著者

藤沢 延行 Adrian Ronald J.

出版者

一般社団法人日本機械学会

雑誌

日本機械学會論文集. B編 (ISSN:03875016)

巻号頁・発行日

vol.63, no.607, pp.818-823, 1997-03-25

被引用文献数

8

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A new calibration technique of liquid crystal thermometry has been introduced to the quantitative measurement of temperature in a thermal fluid and applied to the study of turbulent thermal convection. The present calibration technique improves the accuracy of temperature measurement in comparison with the classical calibration technique with hue. Three-dimensional temperature measurement of turbulent thermal convection over a horizontal smooth surface is carried out at a flux Rayleigh number of 3×10^9 by scanning a light sheet normal to the image plane and capturing a number of sequential visualized images. The spatial temperature distributions of thermal plumes over a horizontal surface are demonstrated by the reconstruction of isothermal contour surfaces and various cross-sectional views of the plumes. These results indicate the presence of a polygonal cell structure close to the surface and the generation of plumes at the intersections of high-temperature lines.

著者

Adrian Patalinghug YBAÑEZ Thillaiampalam SIVAKUMAR Badgar BATTSETSEG Banzragch BATTUR Khukhuu ALTANGEREL Kotaro MATSUMOTO Naoaki YOKOYAMA Hisashi INOKUMA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0361, (Released:2012-11-13)

被引用文献数

4 37

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Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA fragments of an Anaplasma sp. closely related to A. ovis (with 95.0% identity) were detected using a different nPCR method based on groEL gene. The phylogenetic analyses based on the Msp5, groEL and 16S rRNA genes demonstrated that A. marginale isolates in Mongolia were not divergent from the isolates distributed in other countries. The present study successfully established a new nPCR assay that can detect A. marginale, and reported the first molecular detection and characterization of A. marginale and an Anaplasma sp. closely related to A. ovis in Mongolian cattle populations.

著者

大野 健一 大野 泉 細野 昭雄 上江洲 佐代子 川端 望 木村 福成 ALTENBURG Tilman LEFTWICH Adrian KHAN Mushtaq GEBRE-AB Newai GEBREHIWOT Berihu Assefa 森 純一 PHAM Hong Chuong NGUYEN Thi Xuan Thuy

出版者

政策研究大学院大学

雑誌

基盤研究(A)

巻号頁・発行日

2010-04-01

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市場経済やグローバル化と矛盾しない産業振興を「プロアクティブな産業政策」と定義し、東アジアを中心にそのような政策事例を収集・比較したうえで、その具体的な内容、つくり方、組織、文書などを解説する英文・和文の書物を出版した。また研究成果を現実の開発政策に適用するために、本学が国際協力機構(JICA)等と共同で実施しているエチオピア政府およびベトナム政府との産業政策対話において、カイゼン、官民協力、行動計画のつくり方などにつき提言を行い、そのいくつかは実際に採用された。