著者
野口 智弘 村木 紀之 高野 克己 鴨居 郁三
出版者
Japan Association of Food Preservation Scientists
雑誌
日本食品保蔵科学会誌 (ISSN:13441213)
巻号頁・発行日
vol.23, no.1, pp.21-26, 1997-01-25 (Released:2011-05-20)
参考文献数
9

トリプシンおよびキモトリプシンの低温における活性は, 2-プロパノールやエタノール添加によって増加した。特に2-プロパノール添加では40℃付近で活性が低下したのに対し, 10℃付近の低温域では活性が増加し, また2一プロパノール添加によって温度依存性の低下がみられた。また, 酵素表面の疎水性は2-プロパノール添加によって増加し酵素の表面構造に変化が生じたことが示唆さ礼2一プロパノールが酵素タンパク質の構造を変化させることにより, 低温における活性の賦活効果を示すものと思われる。
著者
野口 智弘
出版者
日本食生活学会
雑誌
日本食生活学会誌 (ISSN:13469770)
巻号頁・発行日
vol.26, no.3, pp.103-109, 2015 (Released:2016-02-02)

Flour can be used to form dough with very high viscoelasticity by kneading well and adding water. The higher protein content in wheat flour helps form good dough that is suitable for making bread. However, there is the wheat which bread characteristics have bad though a protein content is high. In addition to the amount of gluten, the quality of the gluten has also been determined to be important. An increased interest in gluten quality has led to elucidation of the relationship between the sub-unit structures of gliadin and glutenin, and the baking quality. These studies have clearly shown that wheat containing “5+10” high molecular weight glutenin subunit (HMWG) exhibits excellent bread-making properties. Much of the wheat grown in North America and Europe contained “5+10”, but in Japan this was only true for a few varieties. In recent years, varieties with “5+10” have started to be made in Japan, introduced by breeding. The details of the mechanism of disulfide-binding (S-S bond) formation have not yet been determined. Protein disulfide isomerase (PDI) is the enzyme that catalyzes S-S bond formation in proteins. We think that PDI involved in wheat seed storage protein synthesis remains present in the flour, becoming involved in S-S bond formation in the bread-making process. We consider this hypothesis. As a result of PDI activity comparison of in flour, they tends to exhibit high PDI activity in bread making highly wheat was observed. As a result of the production of bread is added to the medium flour and PDI obtained by the E. coli expression system, it was found that improves the specific volume. In the future, by advancing the research on the relationship with the flour subunit structures and S-S bond formation by PDI, it is considered that meaningful information is obtained.
著者
岡 大貴 入澤 友啓 野口 智弘 内野 昌孝 岡田 早苗 高野 克己
出版者
日本食品保蔵科学会
雑誌
日本食品保蔵科学会誌 = Food preservation science (ISSN:13441213)
巻号頁・発行日
vol.37, no.3, pp.121-125, 2011-05-31

せんだんごは,対馬において伝統的な手法で,サツマイモから製造された澱粉である。せんだんごは'ろくべえ'という麺をはじめ,主食から間食まで幅広く加工利用されている。せんだんごは澱粉が約90%,繊維質が約6%を占め,SEMの結果,繊維質によって複数の澱粉粒が固まりとして存在していた。さらに,その繊維質はサツマイモ澱粉単独では形成できない生地のつなぎとなり,せんだんごより調製したろくべえは,冷麺やうどんとは異なるテクスチャーを示した。