著者

有竹修二著 昭和大蔵省外史刊行会編

出版者

昭和大蔵省外史刊行会

巻号頁・発行日

1967

著者

荒木 日出之助 藤川 雄平 黒沢 恒平 佐藤 勲 昭和大 昭和大 昭和大 昭和大

雑誌

日本産科婦人科學會雜誌 = Acta obstetrica et gynaecologica Japonica (ISSN:03009165)

巻号頁・発行日

vol.24, no.8, 1972-08-01

著者

野口 有生 平戸 久美子 斉藤 裕 高崎 克哲 矢内原 巧 中山 徹也 Yusei NOGUCHI Kumiko HIRATO Hiroshi SAITO Katsunori TAKASAKI Takumi YANAIHARA Tetsuya NAKAYAMA 昭和大学医学部産科婦人科学教室 昭和大学医学部産科婦人科学教室 昭和大学医学部産科婦人科学教室 昭和大学医学部産科婦人科学教室 昭和大学医学部産科婦人科学教室 昭和大学医学部産科婦人科学教室 Department of Obstetrics and Gynecology Showa University School of Medicine Department of Obstetrics and Gynecology Showa University School of Medicine Department of Obstetrics and Gynecology Showa University School of Medicine Department of Obstetrics and Gynecology Showa University School of Medicine Department of Obstetrics and Gynecology Showa University School of Medicine Department of Obstetrics and Gynecology Showa University School of Medicine

雑誌

日本産科婦人科學會雜誌 = Acta obstetrica et gynaecologica Japonica (ISSN:03009165)

巻号頁・発行日

vol.40, no.3, pp.359-364, 1988-03-01

被引用文献数

1

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分娩時ヒト子宮頚部組織における遊離アラキドン酸生成能と, それに及ぼす妊娠性ステロイドの影響を調べる目的で次の実験を行ない, 以下の成績を得た. 1) 正常分娩のヒト子宮頚部組織800g上清を酵素源とし, L-3-phosphatidylcholine, 1-stearoyl-2-[1-^<14>C]arachidonylを基質とし, インキュベーションを行ない, 遊離されたアラキドン酸産生量よりphospholipase A_2活性を測定し, 更に各種のkineticsを行ない, ヒト子宮頚部組織内でリン脂質より遊離アラキドン酸産生に介在するphospholipase A_2活性が存在することを示した. 2) 培養液中に妊娠中増加する各種の妊娠性ステロイド(cortisol, pregnenolone, 20α-dihydroprogesterone, pregnenolone-sulfate, DHA, DHA-sulfate, estrone, estradiol, estriol)を添加し, 本酵素活性に及ぼす影響を検討した. 各種妊娠性ステロイド添加では, 本酵素活性に対し, 明らかに影響を示すものはなかつた. 3) 分娩第1期に母体にDHA-sulfate (マイリス) 600mgを2時間で点滴静注した後, 分娩に至つたDHA-sulfate投与群と非投与正常分娩例との本酵素活性の比較では, 投与群40±13pmoles/mg protein であり, 非投与正常分娩例の39±7pmoles/mg proteinと比し差はみられなかつた. 以上のことよりヒト妊娠子宮頚部に, phospholipase A_2活性が存在することが明らかとなり, また, 本実験条件下ではprostaglandin合成機構の一過程であるphospholipase A_2活性に対する妊娠性ステロイドの関与は認められなかつた.Prostaglandins (PGs) play an important role in cervical ripening. It is known that the hydrolytic release of arachidonic acid from phospholipids regulates the rate of PG formation. To study the PG biosynthesis in cervical tissue, phosphatidylcholine containing ^<14>C-arachidonic acid in Sn-2 position was incubated with the 800 × g supernatant of cervical tissue obtained from pregnant women at delivery. The only recognizable radiolabeled metabolite, ^<14>C-arachidonic acid, was found on an autor-adiogram of TLC, which corresponded to authentic arachidonic acid. Therefore, phospholipase A_2 activity was calculated as the rate of the release of arachidonic acid from phosphatidylcholine under the conditions used. The optimal pH of phospholipase A_2 activity in 800 × g supernatant was found to be 7.0. It was found that the addition of Ca^<2+> increased the enzyme activity. It was demonstrated that the concentrations of DHA-sulfate (DHA-S) and conjugated estrogens were higher in ripened cervical tissue than in non-ripened tissue and that PGI_2 and PGE_2 production increased following the addition of DHA-S. The effects of steroids mainly derived from feto-placental unit, cortisol, pregnenolone, 20α-dihydroprogesterone, pregnenolone-sulfate, DHA, DHA-S, estrone, estradiol and estriol on arachidonic acid release were also studied in vitro. After the onset of labor, DHA-S was administered to the patients in vivo and their cervical tissues were collected at delivery. It was found that steroids including DHA-S did not affect phospholipase A_2 activity under the conditions used. These results indicate that cervical tissue posseses the ability to release arachidonic acid from phospholipid, although this step in PG formation might not be affected by steroids including DHA-S.

