著者

片町 太輔 池田 実 董 仕 谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.77, no.1, pp.31-39, 2011 (Released:2011-03-18)

参考文献数

42

被引用文献数

1 1 2

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中国の長江から利根川流域に導入されたハクレン集団の遺伝的多様性を検討するため,成魚,流下卵,仔魚の計 56 個体を対象に mtDNA 調節領域(454 bp)のシーケンス分析を行った。その結果,26 種類のハプロタイプが検出され,ハプロタイプ多様度(h)は 0.920,塩基多様度(π)が 0.011 であった。これらの値と既報の長江中下流域の集団の値との間には大きな差異はなく,ハプロタイプの系統関係も近縁であった。これらの結果から,利根川集団が長江の中下流域を起源としており,導入後も大きな有効集団サイズによって再生産を繰り返していることが示唆された。

著者

董 仕 大原 健一 谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.63, no.2, pp.201-206, 1997-03-15 (Released:2008-02-29)

参考文献数

21

被引用文献数

12 12

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The eggs of a Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperms of a common carp Cyprinus carpio. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: Hae III; probe: YNZ 22) in the offsprings were identical with maternal fish.The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.

著者

董 仕 谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.62, no.6, pp.891-896, 1996-11-15 (Released:2008-02-29)

参考文献数

18

被引用文献数

10 10

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The eggs of Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperm of a Gengoroubuna Carassius cuvieri and a commom carp Cyprinus carpio respectively. DNA extracted from blood of parents and offsprings were amplified using 20 random primers and separated by electrophoresis (RAPD-PCR). Isozymes of muscle and liver of parents and offsprings were assayed by the horizontal starch gel electrophoresis. Distinct differences by maternal fish were observed in the patterns of both RAPD-PCR (17 primers) and isozymes (CK, EST, GPI, PGM), but those of offsprings produced by crossing with Gengoroubuna and common carp respectively were the same completely within the maternal fish. Therefore, the results obtained in this study verified that the offsprings from Ginbuna were clonal genetically, being the same with maternal fish throughout the genome.

著者

董 仕 谷口 順彦 石田 力三

出版者

日本魚類学会

雑誌

魚類学雑誌 (ISSN:00215090)

巻号頁・発行日

vol.46, no.2, pp.83-90, 1999-11-30 (Released:2010-06-28)

参考文献数

18

被引用文献数

2

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Loaches, Misgurnus anguillicaudatus, collected from Torentsu river, Ibaraki, Japan, were found to comprise two types based on the morphological and genetic traits. Complete substitution of alleles of the two types at the AAT-2* and GPI-1* loci were found, as well as significant differences in allele frequencies at eight other loci. The genetic distance between the two types was 0.4. The belly color was yellowish in type I and grayish in type II, and the average number of precurrent rays was 21.4 (range: 16-25) and 10.5 (range: 7-13), respectively.The results suggested that the two types should be regarded as a distinct biological species.