2 0 0 0 OA 大阪周辺に飛来するツバメの腸内細菌の群集構造解析
- 著者
- 見坂 武彦 片岡 憲司 藤光 隆司 谷 佳津治
- 出版者
- 公益社団法人 日本薬学会
- 雑誌
- YAKUGAKU ZASSHI (ISSN:00316903)
- 巻号頁・発行日
- vol.138, no.1, pp.117-122, 2018 (Released:2018-01-01)
- 参考文献数
- 21
- 被引用文献数
- 5
Migratory birds are considered as vectors of infectious diseases, owing to their potential for transmitting pathogens over large distances. The populations of barn swallow (Hirundo rustica) migrate from Southeast Asia to the Japanese mainland during spring and migrate back to Southeast Asia during autumn. This migratory population is estimated to comprise approximately hundreds to thousands of individuals per year. However, to date, not much is known about the gastrointestinal microbiota of the barn swallow. In this study, we characterized the fecal bacterial community in barn swallow. Using 16S rRNA gene metagenomic sequencing analysis, we examined the presence and composition of potentially pathogenic bacteria in the fecal samples, which were collected during spring season from Osaka. The number (±S.D.) of total bacteria was approximately 2.1(±3.4)×108 per gram of feces. In most samples, the bacterial community composition was dominated by families, such as Enterobacteriaceae, Pseudomonadaceae, Mycoplasmataceae, Enterococcaceae, Streptococcaceae, and Alcaligenaceae. However, no relationship was found between the bacterial community composition and geographical area in the fecal samples. Potentially pathogenic bacteria were detected at the rate of >0.1%, which included Pseudomonas spp., Escherichia/Shigella spp., Enterobacter spp., Yersinia spp., Mycoplasma spp., Enterococcus spp., Achromobacter spp., and Serratia spp. Our results suggested that barn swallow is instrumental in the transmission of these genera over large distances.
1 0 0 0 OA 渡り鳥飛来にともなう細菌群集の越境移動に関する環境微生物学的研究
細菌の越境移動に関する現状の把握は、健康・衛生の観点から重要な課題である。地球上の種々の細菌が渡り鳥とともに国境を越えて移動している可能性があるがその実態は明らかではない。本研究では、渡り鳥の糞に含まれる細菌群集を培養に依存せずに高速シーケンサーを用いて明らかにし、潜在的な病原細菌が長距離移動する実態を検証した。関西地区および北海道東部の沿岸部において、ツバメ科、カモ科、カモメ科の渡り鳥を主な対象として、糞に含まれる腸内細菌群集の16S rRNA遺伝子の配列を網羅的に解析し、鳥の種類による相違、他の生物との相違を明らかにした。また糞に含まれる多剤耐性菌について遺伝学的特徴を明らかにした。