the filtrate by Bio-Gel P-2 column chromatography. HPLC, UV and GC-MS analyses showed that all out of the 13 strains isolated produced anh-TTX, and one strain produced TTX and anh-TTX. The strain which produced both toxins was identified as Vibrio alginolyticus on the basis of biochemical and other characters.