著者
Yagame Takahiro Yamato Masahide
出版者
Springer Japan
雑誌
Journal of plant research (ISSN:09189440)
巻号頁・発行日
vol.126, no.2, pp.215-222, 2013-03-01
被引用文献数
11

Mixotrophy, obtaining carbon by mycoheterotrophy and photosynthesis, has been suggested in Cephalanthera species (Orchidaceae) by analyses on stable isotopes of carbon. In this study, we examined the growth of Cephalanthera falcata in pot cultured tripartite symbioses with Thelephoraceae fungi and Quercus serrata. Mycorrhizal fungi were isolated from roots of C. falcata in natural habitats. Two fungal isolates identified as Thelephoraceae were cultured and inoculated to fine roots of non-mycorrhizal seedlings of Q. serrata (Fagaceae). After the ectomycorrhizal formation, non-mycorrhizal seedlings of C. falcata were co-planted. The pots with tripartite symbioses were cultured in greenhouse for 30 months, and growth of C. falcata seedlings was examined. Fresh weight of C. falcata seedlings was significantly increased by the tripartite symbioses even in those with no shoot, thus providing further evidence for the mycoheterotrophic nature of this orchid. The achievement of seedling culture in tripartite symbioses would be valuable for conserving many forest orchids and for conducting experiments to understand their physiology and ecology.
著者
Nakabo Tetsuji Nakayama Kouji Muto Nozomu MIYAZAWA Masayuki
出版者
Springer Japan
雑誌
Ichthyological research (ISSN:13418998)
巻号頁・発行日
vol.58, no.2, pp.180-183, 2011-04-25
参考文献数
34
被引用文献数
6

田沢湖で絶滅した固有種クニマス(サケ科)の山梨県西湖での発見. 京都大学プレスリリース. 2011-02-22.Oncorhynchus kawamurae (Osteichthyes: Salmonidae) (common name "Kunimasu"), a species endemic to Lake Tazawa, Akita Prefecture, Japan, was believed to have been extinct since 1940. However, nine specimens were discovered in March and April 2010 in Lake Saiko, Yamanashi Prefecture, one of the lakes to which eyed eggs of the species were introduced in 1935. These were identified as O. kawamurae because of having 47–62 pyloric caeca, 37–43 gill-rakers, a black-colored body, and spawning at 30–40 m depth in early spring, which are unique characteristics within Oncorhynchus. Furthermore, the distinctiveness of Kunimasu from sympatric kokanee (O. nerka) was supported by microsatellite DNA data.
著者
Matsuura Hiroshi Yazaki Ikuko Okino Tatsufumi
出版者
Springer Japan
雑誌
Fisheries Science (ISSN:09199268)
巻号頁・発行日
vol.75, no.3, pp.777-783, 2009-05
被引用文献数
25

Larval metamorphosis inducers of the sea cucumber Apostichopus japonicus were screened from physiologically active compounds. Doliolaria larvae completed their metamorphosis to juveniles in 120 hours when treated with 5-10 μM of dopamine and L-DOPA, and 50 μM of L-adrenaline and L-noradrenaline. Doliolaria larvae had to be exposed to dopamine or L-DOPA for at least 24 h. D1-like dopamine receptor antagonists SKF87566 and LE300 (10 μM) inhibited metamorphosis by dopamine. However, the D2-like dopamine receptor antagonists sulpiride and nemonapride (10 μM) did not inhibit the effect of dopamine. The results suggest that D1-like dopamine receptors are involved in larval metamorphosis of the sea cucumber A. japonicus.
著者
Basri Rehana Yabe Ichiro Soma Hiroyuki Sasaki Hidenao
出版者
Springer Japan
雑誌
Journal of Human Genetics (ISSN:14345161)
巻号頁・発行日
vol.52, no.10, pp.848-855, 2007-10
被引用文献数
35

Autosomal dominant cerebellar ataxia (ADCA) is a genetically heterogeneous group of neurodegenerative disorders. To shed further light on the clinical and genetic spectrum of ADCA in Japan, we conducted a study to determine the frequency of a new variety of different subtypes of SCAs among ADCA patients. This current study was carried out from April 1999 to December 2006 on the basis of patients with symptoms and signs of ADCA disorders. PCR and/or direct sequencing were evaluated in a total of 113 families. Among them, 35 families were found to have the mutation associated with SCA6, 30 with SCA3, 11 with SCA1, five with SCA2, five with DRPLA, and one with SCA14. We also detected the heterozygous −16C → T single nucleotide substitution within the puratrophin-1 gene responsible for 16q22.1-linked ADCA in ten families. In this study, unusual varieties of SCA, including 27, 13, 5, 7, 8, 12, 17, and 16 were not found. Of the 113 patients, 14% had as yet unidentified ADCA mutations. The present study validates the prevalence of genetically distinct ADCA subtypes based on ethnic origin and geographical variation, and shows that 16q-linked ADCA has strong hereditary effects in patients with ADCAs in Japan.
著者
Oshiro Yukio Sasaki Ryoko Takeguchi Takaya Ibukuro Kenji Ohkohchi Nobuhiro
出版者
Springer Japan
雑誌
Journal of hepato-biliary-pancreatic sciences (ISSN:18686974)
巻号頁・発行日
vol.20, no.6, pp.639-646, 2013-08
被引用文献数
4

