著者
今村 博臣 安東 友美 藤川 誠
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.53, no.1, pp.020-023, 2013 (Released:2013-01-29)
参考文献数
8

ATP is indispensable for all living organisms. Despite its importance, however, distribution and dynamics of ATP level inside living cells still remained unclear. We have developed FRET-based ATP biosensors, “ATeam” and “GO-ATeam”, which enabled us to visualize intracellular ATP level. Using these biosensors, we have successfully evaluated the effect of IF1 protein and Ca2+ on regulation of ATP level. Also, we found that replication of hepatitis C virus genome resulted in the declination of cytosolic ATP level of host cells and the formation of putative virus replication sites with high ATP levels.