- 著者
- 大橋 一智 上田 浩史 山崎 正利 木村 貞夫 安部 茂 山口 英世
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- 藥學雜誌 (ISSN:00316903)
- 巻号頁・発行日
- vol.112, no.12, pp.919-925, 1992-12-25
- 被引用文献数
- 19
The biological activity of a preparation of heat killed cells of Enterococcus faecalis, FK-23 which was isolated from the feces of a healthy human, was investigated in C3H/He mice. Intraperitoneal injection of the preparation caused an accumulation of neutrophils and macrophages in the peritoneal cavity of the mice 6 h later. As a parameter of the activation of macrophages, the effect of the FK-23 preparation on the production of tumor necrosis factor (TNF) was examined. The mice were given two consecutive intravenous injections of the preparation at a dose of 10μg/mouse and, 3 h later, of 300μg/mouse. The TNF level in the sera reached 99 U/ml in mice 2 h after the second injection. This preparation also stimulated peritoneal macrophages to produce TNF in vitro and increased the capacity of neutrophils to adhere to plastic plates and to release active oxygens, but did not induce blastogenic transformation of lymphocytes. These results suggest that the FK-23 preparation is a biological response modifier (BRM) with various activities on phagocytes similar to a streptococcal antitumor agent, OK432.
- 著者
- 大橋 一智 上田 浩史 山崎 正利 木村 貞夫 安部 茂 山口 英世
- 出版者
- 公益社団法人 日本薬学会
- 雑誌
- YAKUGAKU ZASSHI (ISSN:00316903)
- 巻号頁・発行日
- vol.112, no.12, pp.919-925, 1992-12-25 (Released:2008-05-30)
- 参考文献数
- 18
- 被引用文献数
- 10 10
The biological activity of a preparation of heat killed cells of Enterococcus faecalis, FK-23 which was isolated from the feces of a healthy human, was investigated in C3H/He mice. Intraperitoneal injection of the preparation caused an accumulation of neutrophils and macrophages in the peritoneal cavity of the mice 6 h later. As a parameter of the activation of macrophages, the effect of the FK-23 preparation on the production of tumor necrosis factor (TNF) was examined. The mice were given two consecutive intravenous injections of the preparation at a dose of 10μg/mouse and, 3 h later, of 300μg/mouse. The TNF level in the sera reached 99 U/ml in mice 2 h after the second injection. This preparation also stimulated peritoneal macrophages to produce TNF in vitro and increased the capacity of neutrophils to adhere to plastic plates and to release active oxygens, but did not induce blastogenic transformation of lymphocytes. These results suggest that the FK-23 preparation is a biological response modifier (BRM) with various activities on phagocytes similar to a streptococcal antitumor agent, OK432.
1 0 0 0 サツマイモ黒斑病防除薬剤のスクリーニング法
(1) サツマイモ黒斑病に対する薬剤の防除効果を検定するために,厚さ7~10mmのサツマイモ・スライスを所定濃度の供試薬液に浸漬, 風乾後, 黒斑病菌を接種し,薬剤の効果を調査した。<BR>(2) 本法により,高い抗菌力のみられたベンレート水和剤500倍,トップジン500倍および,抗菌力の低くかったウスプルン800倍処理によりほ場試験を行なった。その結果,ベンレートの接種2時間前および2時間後処理の防除効果は顕著であったが,24時間後処理では効果の低下がみられた。ウスプルンとトップジン処理の効果は低くかった。<BR>(3) 試験結果から,サツマイモ・スライスを用いた簡易検定法により抗菌力の低い薬剤を除外することができ,有効薬剤についてのみほ場試験を行なってその効果を確認すればよく,本法は,簡易で実用的である。
- 著者
- 大橋 一智 里中 勝人 山本 哲郎 山崎 正利 木村 貞夫 安部 茂 山口 英世
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- 藥學雜誌 (ISSN:00316903)
- 巻号頁・発行日
- vol.113, no.5, pp.396-399, 1993-05-25
- 被引用文献数
- 10
The antitumor activity of a preparation of heat-killed cells of Enterococcus faecalis, FK-23. Intraperitoneal injection of the preparation prolonged the lifespan of C3H/He N mice which were intraperitoneally inoculated with MM46 mammary carcinoma. Not only intraperitoneal but also oral administration of the FK-23 preparation inhibited the growth of these carcinomas inoculated intradermally. The tumor-bearing mice produced tumor necrosis factor (TNF) in their sera 2 h after intravenous injection of OK432. This TNF level increased after the mice were fed with food supplemented with the FK-23 preparation. Additionally, the FK-23 preparation inhibited the growth of Meth A fibrosarcoma in cyclophosphamide-treated BALB/c mice.