Background/purposeTo date there have been only a few radiological studies of the caudate artery. This study aimed to precisely analyze the caudate artery as well as the relationship between the caudate arteries, the arterial plexus at the hilar plate, and the hilar bile duct.MethodsReconstructed three-dimensional (3D) computed tomography images from 50 patients during hepatic arteriography were analyzed. The caudate arteries were classified as right branches (Irs) or left branches (Ils). The communicating artery (CA) was defined as the artery connecting the right, left, segmental, and common hepatic arteries.ResultsThe caudate artery was divided into 3 types: an independent branch (Type 1); the common tract formed by Ir and Il (Type 2); and an arterial branch from the CA (Type 3). The CA was recognized in 25 of 50 patients. There was a total of 65 arteries to the hilar bile duct observed in 40 patients, and 24 (37 %) of these 65 arteries to the hilar bile duct originated from the caudate artery or CA.ConclusionThe caudate artery plays an important role not only in connecting the blood supply of the right and left livers but in the blood supply to the hilar bile duct.
著者
Yamamoto Tsuyoshi Nakamura Atsuko Iwai Hiroaki Ishii Tadashi Ma Jian Feng Yokoyama Ryusuke Nishitani Kazuhiko Satoh Shinobu Furukawa Jun
出版者
Springer Japan
雑誌
Journal of plant research (ISSN:09189440)
巻号頁・発行日
vol.125, no.6, pp.771-779, 2012-11
被引用文献数
64 3

Rice (Oryza sativa L.) is a typical Si-accumulating plant and is able to accumulate Si up to >10 % of shoot dry weight. The cell wall has been reported to become thicker under Si-deficient condition. To clarify the relationship between Si accumulation and cell wall components, the physical properties of, and macromolecular components and Si content in, the pectic, hemicellulosic, and cellulosic fractions prepared from rice seedlings grown in hydroponics with or without 1.5 mM silicic acid were analyzed. In the absence of Si (the −Si condition), leaf blades drooped, but physical properties were enhanced. Sugar content in the cellulosic fraction and lignin content in the total cell wall increased under −Si condition. After histochemical staining, there was an increase in cellulose deposition in short cells and the cell layer just beneath the epidermis in the −Si condition, but no significant change in the pattern of lignin deposition. Expression of the genes involved in secondary cell wall synthesis, OsCesA4, OsCesA7, OsPAL, OsCCR1 and OsCAD6 was up-regulated under −Si condition, but expression of OsCesA1, involved in primary cell wall synthesis, did not increase. These results suggest that an increase in secondary cell wall components occurs in rice leaves to compensate for Si deficiency.
著者
Motojima Hideko Yamada Parida Irie Mitsuteru Ozaki Masuo Shigemori Hideyuki Isoda Hiroko
出版者
Springer Japan
雑誌
Journal of material cycles and waste management (ISSN:14384957)
巻号頁・発行日
vol.14, no.3, pp.169-180, 2012-09
被引用文献数
14

In this study, the main characteristics and soil amendment effects on the saline–alkali soil of humic acid extracted from solubilized excess sludge (SS-HA) were investigated. The excess sludge was solubilized prior to extraction to improve the humic acid recovery rate. The structural features of SS-HA were characterized by an elemental analysis, Fourier transform infrared spectroscopy, and 1H-nuclear magnetic resonance spectroscopy, and compared with those of HA extracted from non-solubilized excess sludge (ES-HA). The results showed that extraction efficiency of humic acid was enhanced by using solubilization, although structural properties of humic acid extracted from solubilized excess sludge were almost the same as those of ES-HA. To study a utilization method of SS-HA, the soil amendment effects on saline–alkali soil by mixture of SS-HA were investigated with a model soil-column experiment. SS-HA reduced the pH of the saline–alkali soil, and the effect was immediately observed or faster than the case in which only peat is added. Moreover, the cation exchangeable capacity of the saline–alkali soil was enhanced by addition of SS-HA.