- 著者
-
山野上 祐介
馬渕 浩司
澤井 悦郎
坂井 陽一
橋本 博明
西田 睦
- 出版者
- 一般社団法人 日本魚類学会
- 雑誌
- 魚類学雑誌 (ISSN:00215090)
- 巻号頁・発行日
- vol.57, no.1, pp.27-34, 2010 (Released:2014-03-05)
- 参考文献数
- 22
Morphological identification of two ocean sunfish species (genus Mola) is not an easy task due to the giant body size and poor understanding of their intraspecific variation. A rapid, reliable method of identifying mitochondrial DNA from two Mola species in Japanese waters was designed for PCR-based genotyping of the mitochondrial control region. Two allele-specific primers were developed for the mitochondrial control region of each species: CRMolaAL and CRMolaBL for Mola spp. A and B, respectively. Using the same reverse primer (H884-12S), specific primers for Mola spp. A and B were designed to amplify ca. 400- and 500-bp fragments, respectively. A pair of fish universal primers (L1969- 16S and H2582-16S) amplifying ca. 700 bp was used as an internal control. Multiplex PCR reactions including these five primers produced a species-specific fragment for either Mola sp. A or B with an internal control. This approach has major advantages over other molecular species-identification methods in speed and costefficiency, and greatly helps in determining Mola species.