著者

中村 靖 斉藤 茂 山崎 健一 柴崎 好伸 Yasushi NAKAMURA Shigeru SAITO Kenichi YAMASAKI Yoshinobu SHIBASAKI 昭和大学歯学部歯科矯正学教室 昭和大学歯学部歯科矯正学教室 昭和大学歯学部歯科矯正学教室 昭和大学歯学部歯科矯正学教室 Department of Orthodontics School of Dentistry Showa University Department of Orthodontics School of Dentistry Showa University Department of Orthodontics School of Dentistry Showa University Department of Orthodontics School of Dentistry Showa University

雑誌

日本矯正歯科学会雑誌 = The journal of Japan Orthodontic Society (ISSN:0021454X)

巻号頁・発行日

vol.54, no.3, pp.159-169, 1995-06

参考文献数

47

被引用文献数

4

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矯正学的歯の移動時には, 歯槽骨を形成している細胞と歯根膜細胞が矯正力というmechanical stressに対してさまざまな反応を示すことが知られている.そこで本研究は同一外科矯正患者から同時に採取した下顎骨片と健全な下顎第一小臼歯から骨系細胞と歯根膜細胞を剥離して培養した.両細胞に間欠的遠心力(50&acd;250回転)を付与し, DNA合成能, 細胞内アルカリフォスファターゼ(ALPase)活性, サイクリックAMP(cAMP)産生量, プロスタグランディンE_2(PGE_2)産生量, インターロイキン6(IL-6)産生量, 骨吸収能を測定した.両細胞に付与した間欠的遠心力が上記測定項目に対してどのような影響を与えるかを検討し, あわせて両細胞の反応性の相違についても比較検討した.その結果, 1. DNA合成能および細胞内ALPase活性は, 250回転において両細胞とも有意に促進された.2. 骨系細胞において, 遠心力の付与によりすべての回転数でcAMPおよびPGE_2産生量が有意に抑制され, IL-6産生量に関しても50回転において抑制傾向があった.3. 歯根膜細胞においては, 遠心力を付与してもcAMP, PGE_2, IL-6の産生量がほとんど変化しなかった.4. 骨吸収能は遠心力を付与した培養上清を用いると両細胞とも150回転で有意に抑制された.以上のことから, DNA合成能と細胞内ALPase活性は高回転の間欠的遠心力に対して両細胞とも促進することが示された.また, 骨系細胞の骨吸収能はPGE_2, IL-6の影響を直接的に受けやすいが, 歯根膜細胞に関してはPGE_2, IL-6以外にも骨吸収能に影響を与える何らかの因子が存在することも示唆された.It has been shown that bone cells and periodontal ligament (PDL) cells have various responses to mechanical stress during orthodontic tooth movement. The following experiments were performed to compare the responses of these two cell types to mechanical stress. Cortical bone specimens derived from mandible and PDL of first premolars were obtained at the same time from the same patient undergoing surgical orthodontics. In the confluent phase, intermittent centrifugal forces (ICF) were applied to the cultured bone cells and PDL cells at 50&acd;250 rpm. After centrifugation, the cultured cells were used for the assays of DNA synthesis, alkaline phosphatase (ALPase) activity and cyclic AMP (cAMP) production, and the cultured media were used for the assays of prostaglandin E_2 (PGE_2) production, interleukin-6 (IL-6) production and bone resorption activity. The results were as follows : 1. ICF at 250 rpm significantly induced DNA synthesis and ALPase activity in each cell type. 2. In bone cells, the amounts of cAMP and PGE_2 were significantly decreased by ICF at all magnitudes of force tested, and those of IL-6 tended to be decreased by ICF at 50 rpm. 3. In PDL cells, the amounts of cAMP, PGE_2 and IL-6 were hardly changed by ICF at all magnitudes of force tested. 4. ICF at 150 rpm significantly inhibited the bone resorption activity in each cell type. These results suggested that DNA synthesis and ALPase activity are stimulated by high-speed ICF in each cell type. These results also suggested that bone resorption activity caused by ICF in bone cells was directly affected by PGE_2 and IL-6. However, in PDL cells, there may be agents that affect bone resorption activity other than PGE_2 and IL-6.