著者

瀧川 由宇登 森 勇人 関 伸吾 小松 章博 谷口 順彦

出版者

Japanese Society for Aquaculture Science

雑誌

水産増殖 (ISSN:03714217)

巻号頁・発行日

vol.42, no.3, pp.477-483, 1994-09-20 (Released:2010-03-09)

参考文献数

11

---

マダイにおける第一卵割阻止型雌性発生二倍体 (mitotic-G2n) 誘導のための最適条件の検討を行った。染色体の倍数化は高水圧処理法を用いた。高水圧処理の媒精後処理開始時間, 処理水圧, 処理時間の組み合わせにより条件検討を行いそれぞれの試験区でビーカー試験により孵化率, 正常二倍体孵化率, および半数体孵化率を求めた。mitotic-G2nの誘導に最適と考えられる条件は高水圧処理の媒精後処理開始時間45分, 処理水圧700kg/cm2, 処理時間5分であり, この場合の孵化率は35.06%, 正常二倍体孵化率は53.41%, 半数体孵化率は46.59%であった。しかし, 異数体と思われる個体の出現が多く変態期は大量へい死もみられたことから, さらに最適条件の検討を重ねる必要性が示唆された。

著者

片町 太輔 池田 実 董 仕 谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.77, no.1, pp.31-39, 2011 (Released:2011-03-18)

参考文献数

42

被引用文献数

1 1 2

---

中国の長江から利根川流域に導入されたハクレン集団の遺伝的多様性を検討するため,成魚,流下卵,仔魚の計 56 個体を対象に mtDNA 調節領域(454 bp)のシーケンス分析を行った。その結果,26 種類のハプロタイプが検出され,ハプロタイプ多様度(h)は 0.920,塩基多様度(π)が 0.011 であった。これらの値と既報の長江中下流域の集団の値との間には大きな差異はなく,ハプロタイプの系統関係も近縁であった。これらの結果から,利根川集団が長江の中下流域を起源としており,導入後も大きな有効集団サイズによって再生産を繰り返していることが示唆された。

著者

GONZALES Benjamin J. 岡村 収 谷口 順彦

出版者

水産増殖談話会

雑誌

水産増殖 (ISSN:03714217)

巻号頁・発行日

vol.44, no.1, pp.7-15, 1996-03-20

参考文献数

19

被引用文献数

4

著者

董 仕 大原 健一 谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.63, no.2, pp.201-206, 1997-03-15 (Released:2008-02-29)

参考文献数

21

被引用文献数

12 12

---

The eggs of a Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperms of a common carp Cyprinus carpio. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: Hae III; probe: YNZ 22) in the offsprings were identical with maternal fish.The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.

著者

董 仕 谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.62, no.6, pp.891-896, 1996-11-15 (Released:2008-02-29)

参考文献数

18

被引用文献数

10 10

---

The eggs of Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperm of a Gengoroubuna Carassius cuvieri and a commom carp Cyprinus carpio respectively. DNA extracted from blood of parents and offsprings were amplified using 20 random primers and separated by electrophoresis (RAPD-PCR). Isozymes of muscle and liver of parents and offsprings were assayed by the horizontal starch gel electrophoresis. Distinct differences by maternal fish were observed in the patterns of both RAPD-PCR (17 primers) and isozymes (CK, EST, GPI, PGM), but those of offsprings produced by crossing with Gengoroubuna and common carp respectively were the same completely within the maternal fish. Therefore, the results obtained in this study verified that the offsprings from Ginbuna were clonal genetically, being the same with maternal fish throughout the genome.

著者

畑中 宏之 稲田 善和 谷口 順彦

出版者

水産増殖談話会

雑誌

水産増殖 (ISSN:03714217)

巻号頁・発行日

vol.39, no.3, pp.241-247, 1991

---

低水温ショック法または高水圧ショック法により, アユの人為三倍体を作出し, 正常二倍体と比較しながら三倍体の成長および成熟における特性の解明を試みた。未成魚期の成長および飼料効率については三倍体がやや劣る傾向が認められた。適性な飼育条件の検討の必要性が示唆された。成熟が始まると, 二倍体が成長の停滞を示し, 産卵期が終わると死亡するのに対し, 三倍体の雌魚は越年後も死亡することなく順調に成長した。

