著者

由良 敬

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.51, no.3, pp.144-145, 2011 (Released:2011-05-25)

著者

由良 敬

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.128, no.11, pp.1547-1555, 2008-11-01 (Released:2008-11-01)

参考文献数

28

被引用文献数

2 2

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A vast amount of DNA sequence data, protein three-dimensional (3D) structure data, and RNA expression data have been produced by the efforts of genome sequencing, structural genomics, and omics projects, and we are at the stage where comprehensive views of cell activity and molecular mechanisms of life can be deduced. But in reality, we are inundated with massive amounts of data and are still in the process of finding ways to fully utilize the data. In this report, I would like to present our observations on the growth of protein 3D structure data and our effort to deduce the functions from the 3D structures. We found that the 3D structure of quite a high proportion of proteins derived from genome sequences can be now predicted and methods to predict the functions from 3D structures are in high demand. The methods we have developed can be used to predict some functions, namely RNA and ligand interfaces, based on those 3D structures and DNA sequences with relatively high accuracy. The methods enable predictions that are accurate enough to help with deducing the atomic structures of the complexes.

著者

鳴海 一成 黒木 良太 由良 敬

出版者

独立行政法人日本原子力研究開発機構

雑誌

基盤研究(B)

巻号頁・発行日

2007

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放射線抵抗性細菌デイノコッカス・ラジオデュランス由来のDNA修復促進タンパク質PprAについて、1.35Åの分解能での結晶構造解析に成功し、DNA結合に重要なアミノ酸残基の空間配置を明らかにした。また、PprAタンパク質の放射線誘導に関わる新規制御タンパク質PprMを同定し、その作用機序を明らかにした。さらに、デイノコッカス属細菌で使用できる新規プラスミドベクターを開発した。