著者

谷口 順彦 森田 敏夫

出版者

日本水産學會

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.45, no.1, pp.37-41, 1979

被引用文献数

1 4

---

Lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) of three related freshwater eels, <i>Anguilla anguilla, A. rostrata</i>, and <i>A. japonica</i> were examined by starch-gel electrophoresis using a citrate-aminopropylmorpholin buffer adjusted to pH 6.0. Five equal-spaced major bands were observed in LDH zymograms of the adult muscle exudation and the whole elver homogenate through the three species. The most anodal band was identified as the homotetramer of B subunits (B₄), and the most cathodal as the homotetramer of A subunit (A₄), based on the specificity of tissue distribution of these isozymes. These A₄, and B₄ bands of the Japanese eel were different from those of the European and American eels in electrophoretic mobility. In the MDH zymograms of muscle, four major bands were observed in all the three species. One of these, the activity of which was especially intense with the liver, was identified as A₂. The other two equal-spaced major bands of muscle were identified as AB and B₂, and the remaining most cathodal one as C₂. The European eel was distinguished from the American and Japanese eels by the A₂ band. The Japanese eel was also distinguished from the other species by the B₂ band. Although some variants were found in the LDH and MDH isozyme patterns of the respective species, their incidences were very low, and any alleles of these variants were not common among the three species.

著者

高木 基裕 谷口 順彦

出版者

水産育種研究会

雑誌

水産育種 (ISSN:13437917)

巻号頁・発行日

vol.31, no.2, pp.87-90, 2002-03

著者

谷口 順彦 岡田 容典

出版者

The Ichthyological Society of Japan

雑誌

魚類学雑誌 (ISSN:00215090)

巻号頁・発行日

vol.31, no.2, pp.181-187, 1984-08-01 (Released:2010-06-28)

参考文献数

10

---

Young of giant sciaenid, Argyrosomus japonicus (Temminck et Schlegel), were collected from coastal water of Kochi Prefecture, Japan. The young specimens, 18.0 to 44.9cm in total length, were compared morphologically and electrophoretically with adults, 72.5 to 135.0cm.Some morphological characters of the young did not agree with those of the adult described by a few researchers. It was ascertained that the proportions of head, orbit, and fin ray length changed gradually in relation with growth. Internal morphological characters, such as sagitta, diverticulum of the air-bladder, interorbital septum of neurocranium, show also changes in their shape. The form of caudal fin, which was one of the most important diagnostic characters of this species, also changed continuously from cuneate to truncate. These morphological changes seemed to be the reason why the young had never been recorded until now. There is a possibility that Nibea mi-ichthioides (Chu, Lo et Wu, 1963) which was synonymized with Argyrosomus amoyensis (Bleeker) by Trewavas (1977), may be the young of this species.To examine genetic identity of the young and adult specimens, the isozymes of muscle and liver were separated by starch-gel electrophoretic method. All of the alleles examined in the young well accorded with those of the adult.

著者

董 仕 谷口 順彦 石田 力三

出版者

日本魚類学会

雑誌

魚類学雑誌 (ISSN:00215090)

巻号頁・発行日

vol.46, no.2, pp.83-90, 1999-11-30 (Released:2010-06-28)

参考文献数

18

被引用文献数

2

---

Loaches, Misgurnus anguillicaudatus, collected from Torentsu river, Ibaraki, Japan, were found to comprise two types based on the morphological and genetic traits. Complete substitution of alleles of the two types at the AAT-2* and GPI-1* loci were found, as well as significant differences in allele frequencies at eight other loci. The genetic distance between the two types was 0.4. The belly color was yellowish in type I and grayish in type II, and the average number of precurrent rays was 21.4 (range: 16-25) and 10.5 (range: 7-13), respectively.The results suggested that the two types should be regarded as a distinct biological species.

著者

高木 基裕 谷口 順彦

出版者

日本水産増殖学会

雑誌

水産増殖 (ISSN:03714217)

巻号頁・発行日

vol.40, no.3, pp.329-333, 1992-09-20 (Released:2010-03-09)

参考文献数

4

被引用文献数

1

---

高知県内のカマキリの分布調査を行った。奈半利川, 伊尾木川, 安芸川, 四万十川, 小名鹿川, 立石川, 布川, 鍵掛川, 以布利川では潜水目視観察によってカマキリの生息が確認された。野根川, 安田川, 物部川, 仁淀川では聞き込み調査によって生息を確認した。カマキリの分布は堰堤の存在によってその直下域に限定され, 堰堤の上流では生息が確認できなかった。1980年の高知県の淡水魚類相調査と比較して, 高知県のカマキリの生息状況はさらに悪化しているものと考えられる。

著者

谷口 順彦

出版者

公益社団法人 日本水産学会

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.73, no.6, pp.1125-1128, 2007 (Released:2007-11-26)

参考文献数

6